Ethidium Homodimer-1 (EthD-1)
Ethidium Homodimer-1 (EthD-1)
Citations & References (225)
Invitrogen™

Ethidium Homodimer-1 (EthD-1)

The cell-impermeant viability indicator ethdium homodimer-1 (EthD-1) is a high-affinity nucleic acid stain that is weakly fluorescent until bound toRead more
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Catalog NumberQuantity
E11691 mg
Catalog number E1169
Price (HKD)
4,242.00
Each
Add to cart
Quantity:
1 mg
Price (HKD)
4,242.00
Each
Add to cart

The cell-impermeant viability indicator ethdium homodimer-1 (EthD-1) is a high-affinity nucleic acid stain that is weakly fluorescent until bound to DNA and emits red fluorescence (Excitation/emission maxima ∼528/617 nm).

We recommend using high-quality anhydrous DMSO for a stock concentration of 1 to 2 mM in DMSO/H2O 1:4 (v/v).



Visualize staining your cell without wasting your reagents, antibodies, or time with our new Stain-iT Cell Staining Simulator.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorOrange
DescriptionEthidium Homodimer-1 (EthD-1)
Detection MethodFluorescence
Dye TypeCell-Impermeant
Emission617 nm
Excitation Wavelength Range528 nm
For Use With (Application)Cell staining assays
For Use With (Equipment)Fluorescence Microscope
Quantity1 mg
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeViability Indicator
SubCellular LocalizationNucleic Acids, Nucleus
Unit SizeEach
Contents & Storage
Store in freezer (-5°C to -30°C) and protect from light.

Frequently asked questions (FAQs)

I am doing a Live/Dead assay using Calcein, AM, for live cells and ethidium homodimer-1 for dead cells. Can I fix the cells after labeling and retain the staining?

This is not recommended. Neither Calcein nor ethidium homodimer-1 bind to any cellular components upon fixation. There is no guarantee that the dyes will be retained upon fixation or any subsequent wash steps. We recommend scoring for live and dead cells as soon as possible after staining.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (225)

Citations & References
Abstract
Enzymatic isolation and characterization of single vascular smooth muscle cells from cremasteric arterioles.
Authors:Jackson WF,Huebner JM,Rusch NJ
Journal:Microcirculation (New York, N.Y. : 1994)
PubMed ID:9110282
OBJECTIVE: The goal of the present study was to develop a method to isolate viable arteriolar muscle cells from single cremasteric arterioles, which retain the contractile and electrophysiological phenotype of the donor microvessels. METHODS: Arterioles were hand-dissected from rat and hamster cremaster muscles and dissociated by incubation in papain and ... More
Enzymatic isolation and characterization of single vascular smooth muscle cells from cremasteric arterioles.
Authors:Jackson WF,Huebner JM,Rusch NJ
Journal:Microcirculation (New York, N.Y. : 1994)
PubMed ID:8930888
OBJECTIVE: The goal of the present study was to develop a method to isolate enzymatically viable arteriolar muscle cells from single cremasteric arterioles, which retain the contractile and electrophysiological phenotype of the donor microvessels. METHODS: Arterioles were hand-dissected from rat and hamster cremaster muscles and dissociated by incubation in papain ... More
A stable double-stranded DNA-ethidium homodimer complex: application to picogram fluorescence detection of DNA in agarose gels.
Authors:Glazer AN, Peck K, Mathies RA
Journal:Proc Natl Acad Sci U S A
PubMed ID:2339125
The complex between double-stranded DNA and ethidium homodimer (5,5'-diazadecamethylene)bis(3,8-diamino-6-phenylphenanthridini um) cation, formed at a ratio of 1 homodimer per 4 or 5 base pairs, is stable in agarose gels under the usual conditions for electrophoresis. This unusual stability allows formation of the complex before electrophoresis and then separation and detection ... More
Successful storage of peripheral nerve before transplantation using green tea polyphenol: an experimental study in rats.
Authors:Ikeguchi R, Kakinoki R, Okamoto T, Matsumoto T, Hyon SH, Nakamura T
Journal:Exp Neurol
PubMed ID:14769360
Green tea polyphenol is known to act as a buffer, reducing biological responses to oxidative stress. Several effects of polyphenol have been reported, such as protection of tissue from ischemia, antineoplasmic and anti-inflammatory effects, and suppression of arteriosclerosis. In this study, we investigated whether peripheral nerve segments could be kept ... More
Indirubin enhances tumor necrosis factor-induced apoptosis through modulation of nuclear factor-kappa B signaling pathway.
Authors:Sethi G, Ahn KS, Sandur SK, Lin X, Chaturvedi MM, Aggarwal BB
Journal:J Biol Chem
PubMed ID:16785236
'Although indirubin is known to exhibit anti-cancer and anti-inflammatory activities, very little is known about its mechanism of action. In this study, we investigated whether indirubin mediates its effects through interference with the NF-kappaB pathway. As examined by the DNA binding of NF-kappaB, we found that indirubin suppressed tumor necrosis ... More
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