Rhodamine Phalloidin

Description

Rhodamine phalloidin is a high-affinity F-actin probe conjugated to the red-orange fluorescent dye, tetramethylrhodamine (TRITC).

• Selectively stains F-actin
• Excitiation/Emission: 540/565 nm
• Superior to antibody staining
• Optimal for fixed and permeabilized samples
• Very widely cited fluorescent phalloidin conjugate

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while TRITC provides red-orange fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Rhodamine phalloidin allows high-contrast discrimination of actin staining. Rhodamine phalloidin is one of the most commonly used fluorescent phalloidin conjugates in the literature, as evidenced by over 1,500 citations (maintained in our in-house database).

Use in Multiple Applications
Rhodamine phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Rhodamine phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and Alexa Fluor® conjugates including secondary antibodies. For research use only.

Not intended for human or animal therapeutic or diagnostic use.

Related Links
• Read about labeling F-Actin with phallotoxins.
• Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

Figures

Specifications

Label or Dye:	TRITC (Tetramethylrhodamine Isothiocyanate)
Sub-Cellular Localization:	Actin, Cytoskeleton
Excitation⁄Emission (nm):	540⁄565
Product Size:	300 units
Shipping Condition:	Room Temperature

Contents & storage