Invitrogen Alexa Fluor 405 dye is a blue-fluorescent dye with excitation ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. Alexa Fluor 405 dye is pH-insensitive over a wide molar range, helping ensure that you get stable signals in live-cell imaging applications.
Alexa Fluor 405 conjugates exhibit an excitation maximum at 401 nm and emission maximum at 421 nm. It is particularly useful in multicolor applications, including flow cytometry and super-resolution microscopy using STORM.
For imaging low-abundance targets, such as neuronal tracing in fine outgrowths, an anti–Alexa Fluor 405 antibody is available for signal amplification. Using this anti-dye antibody in conjunction with standard enzyme-mediated immunohistochemical methods can allow you to generate a more permanent, fade-free signal for light microscopy.
We offer Alexa Fluor 405 dye conjugated to a variety of antibodies and peptides optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to enable you to generate your own antibody conjugates or probes.
Alexa Fluor 405 dye dashboard
|Alexa Fluor 405 dye provides versatility in the blue channel for imaging and is compatible with the violet laser for flow cytometry. It is extremely useful in stochastic optical reconstruction microscopy (STORM) where it is used as an activator. It is also used to label conjugates for SIM.|
Photostability in buffer
Photostability in antifade
Performance in SRM
Alexa Fluor 405 dye products
We offer a broad range of Alexa Fluor 405 products, including labeling kits, secondary antibodies, and bioconjugates:
- Super-resolution microscopy (SRM) applications
- Antibody labeling kits for direct detection
- Streptavidin conjugate for signal amplification
- Cadaverine conjugate for cell tracing
- Anti-dye antibodies for signal amplification
Alexa Fluor 405 conjugated to CD4, used to label 10,000 cells and optimized for signal-to-background ratio.
Alexa Fluor 405 reactive dyes
Alexa Fluor 405 protein/antibody labeling kits
Select an optimized labeling kit for your target antibody or protein:
|Target protein||Kit||Cat. No.|
|Site-specific antibody labeling|
|<1–20 µg mouse IgG1||Zenon Alexa Fluor 405 Mouse IgG1 Labeling Kit||Z25013|
|<1–20 µg mouse IgG2a||Zenon Alexa Fluor 405 Mouse IgG2a Labeling Kit||Z25113|
|<1–20 µg mouse IgG2b||Zenon Alexa Fluor 405 Mouse IgG2b Labeling Kit||Z25213|
|<1–20 µg rabbit IgG||Zenon Alexa Fluor 405 Rabbit IgG Labeling Kit||Z25313|
Alexa Fluor dye family
Illuminating research for over 15 years
Invitrogen Alexa Fluor dyes are among the most trusted fluorescent dyes available today. In addition to superior performance, you’ll get a technical support team experienced in problem-solving, >50,000 published references, application and experimental tips, and protocols to help with experimental planning. For an outstanding research experience, choose Alexa Fluor dyes.
- Learn more about the brightness of Alexa Fluor dyes
- Learn more about the photostability of Alexa Fluor dyes
- Read all about Alexa Fluor dyes in the Invitrogen Molecular Probes Handbook
Molecular Probes Handbook
The significantly revised 11th Edition is the most complete fluorescence labeling and detection reference available. The Molecular Probes Handbook can help you achieve the best experimental results for your fluorescence labeling and detection experiments. View the handbook online or order your own copy.
Antifade reagents suppress photobleaching and preserve the signals of your fluorescently labeled target molecules. They offer excellent compatibility with a multitude of dyes across the spectrum, making them especially valuable tools for multicolor applications. For added convenience, some formulations include the nuclear counterstain DAPI, eliminating the need for a separate counterstaining step.
Cell stain tool
Stain your own cell by using our simulation tool to develop reproducible results with many of our signature fluorescent dyes. Develop your multiplexing strategy for antibody probes, reactive dyes, and counterstains to create your perfectly labeled fluorescent cell.
Plot and compare spectra and check the spectral compatibility for common fluorophores including all of the fluorescent probes from Thermo Fisher Scientific. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
For Research Use Only. Not for use in diagnostic procedures.