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Invitrogen™
Rhodamine Phalloidin
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Description
Rhodamine phalloidin is a high-affinity F-actin probe conjugated to the red-orange fluorescent dye, tetramethylrhodamine (TRITC).• Selectively stains F-actin
• Excitiation/Emission: 540/565 nm
• Superior to antibody staining
• Optimal for fixed and permeabilized samples
• Very widely cited fluorescent phalloidin conjugate
Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while TRITC provides red-orange fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Rhodamine phalloidin allows high-contrast discrimination of actin staining. Rhodamine phalloidin is one of the most commonly used fluorescent phalloidin conjugates in the literature, as evidenced by over 1,500 citations (maintained in our in-house database).
Use in Multiple Applications
Rhodamine phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Rhodamine phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and Alexa Fluor® conjugates including secondary antibodies. For research use only.
Not intended for human or animal therapeutic or diagnostic use.
Related Links
• Read about labeling F-Actin with phallotoxins.
• Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.
For Research Use Only. Not for use in diagnostic procedures.
Figures
Rat embryonic sensory neuron. Rhodamine phalloidin.
Actin filaments of chick heart fibroblasts stained with rhodamine phalloidin
Macrophages cultured on a polymer surface that have fused to form a foreign-body giant cell following treatment with interleukin-4.
Mouse cerebellum section visualized using a fluorescent Qdot® nanocrystal conjugate.
Muntjac skin fibroblast labeled with rhodamine phalloidin.
Specifications
| Label or Dye: | TRITC (Tetramethylrhodamine Isothiocyanate) |
|---|---|
| Sub-Cellular Localization: | Actin, Cytoskeleton |
| Excitation⁄Emission (nm): | 540⁄565 |
| Product Size: | 300 units |
| Shipping Condition: | Room Temperature |
Contents & storage
Store in freezer (-5 to -30°C) and protect from light.
