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CellTracker™ Orange CMTMR Dye

Catalog number: C2927

Molecular Probes™  Related applications: Cell Tracing & Tracking | Cell Viability, Proliferation & Function | Cellular Imaging | Target-Based ADME⁄Tox Assays

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Description

CellTracker™ Orange CMTMR (5-(and-6)-(((4-chloromethyl)benzoyl)amino)tetramethylrhodamine) (mixed isomers) is a fluorescent dye well suited for monitoring cell movement or location. This dye is well retained, allowing for multigenerational tracking of cellular movements. And the orange excitation/emission spectra are ideal for multiplexing with green fluorescent dyes and proteins.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

• Easy to use—remove medium, add dye, incubate 30 minutes, and image cells
• Fluorescent signal retention of >72 hours (typically three to six generations)
• Orange excitation/emission spectra (541/565 nm maxima) ideal for multiplexing
• Low cytotoxicity—does not affect viability or proliferation

CellTracker™ Orange CMTMR fluorescent dye has been designed to freely pass through cell membranes into cells, where it is transformed into cell membrane-impermeant reaction products. CellTracker™ Orange CMTMR dye is retained in living cells through several generations. The dye is transferred to daughter cells, but not adjacent cells in a population. CellTracker™ Orange CMTMR dye is designed to display fluorescence for at least 72 hours, and the dye exhibits ideal tracking properties: it is stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. Additionally, the excitation and emission spectra of CellTracker™ Orange CMTMR dye are well separated from GFP (green fluorescent protein) and spectra allowing for multiplexing.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Reagent Type: Cell Labeling Reagents, Cell Tracker Compounds
Product Size: 1 mg
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.

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Manuals & protocols

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