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Invitrogen Pacific Orange is a bright orange-fluorescent dye optimally excited by the 405 nm line of the violet laser, with excitation/emission at 400/551 nm and used primarily in flow cytometry applications. Conjugates of this dye are strongly fluorescent even at neutral pH. Pacific Orange and Pacific Blue dye conjugates can be simultaneously excited at 405 nm and emit at 551 nm and 455 nm respectively, facilitating two-color analysis.
We offer Pacific Orange dye conjugated to a variety of primary antibodies, secondary antibodies and streptavidin. In addition, reactive dyes and protein labeling kits are available to help you produce your own antibody conjugates or probes.
Protein Labeling Reagents Selection Guide (NHS ester, maleimide, etc.) Search Pacific Orange primary antibodies
Additional products
Staining of mouse splenocytes with Pacific Orange conjugated antibody. C57BL/6 mouse splenocytes were stained with CD3e Monoclonal Antibody (145-2C11), eFluor 450 and 0.125 µg of Rat IgG2a kappa Isotype Control (eBR2a), Pacific Orange (left) or 0.125 µg of CD4 Monoclonal Antibody (RM4-5), Pacific Orange (right). Cells in the lymphocyte gate were used for analysis.
Violet laser–compatible antibody conjugates
We offer Pacific Blue and Pacific Orange dyes, as well as Qdot probes, for use with the violet laser. In addition to primary and secondary antibody conjugates of these dyes, we have developed kits for cell proliferation, immunophenotyping, dead cell discrimination, apoptosis, and cell cycle analysis—all aimed at helping you maximize the data you can get from your violet laser-equipped flow cytometer.
Additional resources
Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
Cy™ is a trademark or registered trademark of GE Healthcare.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.