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Invitrogen™

BCEF, AM (2',7'-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein, Acetoxymethyl Ester) Solution

Catalog number:  B3051

Related applications:

Cell Analysis

| Industrial & Applied Science

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Description

BCECF, AM is a cell-permeant, dual-excitation ratiometric pH indicator with a pKa of ~6.98 and is ideal for measuring changes in the cytosolic pH of most cells. Intracellular pH measurements with BCECF are made by determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at ~490 nm versus the emission intensity when excited at its isobestic point of ~440 nm.

Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration: 1 mg⁄ml
For Use With (Equipment): Fluorescence Microscope, Flow Cytometer, Microplate Reader
Cell Permeability: Cell-Permeant
Detection Method: Fluorescent
Indicator Properties: Fluorescent Dye-Based
Indicator: pH Indicator
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C).