Evaluation of a flow cytometric fluorescence quenching assay of phagocytosis of sensitized sheep erythrocytes by polymorphonuclear leukocytes.
AuthorsVan Amersfoort ES, Van Strijp JA
JournalCytometry
PubMed ID7875036
A number of reports have been published describing phagocytosis assays for flow cytometric analysis. In some of these, the fluorescence quenching technique has been used to discriminate between adherent and ingested particles. In this report, we have evaluated the efficacy of a quantitative fluorescence quenching technique with crystal violet and ... More
Single-molecule analysis of cadherin-mediated cell-cell adhesion.
AuthorsPanorchan P, Thompson MS, Davis KJ, Tseng Y, Konstantopoulos K, Wirtz D
JournalJ Cell Sci
PubMed ID16371651
'Cadherins are ubiquitous cell surface molecules that are expressed in virtually all solid tissues and localize at sites of cell-cell contact. Cadherins form a large and diverse family of adhesion molecules, which play a crucial role in a multitude of cellular processes, including cell-cell adhesion, motility, and cell sorting in ... More
Inhibition of angiogenesis and metastases of the Lewis-lung cell carcinoma by the quinoline-3-carboxamide, Linomide.
AuthorsBorgström P, Torres Filho IP, Hartley-Asp B
JournalAnticancer Res
PubMed ID7544090
'Linomide has antitumor effects when administered in vivo but not in vitro. Recent data indicate that at least part of this effect can be attributed to anti-angiogenic properties. The aim of the present investigation was to study the anti-angiogenic effects of Linomide on early tumor-induced angiogenesis in vivo, using a ... More
Stochastic regulation of cell migration from the efferent lymph to oxazolone-stimulated skin.
AuthorsWest CA, He C, Su M, Rawn J, Swanson S, Hay JB, Mentzer SJ
JournalJ Immunol
PubMed ID11160191
'The systemic immune response is a dynamic process involving the trafficking of lymphocytes from the Ag-stimulated lymph node to the peripheral tissue. Studies in sheep have demonstrated several phases of cell output in the efferent lymph after Ag stimulation. When skin contact sensitizers are used as Ag, the efferent lymph ... More
T-cell priming by dendritic cells in lymph nodes occurs in three distinct phases.
AuthorsMempel TR, Henrickson SE, Von Andrian UH
JournalNature
PubMed ID14712275
'Primary T-cell responses in lymph nodes (LNs) require contact-dependent information exchange between T cells and dendritic cells (DCs). Because lymphocytes continually enter and leave normal LNs, the resident lymphocyte pool is composed of non-synchronized cells with different dwell times that display heterogeneous behaviour in mouse LNs in vitro. Here we ... More
Endothelial selectins and alpha4 integrins regulate independent pathways of T lymphocyte recruitment in the pulmonary immune response.
AuthorsWolber FM, Curtis JL, Mály P, Kelly RJ, Smith P, Yednock TA, Lowe JB, Stoolman LM
JournalJ Immunol
PubMed ID9780218
'The cell adhesion molecules (CAMs) required for T lymphocyte recruitment during pulmonary immune responses have not been defined. Our laboratories recently reported that intratracheal (IT) challenge of sensitized mice with SRBC induced prolonged expression of vascular P-selectin, E-selectin, and VCAM-1, particularly in areas of mononuclear leukocyte infiltration. A surge in ... More
Covalent surface chemistry gradients for presenting bioactive peptides.
AuthorsKipper MJ, Kleinman HK, Wang FW
JournalAnal Biochem
PubMed ID17339030
'The activation of surfaces by covalent attachment of bioactive moieties is an important strategy for improving the performance of biomedical materials. Such techniques have also been used as tools to study cellular responses to particular chemistries of interest. The creation of gradients of covalently bound chemistries is a logical extension ... More
Interaction of the selectin ligand PSGL-1 with chemokines CCL21 and CCL19 facilitates efficient homing of T cells to secondary lymphoid organs.
AuthorsVeerman KM, Williams MJ, Uchimura K, Singer MS, Merzaban JS, Naus S, Carlow DA, Owen P, Rivera-Nieves J, Rosen SD, Ziltener HJ
JournalNat Immunol
PubMed ID17401367
'P-selectin glycoprotein ligand 1 (PSGL-1) is central to the trafficking of immune effector cells to areas of inflammation through direct interactions with P-selectin, E-selectin and L-selectin. Here we show that PSGL-1 was also required for efficient homing of resting T cells to secondary lymphoid organs but functioned independently of selectin ... More
Receptor tyrosine kinase ErbB4 modulates neuroblast migration and placement in the adult forebrain.
AuthorsAnton ES, Ghashghaei HT, Weber JL, McCann C, Fischer TM, Cheung ID, Gassmann M, Messing A, Klein R, Schwab MH, Lloyd KC, Lai C
JournalNat Neurosci
PubMed ID15543145
'Neural progenitor proliferation, differentiation and migration are continually active in the rostral migratory stream of the adult brain. Here, we show that the receptor tyrosine kinase ErbB4 is expressed prominently by the neuroblasts present in the subventricular zone and the rostral migratory stream. The neuregulins (NRG1-NRG3), which have been identified ... More
Cell tracing dyes significantly change single cell mechanics.
AuthorsLulevich V, Shih YP, Lo SH, Liu GY,
JournalJ Phys Chem B
PubMed ID19366241
'Cell tracing dyes are very frequently utilized in cellular biology research because they provide highly sensitive fluorescent tags that do not compromise cellular functions such as growth and proliferation. In many investigations concerning cellular adhesion and mechanics, fluorescent dyes have been employed with the assumption of little impact on the ... More
The power of single and multibeam two-photon microscopy for high-resolution and high-speed deep tissue and intravital imaging.
AuthorsNiesner R, Andresen V, Neumann J, Spiecker H, Gunzer M,
JournalBiophys J
PubMed ID17557785
'Two-photon microscopy is indispensable for deep tissue and intravital imaging. However, current technology based on single-beam point scanning has reached sensitivity and speed limits because higher performance requires higher laser power leading to sample degradation. We utilize a multifocal scanhead splitting a laser beam into a line of 64 foci, ... More
Use of CMFDA and CMTMR fluorescent dyes in FACS-based antibody screening.
AuthorsYang XP, Gallo M, Ngan I, Nocerini M, Chen MM
JournalBiotechniques
PubMed ID11911670
'Cell-based immunizations are often used when membrane antigens are difficult to purify. To confirm that an antibody binding to the surface of a cell line is, in fact, binding to the desired antigen, FACS can be performed independently on two cell lines, a transfected cell line expressing the antigen of ... More
Molecular dynamics in living cells observed by fluorescence correlation spectroscopy with one- and two-photon excitation.
AuthorsSchwille P, Haupts U, Maiti S, Webb WW
JournalBiophys J
PubMed ID10512844
'Multiphoton excitation (MPE) of fluorescent probes has become an attractive alternative in biological applications of laser scanning microscopy because many problems encountered in spectroscopic measurements of living tissue such as light scattering, autofluorescence, and photodamage can be reduced. The present study investigates the characteristics of two-photon excitation (2PE) in comparison ... More
Optimal inhibition of X4 HIV isolates by the CXC chemokine stromal cell-derived factor 1 alpha requires interaction with cell surface heparan sulfate proteoglycans.
AuthorsValenzuela-Fernández A, Palanche T, Amara A, Magerus A, Altmeyer R, Delaunay T, Virelizier JL, Baleux F, Galzi JL, Arenzana-Seisdedos F
JournalJ Biol Chem
PubMed ID11352904
'The chemokine stromal cell-derived factor 1 (SDF-1) is the natural ligand for CXC chemokine receptor 4 (CXCR4). SDF-1 inhibits infection of CD4+ cells by X4 (CXCR4-dependent) human immunodeficiency virus (HIV) strains. We previously showed that SDF-1 alpha interacts specifically with heparin or heparan sulfates (HSs). Herein, we delimited the boundaries ... More
Comparison of the A2 gene locus in Leishmania donovani and Leishmania major and its control over cutaneous infection.
AuthorsZhang WW, Mendez S, Ghosh A, Myler P, Ivens A, Clos J, Sacks DL, Matlashewski G
JournalJ Biol Chem
PubMed ID12829719
'In Old World Leishmania infections, Leishmania donovani is responsible for fatal visceral leishmaniasis, and L. major is responsible for non-fatal cutaneous leishmaniasis in humans. The genetic differences between these species which govern the pathology or site of infection are not known. We have therefore carried out detailed analysis of the ... More
Perforin-dependent elimination of dendritic cells regulates the expansion of antigen-specific CD8+ T cells in vivo.
AuthorsYang J, Huck SP, McHugh RS, Hermans IF, Ronchese F
JournalProc Natl Acad Sci U S A
PubMed ID16373503
'The lifespan and survival of dendritic cells (DC) in vivo are potentially critical to the expansion of T cell immune responses. We have previously reported that DC loaded with specific antigen are rapidly eliminated by cytotoxic T lymphocytes (CTL) in vivo, but the site, mechanism, and consequences of DC elimination ... More
Spontaneous regression of advanced cancer: identification of a unique genetically determined, age-dependent trait in mice.
AuthorsCui Z, Willingham MC, Hicks AM, Alexander-Miller MA, Howard TD, Hawkins GA, Miller MS, Weir HM, Du W, DeLong CJ
JournalProc Natl Acad Sci U S A
PubMed ID12724523
'We have established and studied a colony of mice with a unique trait of host resistance to both ascites and solid cancers induced by transplantable cells. One dramatic manifestation of this trait is age-dependent spontaneous regression of advanced cancers. This powerful resistance segregates as a single-locus dominant trait, is independent ... More
Evidence for a conserved role for CRKII and Rac in engulfment of apoptotic cells.
AuthorsTosello-Trampont AC, Brugnera E, Ravichandran KS
JournalJ Biol Chem
PubMed ID11297528
'Apoptosis or programmed cell death occurs in multicellular organisms throughout life. The removal of apoptotic cells by phagocytes prevents secondary necrosis and inflammation and also plays a key role in tissue remodeling and regulating immune responses. The molecular mechanisms that regulate the engulfment of apoptotic cells are just beginning to ... More
Contribution of dermal macrophage trafficking in the sensitization phase of contact hypersensitivity.
AuthorsSato K, Imai Y, Irimura T
JournalJ Immunol
PubMed ID9862715
'We investigated cellular trafficking of dermal macrophages that express a macrophage calcium-type lectin (MMGL) during the sensitization of delayed-type hypersensitivity. In skin, dermal macrophages, but not epidermal Langerhans cells, have been shown to express MMGL. Epicutaneous sensitization by FITC produced a transient increase in MMGL-positive cells in regional lymph nodes. ... More
Vascular lipid accumulation, lipoprotein oxidation, and macrophage lipid uptake in hypercholesterolemic zebrafish.
AuthorsStoletov K, Fang L, Choi SH, Hartvigsen K, Hansen LF, Hall C, Pattison J, Juliano J, Miller ER, Almazan F, Crosier P, Witztum JL, Klemke RL, Miller YI,
JournalCirc Res
PubMed ID19265037
'Lipid accumulation in arteries induces vascular inflammation and atherosclerosis, the major cause of heart attack and stroke in humans. Extreme hyperlipidemia induced in mice and rabbits enables modeling many aspects of human atherosclerosis, but microscopic examination of plaques is possible only postmortem. Here we report that feeding adult zebrafish (Danio ... More
Cytotoxic T lymphocytes kill multiple targets simultaneously via spatiotemporal uncoupling of lytic and stimulatory synapses.
AuthorsWiedemann A, Depoil D, Faroudi M, Valitutti S
JournalProc Natl Acad Sci U S A
PubMed ID16832064
'A longstanding paradox in the activation of cytotoxic T lymphocytes (CTL) arises from the observation that CTL recognize and rapidly destroy target cells with exquisite sensitivity despite the fact that cytokine production requires sustained signaling at the immunological synapse. Here we solve this paradox by showing that CTL establish sustained ... More
Distinct T cell dynamics in lymph nodes during the induction of tolerance and immunity.
'Induction of immunity and peripheral tolerance requires contacts between antigen-bearing dendritic cells (DCs) and cognate T cells. Using real-time two-photon microscopy, we have analyzed the dynamics of CD8(+) T cells in lymph nodes during the induction of antigen-specific immunity or tolerance. At 15-20 h after the induction of immunity, T ... More
CX3CR1-fractalkine expression regulates cellular mechanisms involved in adhesion, migration, and survival of human prostate cancer cells.
AuthorsShulby SA, Dolloff NG, Stearns ME, Meucci O, Fatatis A
JournalCancer Res
PubMed ID15256432
'Chemokines and their receptors might be involved in the selection of specific organs by metastatic cancer cells. For instance, the CXCR4-SDF-1alpha pair regulates adhesion and migration of breast as well as prostate cancer cells to metastatic sites. In this study, we present the first evidence for the expression of CX3CR1--the ... More
Targeting microspheres and cells to polyethylene glycol-modified biological surfaces.
AuthorsDeglau TE, Johnson JD, Villanueva FS, Wagner WR
JournalJ Biomed Mater Res A
PubMed ID17177289
'It has previously been demonstrated that damaged arterial tissue can be acutely modified with protein-reactive polyethylene glycol (PEG) to block undesirable platelet deposition. This concept might be expanded by employing PEG-biotin and its strong interaction with avidin for site-specific targeted delivery. Toward this end, cultured endothelial cells (ECs) were surface ... More
Burkholderia pseudomallei induces cell fusion and actin-associated membrane protrusion: a possible mechanism for cell-to-cell spreading.
AuthorsKespichayawattana W, Rattanachetkul S, Wanun T, Utaisincharoen P, Sirisinha S
JournalInfect Immun
PubMed ID10948167
'Burkholderia pseudomallei, a facultative intracellular bacterium, is the causative agent of a broad spectrum of diseases collectively known as melioidosis. Its ability to survive inside phagocytic and nonphagocytic cells and to induce multinucleated giant cell (MNGC) formation has been demonstrated. This study was designed to assess a possible mechanism(s) leading ... More
Phagocytosis of necrotic cells by macrophages is phosphatidylserine dependent and does not induce inflammatory cytokine production.
AuthorsBrouckaert G, Kalai M, Krysko DV, Saelens X, Vercammen D, Ndlovu M, Haegeman G, D'Herde K, Vandenabeele P
JournalMol Biol Cell
PubMed ID14668480
'Apoptotic cells are cleared by phagocytosis during development, homeostasis, and pathology. However, it is still unclear how necrotic cells are removed. We compared the phagocytic uptake by macrophages of variants of L929sA murine fibrosarcoma cells induced to die by tumor necrosis factor-induced necrosis or by Fas-mediated apoptosis. We show that ... More
Neutralizing anti-vascular endothelial growth factor antibody completely inhibits angiogenesis and growth of human prostate carcinoma micro tumors in vivo.
AuthorsBorgström P, Bourdon MA, Hillan KJ, Sriramarao P, Ferrara N
JournalProstate
PubMed ID9537593
'BACKGROUND: Neovascularization mediated by growth factors produced by tumors is critical for the growth of tumors. Vascular endothelial growth factor (VEGF) is one such growth factor. A neutralizing anti-VEGF antibody (A4.6.1) was recently shown in vivo to inhibit tumor angiogenesis and growth of the human rhabdomyosarcoma cell line A673. The ... More
Specific recognition of apoptotic cells reveals a ubiquitous and unconventional innate immunity.
AuthorsCvetanovic M, Mitchell JE, Patel V, Avner BS, Su Y, van der Saag PT, Witte PL, Fiore S, Levine JS, Ucker DS
JournalJ Biol Chem
PubMed ID16707494
'The purpose of physiological cell death is the noninflammatory clearance of cells that have become inappropriate or nonfunctional. Consistent with this function, the recognition of apoptotic cells by professional phagocytes, including macrophages and dendritic cells, triggers a set of potent anti-inflammatory responses manifest on multiple levels. The immediate-early inhibition of ... More
CD69 acts downstream of interferon-alpha/beta to inhibit S1P1 and lymphocyte egress from lymphoid organs.
AuthorsShiow LR, Rosen DB, Brdicková N, Xu Y, An J, Lanier LL, Cyster JG, Matloubian M
JournalNature
PubMed ID16525420
'Naive lymphocytes continually enter and exit lymphoid organs in a recirculation process that is essential for immune surveillance. During immune responses, the egress process can be shut down transiently. When this occurs locally it increases lymphocyte numbers in the responding lymphoid organ; when it occurs systemically it can lead to ... More
Flow cytometric evaluation of a model for phagocytosis of cells undergoing apoptosis.
AuthorsSchrijvers DM, Martinet W, De Meyer GR, Andries L, Herman AG, Kockx MM
JournalJ Immunol Methods
PubMed ID15099759
'Phagocyte recognition of cells undergoing apoptosis is a rapid, efficient way of removing unwanted cells from tissue. The uptake of apoptotic cells prevents the release of potentially toxic cell contents that might otherwise damage neighbouring cells and elicit an inflammatory response. The aim of this work was to evaluate a ... More
Cholinergic expression by a neural stem cell line grafted to the adult medial septum/diagonal band complex.
AuthorsDoering LC, Snyder EY
JournalJ Neurosci Res
PubMed ID10972956
'The potential of a neural stem cell line to acquire cholinergic characteristics was studied in transplants injected into the septum/diagonal band nuclei of young adult rats and mice. The stem cells integrated within the nuclei and survived for up to 9 months. Three methods were used to identify the grafted ... More
Trogocytosis-based generation of suppressive NK cells.
'Trogocytosis is a fast uptake of membranes and associated molecules from one cell by another. Trogocytosis between natural killer (NK) cells and tumors is already described, but the functional relevance of NK-tumor targets material exchange is unclear. We investigated whether the immunosuppressive molecule HLA-G that is commonly expressed by tumors ... More
Ventricle-directed migration in the developing cerebral cortex.
AuthorsNadarajah B, Alifragis P, Wong RO, Parnavelas JG
JournalNat Neurosci
PubMed ID11850632
'It is believed that postmitotic neurons migrate away from their sites of origin in the germinal zones to populate distant targets. Contrary to this notion, we found, using time-lapse imaging of brain slices, populations of neurons positioned at various levels of the developing neocortex that migrate towards the cortical ventricular ... More
Mannose receptor regulates myoblast motility and muscle growth.
AuthorsJansen KM, Pavlath GK
JournalJ Cell Biol
PubMed ID16864654
'Myoblast fusion is critical for the formation, growth, and maintenance of skeletal muscle. The initial formation of nascent myotubes requires myoblast-myoblast fusion, but further growth involves myoblast-myotube fusion. We demonstrate that the mannose receptor (MR), a type I transmembrane protein, is required for myoblast-myotube fusion. Mannose receptor (MR)-null myotubes were ... More
Regulation of dendritic cell migration to the draining lymph node: impact on T lymphocyte traffic and priming.
AuthorsMartIn-Fontecha A, Sebastiani S, Höpken UE, Uguccioni M, Lipp M, Lanzavecchia A, Sallusto F
JournalJ Exp Med
PubMed ID12925677
'Antigen-pulsed dendritic cells (DCs) are used as natural adjuvants for vaccination, but the factors that influence the efficacy of this treatment are poorly understood. We investigated the parameters that affect the migration of subcutaneously injected mouse-mature DCs to the draining lymph node. We found that the efficiency of DC migration ... More
The presumptive phosphatidylserine receptor is dispensable for innate anti-inflammatory recognition and clearance of apoptotic cells.
AuthorsMitchell JE, Cvetanovic M, Tibrewal N, Patel V, Colamonici OR, Li MO, Flavell RA, Levine JS, Birge RB, Ucker DS
JournalJ Biol Chem
PubMed ID16317002
'The role of the presumptive phosphatidylserine receptor (PSR) in the recognition and engulfment of apoptotic cells, and the antiinflammatory response they exert, has been of great interest. Genetic deficiency of PSR in the mouse is lethal perinatally, and results to date have been ambiguous with regard to the phagocytic and ... More
Quantitative 3D video microscopy of HIV transfer across T cell virological synapses.
AuthorsHübner W, McNerney GP, Chen P, Dale BM, Gordon RE, Chuang FY, Li XD, Asmuth DM, Huser T, Chen BK,
JournalScience
PubMed ID19325119
The spread of HIV between immune cells is greatly enhanced by cell-cell adhesions called virological synapses, although the underlying mechanisms have been unclear. With use of an infectious, fluorescent clone of HIV, we tracked the movement of Gag in live CD4 T cells and captured the direct translocation of HIV ... More
Viral protein U counteracts a human host cell restriction that inhibits HIV-1 particle production.
AuthorsVarthakavi V, Smith RM, Bour SP, Strebel K, Spearman P
JournalProc Natl Acad Sci U S A
PubMed ID14657387
Human cells resist viral infections by a variety of mechanisms. Viruses must overcome host cell restrictions to successfully reproduce their genetic material. Here, we identify a host restriction to viral replication that acts at the stage of particle assembly. Viral protein U (Vpu) is an HIV-1 accessory protein that enhances ... More
Two-photon tissue imaging: seeing the immune system in a fresh light.
AuthorsCahalan MD, Parker I, Wei SH, Miller MJ
JournalNat Rev Immunol
PubMed ID12415310
Many lymphocyte functions, such as antigen recognition, take place deep in densely populated lymphoid organs. Because direct in vivo observation was not possible, the dynamics of immune-cell interactions have been inferred or extrapolated from in vitro studies. Two-photon fluorescence excitation uses extremely brief (<1 picosecond) and intense pulses of light ... More
Two-photon imaging of lymphocyte motility and antigen response in intact lymph node.
AuthorsMiller MJ, Wei SH, Parker I, Cahalan MD
JournalScience
PubMed ID12016203
Lymphocyte motility is vital for trafficking within lymphoid organs and for initiating contact with antigen-presenting cells. Visualization of these processes has previously been limited to in vitro systems. We describe the use of two-photon laser microscopy to image the dynamic behavior of individual living lymphocytes deep within intact lymph nodes. ... More
Ninjurin, a novel adhesion molecule, is induced by nerve injury and promotes axonal growth.
AuthorsAraki T, Milbrandt J
JournalNeuron
PubMed ID8780658
Peripheral nerve injury results in axonal degeneration and in phenotypic changes of the surrounding Schwann cells, whose presence is critical for nerve regeneration. Using differential screening strategies, we identified a novel protein, termed ninjurin (for nerve injury-induced protein), that is up-regulated after axotomy in neurons and in Schwann cells surrounding ... More
Mixed infection of human U-937 cells by two different species of Leishmania.
AuthorsAbdullah SM, Flath B, Presber W
JournalAm J Trop Med Hyg
PubMed ID9715931
Mixed infections by different Leishmania species could explain differences in the clinical course of these infections. Moreover, mixed infections of the same macrophage could be the basis for parasite recombination. We stained three strains of Leishmania (L. mexicana amazonensis, L. donovani DD8, and L. infantum D2, respectively) with different fluorescent ... More
Fluorescent labelling of intracellular bacteria in living host cells.
AuthorsBoleti H, Ojcius DM, Dautry-Varsat A
JournalJ Microbiol Methods
PubMed ID10802143
The fluorescent reagent, CellTracker, labels metabolically-active cells and was used here to label Chlamydia in vivo during their exponential phase of growth in infected cells. HeLa cells infected with C. psittaci were labelled with the CellTracker reagents between 15 and 48 h post-infection. The fluorescent label accumulated in the host-cell ... More
Visualizing priming of virus-specific CD8+ T cells by infected dendritic cells in vivo.
The rational design of vaccines that elicit CD8+ T cell responses requires knowledge of the identity of the antigen-presenting cell (APC), the location and time of presentation and the nature of the antigen presented by the APC. Here we address these questions for an antigen encoded by a recombinant vaccinia ... More
ATP-mediated glia signaling.
AuthorsCotrina ML, Lin JH, López-García JC, Naus CC, Nedergaard M
JournalJ Neurosci
PubMed ID10751435
Glia calcium signaling has recently been identified as a potent modulator of synaptic transmission. We show here that the spatial expansion of calcium waves is mediated by ATP and subsequent activation of purinergic receptors. Ectopic expression of gap junction proteins, connexins (Cxs), leads to an increase in both ATP release ... More
Alpha4-integrin-VCAM-1 binding mediates G protein-independent capture of encephalitogenic T cell blasts to CNS white matter microvessels.
AuthorsVajkoczy P, Laschinger M, Engelhardt B
JournalJ Clin Invest
PubMed ID11518729
Direct in vivo evidence is still lacking for alpha4-integrin-mediated T cell interaction with VCAM-1 on blood-brain barrier-endothelium in experimental autoimmune encephalomyelitis (EAE). To investigate a possible alpha4-integrin-mediated interaction of encephalitogenic T cell blasts with VCAM-1 on the blood-brain barrier white matter endothelium in vivo, we have developed a novel spinal ... More
Dendritic cells express tight junction proteins and penetrate gut epithelial monolayers to sample bacteria.
AuthorsRescigno M, Urbano M, Valzasina B, Francolini M, Rotta G, Bonasio R, Granucci F, Kraehenbuhl JP, Ricciardi-Castagnoli P
JournalNat Immunol
PubMed ID11276208
Penetration of the gut mucosa by pathogens expressing invasion genes is believed to occur mainly through specialized epithelial cells, called M cells, that are located in Peyer's patches. However, Salmonella typhimurium that are deficient in invasion genes encoded by Salmonella pathogenicity island 1 (SPI1) are still able to reach the ... More
Investigating the mechanism of acoustically activated uptake of drugs from Pluronic micelles.
AuthorsHusseini GA, Runyan CM, Pitt WG
JournalBMC Cancer
PubMed ID12204099
BACKGROUND: This paper examines the mechanism of ultrasonic enhanced drug delivery from Pluronic micelles. In previous publications by our group, fluorescently labeled Pluronic was shown to penetrate HL-60 cells with and without the action of ultrasound, while drug uptake was increased with the application of ultrasound. METHODS: In this study, ... More
Transcription factor KLF2 regulates the migration of naive T cells by restricting chemokine receptor expression patterns.
AuthorsSebzda E, Zou Z, Lee JS, Wang T, Kahn ML,
JournalNat Immunol
PubMed ID18246069
The migration patterns of naive and activated T cells are associated with the expression of distinct sets of chemokine receptors, but the molecular basis for this regulation is unknown. Here we identify Krupple-like factor 2 (KLF2) as a key transcriptional factor needed to prevent naive T cells from expressing inflammatory ... More
Visualizing regulatory T cell control of autoimmune responses in nonobese diabetic mice.
AuthorsTang Q, Adams JY, Tooley AJ, Bi M, Fife BT, Serra P, Santamaria P, Locksley RM, Krummel MF, Bluestone JA,
JournalNat Immunol
PubMed ID16311599
The in vivo mechanism of regulatory T cell (T(reg) cell) function in controlling autoimmunity remains controversial. Here we have used two-photon laser-scanning microscopy to analyze lymph node priming of diabetogenic T cells and to delineate the mechanisms of T(reg) cell control of autoimmunity in vivo. Islet antigen-specific CD4(+)CD25(-) T helper ... More
Dscam and Sidekick proteins direct lamina-specific synaptic connections in vertebrate retina.
AuthorsYamagata M, Sanes JR,
JournalNature
PubMed ID18216854
Synaptic circuits in the retina transform visual input gathered by photoreceptors into messages that retinal ganglion cells (RGCs) send to the brain. Processes of retinal interneurons (amacrine and bipolar cells) form synapses on dendrites of RGCs in the inner plexiform layer (IPL). The IPL is divided into at least 10 ... More
A protease-dependent mechanism for initiating T-dependent B cell responses to large particulate antigens.
AuthorsCatron DM, Pape KA, Fife BT, van Rooijen N, Jenkins MK,
JournalJ Immunol
PubMed ID20208013
Ab production is critical for antimicrobial immunity, and the initial step in this process is the binding of Ag to the BCR. It has been shown that small soluble proteins can directly access the lymph node follicles to reach naive B cells, but virus particles must be translocated into follicles ... More
Microglia cells protect neurons by direct engulfment of invading neutrophil granulocytes: a new mechanism of CNS immune privilege.
AuthorsNeumann J, Sauerzweig S, Rönicke R, Gunzer F, Dinkel K, Ullrich O, Gunzer M, Reymann KG,
JournalJ Neurosci
PubMed ID18524901
Microglial cells maintain the immunological integrity of the healthy brain and can exert protection from traumatic injury. During ischemic tissue damage such as stroke, peripheral immune cells acutely infiltrate the brain and may exacerbate neurodegeneration. Whether and how microglia can protect from this insult is unknown. Polymorphonuclear neutrophils (PMNs) are ... More
Microfluidic control of cell pairing and fusion.
AuthorsSkelley AM, Kirak O, Suh H, Jaenisch R, Voldman J,
JournalNat Methods
PubMed ID19122668
Cell fusion has been used for many different purposes, including generation of hybridomas and reprogramming of somatic cells. The fusion step is the key event in initiation of these procedures. Standard fusion techniques, however, provide poor and random cell contact, leading to low yields. We present here a microfluidic device ... More
TLR ligand-induced podosome disassembly in dendritic cells is ADAM17 dependent.
Toll-like receptor (TLR) signaling induces a rapid reorganization of the actin cytoskeleton in cultured mouse dendritic cells (DC), leading to enhanced antigen endocytosis and a concomitant loss of filamentous actin-rich podosomes. We show that as podosomes are lost, TLR signaling induces prominent focal contacts and a transient reduction in DC ... More
Evolutionary modeling of combination treatment strategies to overcome resistance to tyrosine kinase inhibitors in non-small cell lung cancer.
AuthorsMumenthaler SM, Foo J, Leder K, Choi NC, Agus DB, Pao W, Mallick P, Michor F,
JournalMol Pharm
PubMed ID21995722
Many initially successful anticancer therapies lose effectiveness over time, and eventually, cancer cells acquire resistance to the therapy. Acquired resistance remains a major obstacle to improving remission rates and achieving prolonged disease-free survival. Consequently, novel approaches to overcome or prevent resistance are of significant clinical importance. There has been considerable ... More
Direct impact of T cells on neurons revealed by two-photon microscopy in living brain tissue.
AuthorsNitsch R, Pohl EE, Smorodchenko A, Infante-Duarte C, Aktas O, Zipp F
JournalJ Neurosci
PubMed ID15014121
Encephalitogenic T cells invade the brain during neuroinflammation such as multiple sclerosis (MS), inducing damage to myelin sheaths and oligodendrocytes. Only recently, neuronal structures were reported to be a crucial target in the disease. Here, two-photon microscopy using ion-sensitive dyes revealed that within the complex cellular network of living brain ... More
Induced recruitment of NK cells to lymph nodes provides IFN-gamma for T(H)1 priming.
AuthorsMartín-Fontecha A, Thomsen LL, Brett S, Gerard C, Lipp M, Lanzavecchia A, Sallusto F
JournalNat Immunol
PubMed ID15531883
Naive T cells are stimulated by antigen-presenting dendritic cells (DCs) in secondary lymphoid organs, but whether other types of cell participate in T cell priming is unclear. Here we show in mice that natural killer (NK) cells, which are normally excluded from lymph nodes, are rapidly recruited in a CCR7-independent, ... More
The strategy of T cell antigen-presenting cell encounter in antigen-draining lymph nodes revealed by imaging of initial T cell activation.
AuthorsBajénoff M, Granjeaud S, Guerder S
JournalJ Exp Med
PubMed ID12953093
The development of an immune response critically relies on the encounter of rare antigen (Ag)-specific T cells with dendritic cells (DCs) presenting the relevant Ag. How two rare cells find each other in the midst of irrelevant other cells in lymph nodes (LNs) is unknown. Here we show that initial ... More
Visualization and quantification of T cell-mediated cytotoxicity using cell-permeable fluorogenic caspase substrates.
AuthorsLiu L, Chahroudi A, Silvestri G, Wernett ME, Kaiser WJ, Safrit JT, Komoriya A, Altman JD, Packard BZ, Feinberg MB
JournalNat Med
PubMed ID11821904
We have developed a non-radioactive flow-cytometry assay to monitor and quantify the target-cell killing activities mediated by cytotoxic T lymphocytes (CTLs). This flow-cytometry CTL (FCC) assay is predicated on measurement of CTL-induced caspase activation in target cells through detection of the specific cleavage of fluorogenic caspase substrates. Here we show ... More
Detection and quantitation of cell-cell electrofusion products by flow cytometry.
AuthorsJaroszeski MJ, Gilbert R, Heller R
JournalAnal Biochem
PubMed ID7513970
A cytometric method for detecting and quantitating hybrid cells that resulted from cell-cell electrofusion was developed. Cells from two different lines and two vital fluorescent dyes were used in conjunction with a flow cytometer to demonstrate the method. Each dye was used to stain one cell type prior to electrofusion. ... More
Micromechanical control of cell-cell interactions.
AuthorsHui EE, Bhatia SN
JournalProc Natl Acad Sci U S A
PubMed ID17389399
The development and function of living tissues depends largely on interactions between cells that can vary in both time and space; however, temporal control of cell-cell interaction is experimentally challenging. By using a micromachined silicon substrate with moving parts, we demonstrate the dynamic regulation of cell-cell interactions via direct manipulation ... More
Selective staining by vital dyes of Müller glial cells in retinal wholemounts.
AuthorsUckermann O, Iandiev I, Francke M, Franze K, Grosche J, Wolf S, Kohen L, Wiedemann P, Reichenbach A, Bringmann A
JournalGlia
PubMed ID14648546
Müller glial cells within the retina may respond to different signaling molecules with an elevation of their intracellular free calcium. To prove the localization of the recorded calcium responses in Müller cells within acutely isolated retinal wholemounts, retinal pieces from adult animals and humans were exposed to different vital dyes ... More
Time-resolved polarization imaging by pump-probe (stimulated emission) fluorescence microscopy.
AuthorsBuehler C, Dong CY, So PT, French T, Gratton E
JournalBiophys J
PubMed ID10866979
We report the application of pump-probe fluorescence microscopy in time-resolved polarization imaging. We derived the equations governing the pump-probe stimulated emission process and characterized the pump and probe laser power levels for signal saturation. Our emphasis is to use this novel methodology to image polarization properties of fluorophores across entire ... More
Contacts and cooperation between cells depend on the hormone ouabain.
AuthorsLarre I, Ponce A, Fiorentino R, Shoshani L, Contreras RG, Cereijido M
JournalProc Natl Acad Sci U S A
PubMed ID16835298
Cell adhesion is a crucial step in proliferation, differentiation, migration, apoptosis, and metastasis. In previous works we have shown that cell adhesion is modulated by ouabain, a highly specific inhibitor of Na+,K+-ATPase, recently found to be a hormone. In the present work we pursue the investigation of the effect of ... More
MHC class II deprivation impairs CD4 T cell motility and responsiveness to antigen-bearing dendritic cells in vivo.
AuthorsFischer UB, Jacovetty EL, Medeiros RB, Goudy BD, Zell T, Swanson JB, Lorenz E, Shimizu Y, Miller MJ, Khoruts A, Ingulli E
JournalProc Natl Acad Sci U S A
PubMed ID17435166
The role continuous contact with self-peptide/MHC molecules (self ligands) in the periphery plays in the function of mature T cells remains unclear. Here, we elucidate a role for MHC class II molecules in T cell trafficking and antigen responsiveness in vivo. We find that naïve CD4 T cells deprived of ... More
Appetizing rancidity of apoptotic cells for macrophages: oxidation, externalization, and recognition of phosphatidylserine.
AuthorsKagan VE, Borisenko GG, Serinkan BF, Tyurina YY, Tyurin VA, Jiang J, Liu SX, Shvedova AA, Fabisiak JP, Uthaisang W, Fadeel B
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID12788785
Programmed cell death (apoptosis) functions as a mechanism to eliminate unwanted or irreparably damaged cells ultimately leading to their orderly phagocytosis in the absence of calamitous inflammatory responses. Recent studies have demonstrated that the generation of free radical intermediates and subsequent oxidative stress are implicated as part of the apoptotic ... More
GAS6 mediates adhesion of cells expressing the receptor tyrosine kinase Axl.
AuthorsMcCloskey P, Fridell YW, Attar E, Villa J, Jin Y, Varnum B, Liu ET
JournalJ Biol Chem
PubMed ID9287338
Axl is a receptor tyrosine kinase that contains both immunoglobulin and fibronectin III repeats in its extracellular domain reminiscent of cell adhesion molecules. Expression of the receptor tyrosine kinase Axl in the 32D myeloid cell line permits aggregation of cells in response to treatment with the native ligand GAS6; this ... More
PI(3)Kgamma has an important context-dependent role in neutrophil chemokinesis.
AuthorsFerguson GJ, Milne L, Kulkarni S, Sasaki T, Walker S, Andrews S, Crabbe T, Finan P, Jones G, Jackson S, Camps M, Rommel C, Wymann M, Hirsch E, Hawkins P, Stephens L
JournalNat Cell Biol
PubMed ID17173040
The directional movement of cells in a gradient of external stimulus is termed chemotaxis and is important in many aspects of development and differentiated cell function. Phophoinositide 3-kinases (PI(3)Ks) are thought to have critical roles within the gradient-sensing machinery of a variety of highly motile cells, such as mammalian phagocytes, ... More
Mechanically facilitated cell-cell electrofusion.
AuthorsJaroszeski MJ, Gilbert R, Fallon PG, Heller R
JournalBiophys J
PubMed ID7819489
Apparatus and methods were developed to enable mechanically facilitated cell-cell electrofusion to be performed. The apparatus and methods mechanically place cells in contact before fusion. The key component of this fusion system was a newly developed fusion chamber. The chamber was composed of two functionally identical electrodes that were housed ... More
Subset-specific regulation of the lymphatic exit of recirculating lymphocytes in vivo.
AuthorsYoung AJ, Marston WL, Dudler L
JournalJ Immunol
PubMed ID10975831
The blood-to-lymph recirculation of lymphocytes is required for the maintenance of immune surveillance and the dissemination of memory. Although the ability of lymph-borne cells to recirculate has been well documented, relatively less is known about the migration capacity of PBLs. We have found a clear preference for PBLs to recirculate ... More
Measuring hemodynamic changes during mammalian development.
AuthorsJones EA, Baron MH, Fraser SE, Dickinson ME
JournalAm J Physiol Heart Circ Physiol
PubMed ID15155254
The pathogenesis of many congenital cardiovascular diseases involves abnormal flow within the embryonic vasculature that results either from malformations of the heart or defects in the vasculature itself. Extensive genetic and genomic analysis in mice has led to the identification of an array of mutations that result in cardiovascular defects ... More
Mechanism of homophilic binding mediated by ninjurin, a novel widely expressed adhesion molecule.
Ninjurin is a novel protein that is up-regulated after nerve injury both in dorsal root ganglion (DRG) neurons and in Schwann cells. We previously reported that ninjurin demonstrates properties of a homophilic adhesion molecule and promotes neurite outgrowth from primary cultured DRG neurons. We have now found that ninjurin is ... More
Mucolipidosis IV consists of one complementation group.
AuthorsGoldin E, Cooney A, Kaneski CR, Brady RO, Schiffmann R
JournalProc Natl Acad Sci U S A
PubMed ID10411915
Mucolipidosis IV (MLIV) is an autosomal recessive disorder of unknown etiology characterized by severe visual impairment and psychomotor retardation. Recently, there has been considerable interest in positional cloning of the MLIV gene. It is unknown whether MLIV is a genetically homogenous disorder. In this paper, we present experiments that determined ... More
Constitutive death of platelets leading to scavenger receptor-mediated phagocytosis. A caspase-independent cell clearance program.
Apoptosis is a physiological program for the deletion of cells in which caspases govern events leading to safe clearance by phagocytes. However, a growing weight of evidence now suggests that not all forms of programmed cell death are caspase-dependent. We now report a complete and constitutive but caspase-independent program for ... More
HIV-1 gp41 six-helix bundle formation occurs rapidly after the engagement of gp120 by CXCR4 in the HIV-1 Env-mediated fusion process.
AuthorsGallo SA, Puri A, Blumenthal R
JournalBiochemistry
PubMed ID11591141
The onset of cell fusion mediated by HIV-1 IIIB Env is preceded by a lag phase of 15-20 min. Fusion mediated by the CD4-independent HIV-1 Env 8x, which is capable of interacting directly with CXCR4, proceeds with a greatly reduced lag phase. We probed the intermediate steps during the lag ... More
Optoinjection for efficient targeted delivery of a broad range of compounds and macromolecules into diverse cell types.
AuthorsClark IB, Hanania EG, Stevens J, Gallina M, Fieck A, Brandes R, Palsson BO, Koller MR
JournalJ Biomed Opt
PubMed ID16526911
Efficient delivery of compounds and macromolecules into living cells is essential in many fields including basic research, applied drug discovery, and clinical gene therapy. Unfortunately, current delivery methods, such as cationic lipids and electroporation, are limited by the types of macromolecules and cells that can be employed, poor efficiency, and/or ... More
MDA-MB-435 human breast carcinoma cell homo- and heterotypic adhesion under flow conditions is mediated in part by Thomsen-Friedenreich antigen-galectin-3 interactions.
The importance of Thomsen-Friedenreich antigen (T antigen)-galectin-3 interactions in adhesion of human breast carcinoma cells to the endothelium under conditions of flow was studied. Highly metastatic cells (MDA-MB-435) expressing high levels of both galectin-3 and T antigen demonstrated significantly increased adhesion to monolayers of endothelial cells compared with their non-metastatic ... More
Aldehyde fixation of thiol-reactive fluorescent cytoplasmic probes for tracking cell migration.
AuthorsWest CA, He C, Su M, Swanson SJ, Mentzer SJ
JournalJ Histochem Cytochem
PubMed ID11259454
Tracking of cell migration plays an important role in the study of morphogenesis, inflammation, and metastasis. The recent development of probes that exist as intracellular peptide-fluorescence dye adducts has offered the possibility of aldehyde fixation of these dyes for detailed anatomic studies of lymphocyte trafficking. To define the conditions for ... More
A dominant block to HIV-1 replication at reverse transcription in simian cells.
AuthorsMünk C, Brandt SM, Lucero G, Landau NR
JournalProc Natl Acad Sci U S A
PubMed ID12368468
Although nonhuman primates are genetically close to humans, their T cells do not support productive replication of HIV-1. In contrast, HIV-1 replicates in activated human CD4(+) T cells, monocytes, and metabolically active human cells of a variety of cell types become permissive for HIV-1 replication when transduced to express CD4 ... More
Dendritic cell elimination as an assay of cytotoxic T lymphocyte activity in vivo.
AuthorsRitchie DS, Hermans IF, Lumsden JM, Scanga CB, Roberts JM, Yang J, Kemp RA, Ronchese F
JournalJ Immunol Methods
PubMed ID11121552
We show in this paper that the survival of antigen-loaded dendritic cells in vivo may be used as a sensitive readout of CTL activity. We have previously shown that dendritic cells labeled with the fluorescent dye CFSE and injected sub-cutaneously into mice migrate spontaneously to the draining lymph node where ... More
Site-specific mutations in HIV-1 gp41 reveal a correlation between HIV-1-mediated bystander apoptosis and fusion/hemifusion.
AuthorsGarg H, Joshi A, Freed EO, Blumenthal R
JournalJ Biol Chem
PubMed ID17416587
The loss of CD4(+) T cells in HIV-1 infections is hypothesized to be caused by apoptosis of bystander cells mediated by cell surface-expressed HIV-1 Env glycoprotein. However, the mechanism by which Env mediates this process remains controversial. Specifically, the role of HIV-1 gp120 binding to CD4 and CXCR4 versus the ... More
Fluorescent lipid probes in the study of viral membrane fusion.
AuthorsBlumenthal R, Gallo SA, Viard M, Raviv Y, Puri A
JournalChem Phys Lipids
PubMed ID12093534
Fluorescent lipid probes are widely used in the observation of viral membrane fusion, providing a sensitive method to study fusion mechanism(s). Due to the wealth of data concerning liposome fusion, a variety of fusion assays has been designed including fluorescent probe redistribution, fluorescence dequenching, fluorescence resonance energy transfer and photosensitized ... More
Subpallial origin of a population of projecting pioneer neurons during corticogenesis.
AuthorsMorante-Oria J, Carleton A, Ortino B, Kremer EJ, Fairén A, Lledo PM
JournalProc Natl Acad Sci U S A
PubMed ID14523241
Pyramidal neurons of the mammalian cerebral cortex are generated in the ventricular zone of the pallium whereas the subpallium provides the cortex with inhibitory interneurons. The marginal zone contains a subpial stream of migratory interneurons and two different classes of transient neurons, the pioneer neurons provided with corticofugal axons, and ... More
Fluid shear regulates the kinetics and molecular mechanisms of activation-dependent platelet binding to colon carcinoma cells.
AuthorsMcCarty OJ, Jadhav S, Burdick MM, Bell WR, Konstantopoulos K
JournalBiophys J
PubMed ID12124268
This study was undertaken to investigate the kinetics and molecular requirements of platelet binding to tumor cells in bulk suspensions subjected to a uniform linear shear field, using a human colon adenocarcinoma cell line (LS174T) as a model. The effects of shear rate (20-1000 s(-1)), shear exposure time (30-300 s), ... More
Importance of VEGF for breast cancer angiogenesis in vivo: implications from intravital microscopy of combination treatments with an anti-VEGF neutralizing monoclonal antibody and doxorubicin.
AuthorsBorgström P, Gold DP, Hillan KJ, Ferrara N
JournalAnticancer Res
PubMed ID10628376
In the present study, we evaluated the effects of a neutralizing anti-Vascular Endothelial Growth Factor (VEGF) mAb, A4.6.1(200 micrograms twice weekly, i.p.), on angiogenesis and growth of tumor spheroids of human breast cancer cell lines (MCF-7, ZR-75 and, SK-BR-3) in nude mice. Furthermore, we investigated if in the presence of ... More
Anti-D initially stimulates an Fc-dependent leukocyte oxidative burst and subsequently suppresses erythrophagocytosis via interleukin-1 receptor antagonist.
AuthorsCoopamah MD, Freedman J, Semple JW
JournalBlood
PubMed ID12829590
Previous results have demonstrated that anti-D therapy in children with chronic auto-immune thrombocytopenic purpura (AITP) induced a significant increase in several pro- and anti-inflammatory plasma cytokines within 2 hours of administration. To investigate the biologic basis of these early in vivo responses, we developed a flow cytometric assay to measure ... More
Lactosylceramide mediates tumor necrosis factor-alpha-induced intercellular adhesion molecule-1 (ICAM-1) expression and the adhesion of neutrophil in human umbilical vein endothelial cells.
AuthorsBhunia AK, Arai T, Bulkley G, Chatterjee S
JournalJ Biol Chem
PubMed ID9852101
The endothelial expression of adhesion molecules by proinflammatory cytokines such as tumor necrosis factor-alpha (TNF-alpha) has been suggested to contribute to the initiation of atherosclerotic plaque formation. Since lactosylceramide (LacCer) accumulates in large quantities in human atherosclerotic plaque, we have explored its role in TNF-alpha-induced expression of intercellular adhesion molecule-1 ... More
Membrane-bound proteindisulfide isomerase (PDI) is involved in regulation of surface expression of thiols and drug sensitivity of B-CLL cells.
AuthorsTäger M, Kröning H, Thiel U, Ansorge S
JournalExp Hematol
PubMed ID9216735
The proteindisulfide isomerase (PDI), a multifunctional cytoplasmic enzyme with additional chaperone activity, has been shown recently, using monoclonal antibodies, to be located on the membrane of mature human B lymphocytes and B cell chronic lymphocytic leukemia (B-CLL) cells. Here, evidence is presented that this antigen exhibits catalytic activity as measured ... More
Human cytomegalovirus replicates abortively in polymorphonuclear leukocytes after transfer from infected endothelial cells via transient microfusion events.
AuthorsGerna G, Percivalle E, Baldanti F, Sozzani S, Lanzarini P, Genini E, Lilleri D, Revello MG
JournalJ Virol
PubMed ID10823870
Using a recently developed model for in vitro generation of pp65-positive polymorphonuclear leukocytes (PMNLs), we demonstrated that PMNLs from immunocompetent subjects may harbor both infectious human cytomegalovirus (HCMV) and viral products (pp65, p72, DNA, and immediate-early [IE] and pp67 late mRNAs) as early as 60 min after coculture with human ... More
T cell differentiation/maturation of CD34+ stem cells from HIV-seropositive hemophiliacs in cultured thymic epithelial fragments.
AuthorsRuiz M, Roodman ST, Bouhasin JD, Knutsen AP
JournalStem Cells
PubMed ID8820959
The clinical manifestations of AIDS are predominantly due to the cellular and humoral immune dysfunction caused by HIV infection, and thymic dysplasia caused by HIV infection probably contributes to the T cell lymphopenia. In the present study, T cell differentiation and/or maturation was assessed when enriched CD34+ stem cells (SCs ... More
Intercellular signalling in Drosophila segment formation reconstructed in vitro.
AuthorsCumberledge S, Krasnow MA
JournalNature
PubMed ID8505983
Genetic studies show that intercellular signalling is involved in key steps in Drosophila melanogaster development, but it has not previously been possible to investigate these processes in simplified in vitro systems. Analysis of engrailed (en) and wingless (wg) and other segment polarity genes suggests that two or more intercellular signalling ... More
New tools for quantifying and visualizing adoptively transferred cells in recipient mice.
AuthorsMoeller F, Nielsen FC, Nielsen LB
JournalJ Immunol Methods
PubMed ID14604542
Adoptive transfer of donor cells in mice is widely used in research on the function and metabolism of lymphocytes. We have evaluated new approaches for quantifying and visualizing adopted cells in recipient mouse tissue. We injected spleen cells from male beta-galactosidase (LacZ) transgenic mice into female wild type mice and ... More
MMP inhibitors augment fibroblast adhesion through stabilization of focal adhesion contacts and up-regulation of cadherin function.
AuthorsHo AT, Voura EB, Soloway PD, Watson KL, Khokha R
JournalJ Biol Chem
PubMed ID11500488
Increased pericellular proteolysis due to an imbalance between MMPs (matrix metalloproteinases) and TIMPs (tissue inhibitors of metalloproteinases) promotes early stages of tumorigenesis. We have reported that TIMP-1 down-regulation confers tumorigenicity on immortal Swiss 3T3 fibroblasts. In pursuit of the mechanism involved in this transformation, we asked whether MMP inhibitors modulate ... More
Spatial organization of signal transduction molecules in the NK cell immune synapses during MHC class I-regulated noncytolytic and cytolytic interactions.
AuthorsVyas YM, Mehta KM, Morgan M, Maniar H, Butros L, Jung S, Burkhardt JK, Dupont B
JournalJ Immunol
PubMed ID11591760
The cytolytic activity of NK cells is tightly regulated by inhibitory receptors specific for MHC class I Ags. We have investigated the composition of signal transduction molecules in the supramolecular activation clusters in the MHC class I-regulated cytolytic and noncytolytic NK cell immune synapses. KIR2DL3-positive NK clones that are specifically ... More
Oxidant generation by single infected monocytes after short-term fluorescence labeling of a protozoan parasite.
AuthorsChang HK, Thalhofer C, Duerkop BA, Mehling JS, Verma S, Gollob KJ, Almeida R, Wilson ME
JournalInfect Immun
PubMed ID17118986
Leishmania spp. are intracellular protozoa residing in mononuclear phagocytes. Leishmania organisms are susceptible to microbicidal responses generated in response to phagocytosis. Assuming that both phagocyte and parasite populations are heterogeneous, it is advantageous to examine the response of individual cells phagocytosing living parasites. Because Leishmania spp. lose virulence during the ... More
Selective adhesion of cells from different telencephalic regions.
Authors
JournalNeuron
PubMed ID8785052
Three dimensional dental epithelial-mesenchymal constructs of predetermined size and shape for tooth regeneration.
Authors
JournalBiomaterials
PubMed ID20682455
Principles of signaling pathway modulation for enhancing human naive pluripotency induction.
Authors
JournalCell Stem Cell
PubMed ID33915080
Learned adaptive multiphoton illumination microscopy for large-scale immune response imaging.