Invitrogen™

Pacific Orange™ and Pacific Blue™ Antibody Labeling Kits

Catalog number: P30014
Invitrogen™

Pacific Orange™ and Pacific Blue™ Antibody Labeling Kits

Catalog number: P30014
Pacific Orange and Pacific Blue antibody labeling kits enable attachment of a violet light-excitable fluorescent label on antibodies (up to 100 μg per reaction). Each kit contains enough dye for five reactions.
 
Catalog Number
P30014
Unit Size
1 kit
Description
Pacific Orange™ Antibody Labeling Kit
Excitation/Emission
405/551 nm
Color
Pacific Orange
Price (USD)
Availability
-
Full specifications
Quantity
DescriptionPacific Orange™ Antibody Labeling Kit
Product TypeAntibody Labeling Kit
Quantity5 Reactions
Sufficient For5 Reactions
Detection MethodFluorescence, Fluorescence
Excitation/Emission405/551 nm
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LinePacific Orange™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyePacific Orange, Pacific Orange
ColorPacific Orange
Labeling TargetAntibodies, Proteins
Label TypePacific Dyes
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Description
Pacific Blue™ Antibody Labeling Kit
Excitation/Emission
410/455 nm
Color
Pacific Blue
Price (USD)
Availability
-
Full specifications
Quantity
DescriptionPacific Blue™ Antibody Labeling Kit
Product TypeAntibody Labeling Kit
Quantity5 Reactions
Detection MethodFluorescence
Excitation/Emission410/455 nm
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LinePacific Blue™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyePacific Blue
ColorPacific Blue
Labeling TargetAntibodies, Proteins
Label TypePacific Dyes
Unit Size1 kit
Showing 2 of 2
Catalog NumberSpecificationsUnit SizeDescriptionExcitation/EmissionColorPrice (USD)AvailabilityQuantity
P30014Full specifications
1 kitPacific Orange™ Antibody Labeling Kit405/551 nmPacific Orange
-
DescriptionPacific Orange™ Antibody Labeling Kit
Product TypeAntibody Labeling Kit
Quantity5 Reactions
Sufficient For5 Reactions
Detection MethodFluorescence, Fluorescence
Excitation/Emission405/551 nm
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LinePacific Orange™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyePacific Orange, Pacific Orange
ColorPacific Orange
Labeling TargetAntibodies, Proteins
Label TypePacific Dyes
Unit Size1 kit
P30013Full specifications
1 kitPacific Blue™ Antibody Labeling Kit410/455 nmPacific Blue
-
DescriptionPacific Blue™ Antibody Labeling Kit
Product TypeAntibody Labeling Kit
Quantity5 Reactions
Detection MethodFluorescence
Excitation/Emission410/455 nm
Labeling Scale100 μg
Shipping ConditionRoom Temperature
Product LinePacific Blue™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyePacific Blue
ColorPacific Blue
Labeling TargetAntibodies, Proteins
Label TypePacific Dyes
Unit Size1 kit
Showing 2 of 2

A primary antibody directly labeled with a fluorophore often produces lower background fluorescence and less nonspecific binding. Primary antibodies of the same isotype or derived from the same species can be multiplexed if they are directly labeled with compatible fluorophores. Using Pacific Blue and Pacific Orange dyes simultaneously allows you to excite both dyes at the same wavelength while their emissions are differentially distinguished.

Pacific Orange and Pacific Blue antibody labeling kits allow the labeling of small amounts of antibody with a violet light-excitable dye (up to 100 μg of antibody per reaction). Small amounts of other proteins (>40 kDa) can also be labeled using these kits.

Features of these antibody labeling kits
• Pacific Blue excitation and emission maxima: 410/455 nm
• Pacific Orange excitation and emission maxima: 405/551 nm
• Labeled proteins typically ready to use in 90 min. (∼15 min. hands-on time)
• Optimized for small-scale labeling of any protein >40 kDa
• Stabilizing proteins should be removed from the sample before labeling
• Purified using convenient spin filters
• Includes detailed instructions for determining degree of labeling (DOL)

These kits contain everything you need to perform five separate labeling reactions as well as to purify the resulting conjugates. Conjugates are ideal for multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISA, immunocytochemistry, indirect FISH, and more. We are ISO 13485:2000 certified.

For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

Pacific Orange™ Antibody Labeling Kit comes with 5 vials of reactive dye, sodium bicarbonate, purification resin, spin columns and collections tubes.

Store at 2-8°C, protected from light. Component A may be stored from -20 to 8°C.

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