Pacific Orange™ dye

Pacific Orange dye is a bright orange-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 400/551 nm. Conjugates of this dye are strongly fluorescent even at neutral pH.

Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm and 551 nm, respectively, facilitating three-color analysis.

We offer Pacific Orange dye conjugated to a variety of primary antibodies, secondary antibodies and streptavidin. In addition, reactive and protein labeling kits are available so that you can produce your own antibody conjugates or probes.

Pacific Orange dye dashboard


Initial brightness

 
 
 
 
 
Pacific Orange dye is an orange fluorophore used primarily for flow cytometry, optimally excited by the 405 nm violet laser line.  
   

Photostability in buffer

 
 
 
 
 

Pacific Orange dye products

We offer a broad range of Pacific Orange dye products including labeling kits, labeled antibodies, bioconjugates and more:

Pacific Orange™ dye

Fluorescence excitation and emission spectra of Pacific Orange dye.

Pacific Orange reactive dyes

Select dye-labeling chemistries for conjugation with the following reactive groups: 

Amine

Pacific Orange dye protein/antibody labeling kits

Select an optimized labeling kit for your target antibody or protein:

Target protein Kit Cat. No.
Site-specific antibody labeling
<1–20 µg mouse IgG1 Zenon Pacific Orange Mouse IgG1 Labeling Kit Z25256
Conventional antibody/protein labeling
100 µg antibody/protein Pacific Orange Antibody/Protein Labeling Kit P30014
1 mg antibody/protein Pacific Orange Antibody/Protein Labeling Kit P30016

Violet laser–compatible antibody conjugates

We offer Pacific Blue, Pacific Green and Pacific Orange dyes as well as Qdot® probes for use with the violet laser. In addition to primary and secondary antibody conjugates of these dyes, we’ve developed kits for cell proliferation, immunophenotyping, dead cell discrimination, apoptosis, and cell cycle analysis—all aimed at helping you maximize the data you can get from your violet laser–equipped flow cytometer.

Learn more about Cell Health and Viability Assays for Flow Cytometry

 

Excitation spectra (dotted lines) and emission spectra (solid lines) for Molecular Probes violet laser–excited Pacific Blue dye (blue), Pacific Green dye (green), and Pacific Orange dye (orange). The shaded area represents the emission bandpass filter (522/31) used for detection of the Pacific Green dye.

Resources

Molecular Probes Handbook

The significantly revised 11th Edition is the most complete fluorescence labeling and detection reference available. The Molecular Probes Handbook can help you achieve the best experimental results for your fluorescence labeling and detection experiments. View the handbook online or order your own copy.

Go to the Handbook online
Order your copy of the Handbook

Flow cytometry instrument controls and standards

Keep your flow cytometry running at peak efficiency with instrument set-up, calibration, and counting beads including alignment and linearity beads, compensation tools, and cell-counting beads.

Go to flow cytometry instrument controls

Cell stain tool

Stain your own cell by using our simulation tool to develop reproducible results with many of our signature fluorescent dyes. Develop your multiplexing strategy for antibody probes, reactive dyes, and counterstains to create your perfectly labeled fluorescent cell.

Go to the cell stain tool

Fluorescence SpectraViewer

Plot and compare spectra and check the spectral compatibility for common fluorophores including all of the fluorescent probes from Thermo Fisher Scientific. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.

Go to the Fluorescence SpectraViewer