Invitrogen™
SYTOX™ AADvanced™ Dead Cell Stain Kit
Description
SYTOX™ AADvanced™ Dead Cell Stain (S10274, S10349) is a new high-affinity nucleic acid stain for the detection of dead cells and analysis of cell cycle using the common 488 nm blue laser in flow cytometry. The dye is spectrally similar to 7-AAD but with rapid kinetics of uptake and relatively low CVs. SYTOX™ AADvanced™ Dead Cell Stain penetrates cells more efficiently than 7-AAD providing better separation of live and dead cells. SYTOX™ AADvanced™ Dead Cell Stain can also be used with fixed cells for DNA content analysis when paired with RNAse treatment. SYTOX™ AADvanced™ Dead Cell Stain Kits contain a vial(s) of dried dye and anhydrous DMSO providing stable shelf life.View a selection guide for all nonfixable viability dyes for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.
Figures
A mixed population of Jurkat cells were stained with SYTOX® AADvanced™ Dead Cell Stain Kit (S10349) and analyzed by flow cytometry
Caspase activity detection in Jurkat cells using the CellEvent® Caspase-3/7 Green Flow Cytometry Assay Kit on the Attune® Flow Cytometer.
Multiparameter apoptosis assay.
Specifications
| Cell Permeability: | Cell-Impermeant |
|---|---|
| Detection Method: | Fluorescent |
| Excitation/Emission: | 546⁄647 |
| Flow Cytometer Laser Lines: | 488, 561, 532 |
| For Use With (Equipment): | Flow Cytometer |
| Form: | Solid |
| Label or Dye: | SYTOX™ AADvanced™ |
| No. of Reactions: | 100 |
| Product Line: | Molecular Probes™, SYTOX™ |
| Shipping Condition: | Room Temperature |
| Solubility: | DMSO (Dimethylsulfoxide) |
| Sub Cellular Localization: | Nucleus, Nucleic Acids |
| Format: | Tube(s) |
| No. of Tests: | 100 rxn(s) |
Contents & storage
Contains 1 vial of SYTOX™ AADvanced™ dead cell stain and 200 µL dimethylsulfoxide (DMSO).
Store at ≤-20°C and protected from light.
Store at ≤-20°C and protected from light.
