Rhodamine phalloidin is a high-affinity F-actin probe conjugated to the red-orange fluorescent dye, tetramethylrhodamine (TRITC).
Also available as a room-temperature-stable, ready-to-use solution:
ActinRed 555 Ready Probes Reagent.
See other Ready Probes ready-to-use imaging reagents and accessories ›•
Selectively stains F-actin•
Excitiation/Emission: 540/565 nm•
Superior to antibody staining•
Optimal for fixed and permeabilized samples•
Very widely cited fluorescent phalloidin conjugateGet Superior Results in Actin Staining StudiesPhalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly
Amanita phalloides 'death cap' mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while TRITC provides red-orange fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Rhodamine phalloidin allows high-contrast discrimination of actin staining. Rhodamine phalloidin is one of the most commonly used fluorescent phalloidin conjugates in the literature, as evidenced by over 1,500 citations (maintained in our in-house database).
Use in Multiple ApplicationsRhodamine phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Rhodamine phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot™ nanocrystals, and Alexa Fluor™ conjugates including secondary antibodies. For research use only.
Not intended for human or animal therapeutic or diagnostic use.
Related Links•
Read about labeling F-Actin with phallotoxins.
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Create a Cellular Masterpiece with Alexa Fluor™ Phalloidin using the Cell Paint Tool.