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Invitrogen™
Rhodamine Phalloidin
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Description
Rhodamine phalloidin is a high-affinity F-actin probe conjugated to the red-orange fluorescent dye, tetramethylrhodamine (TRITC).• Selectively stains F-actin
• Excitiation/Emission: 540/565 nm
• Superior to antibody staining
• Optimal for fixed and permeabilized samples
• Very widely cited fluorescent phalloidin conjugate
Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while TRITC provides red-orange fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Rhodamine phalloidin allows high-contrast discrimination of actin staining. Rhodamine phalloidin is one of the most commonly used fluorescent phalloidin conjugates in the literature, as evidenced by over 1,500 citations (maintained in our in-house database).
Use in Multiple Applications
Rhodamine phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Rhodamine phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and Alexa Fluor® conjugates including secondary antibodies. For research use only.
Not intended for human or animal therapeutic or diagnostic use.
Related Links
• Read about labeling F-Actin with phallotoxins.
• Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.
For Research Use Only. Not for use in diagnostic procedures.
Figures
Rat embryonic sensory neuron. Rhodamine phalloidin.
Mouse cerebellum section visualized using a fluorescent Qdot® nanocrystal conjugate.
Actin filaments of chick heart fibroblasts stained with rhodamine phalloidin
Muntjac skin fibroblast labeled with rhodamine phalloidin.
Macrophages cultured on a polymer surface that have fused to form a foreign-body giant cell following treatment with interleukin-4.
Specifications
| Label or Dye: | TRITC (Tetramethylrhodamine Isothiocyanate) |
|---|---|
| Sub-Cellular Localization: | Actin, Cytoskeleton |
| Excitation⁄Emission (nm): | 540⁄565 |
| Product Size: | 300 units |
| Shipping Condition: | Room Temperature |
Contents & storage
Store in freezer (-5 to -30°C) and protect from light.
