Invitrogen™

      SYTOX™ AADvanced™ Dead Cell Stain Kit

      SYTOX™ AADvanced™ Dead Cell Stain Kit

      Catalog number:  S10349

       Related applications: Flow Cytometry

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      Description

      SYTOX™ AADvanced™ Dead Cell Stain (S10274, S10349) is a new high-affinity nucleic acid stain for the detection of dead cells and analysis of cell cycle using the common 488 nm blue laser in flow cytometry. The dye is spectrally similar to 7-AAD but with rapid kinetics of uptake and relatively low CVs. SYTOX™ AADvanced™ Dead Cell Stain penetrates cells more efficiently than 7-AAD providing better separation of live and dead cells. SYTOX™ AADvanced™ Dead Cell Stain can also be used with fixed cells for DNA content analysis when paired with RNAse treatment. SYTOX™ AADvanced™ Dead Cell Stain Kits contain a vial(s) of dried dye and anhydrous DMSO providing stable shelf life.

      View a selection guide for all nonfixable viability dyes for flow cytometry.
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      For Use With (Equipment): Flow Cytometer
      Cell Permeability: Cell-Impermeant
      Sub-Cellular Localization: Nucleic Acids, Nucleus
      Detection Method: Fluorescent
      Excitation⁄Emission (nm): 546⁄647
      Flow Cytometer Laser Lines: 488, 532, 561
      Form: Solid
      Format: Tube(s)
      Label or Dye: SYTOX® AADvanced™
      Number of Reactions: 100
      Product Line: Molecular Probes®, SYTOX®
      Product Size: 1 kit
      Solubility: DMSO (Dimethylsulfoxide)
      Shipping Condition: Room Temperature

      Contents & storage

      Contains 1 vial of SYTOX® AADvanced™ dead cell stain and 200 µL dimethylsulfoxide (DMSO).

      Store at ≤-20°C and protected from light.

      Documents

      Manuals & protocols