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Rhodamine Phalloidin

Catalog number: R415

Molecular Probes™  Related applications: Cell Structure

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Description

Rhodamine phalloidin is a high-affinity F-actin probe conjugated to the red-orange fluorescent dye, tetramethylrhodamine (TRITC).

Selectively stains F-actin
Excitiation/Emission: 540/565 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples
Very widely cited fluorescent phalloidin conjugate

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while TRITC provides red-orange fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Rhodamine phalloidin allows high-contrast discrimination of actin staining. Rhodamine phalloidin is one of the most commonly used fluorescent phalloidin conjugates in the literature, as evidenced by over 1,500 citations (maintained in our in-house database).

Use in Multiple Applications
Rhodamine phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Rhodamine phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and Alexa Fluor® conjugates including secondary antibodies. For research use only.

Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Label or Dye: TRITC (Tetramethylrhodamine Isothiocyanate)
Sub-Cellular Localization: Cytoskeleton, Actin
Product Size: 300 units
Excitation⁄Emission (nm): 540⁄565
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.

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Manuals & protocols

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