Invitrogen Alexa Fluor 546 dye is a bright, orange-fluorescent dye that can be excited using the 488 nm or 532 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 546 dye is pH-insensitive over a wide molar range.
Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 546 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.
We offer Alexa Fluor 546 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.
|An alternative for tetramethylrhodamine or Cy3 dye, with higher initial intensity than Alexa Fluor 555 dye, but lower photostability in buffer or antifade.|
Photostability in buffer
Photostability in antifade
Common filter set
We offer a broad range of Alexa Fluor 546 products including protein and antibody labeling kits, secondary antibodies, bioconjugates, and more.
Learn more here:
The intermediate filaments in bovine pulmonary artery endothelial cells were visualized using our anti-desmin antibody, followed by Alexa Fluor 647 goat anti–mouse IgG antibody (purple). Endogenous biotin in the mitochondria was labeled with Alexa Fluor 546 streptavidin (red), and DNA in the cell was stained with DAPI (white).
Select dye-labeling chemistries for conjugation with the following reactive groups:
Select an optimized labeling kit for your target antibody or protein:
|Target protein||Kit||Cat. No.|
|Site-specific antibody labeling|
|<1–20 µg mouse IgG1||Zenon Alexa Fluor 546 Mouse IgG1 Labeling Kit||Z25004|
|<1–20 µg rabbit IgG||Zenon Alexa Fluor 546 Rabbit IgG Labeling Kit||Z25304|
|Conventional antibody/protein labeling|
|100 µg antibody/protein||Alexa Fluor 546 Antibody/Protein Labeling Kit||A20183|
|1 mg antibody/protein||Alexa Fluor 546 Antibody/Protein Labeling Kit||A10237|
Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Learn more about the:
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and is used under license.
For Research Use Only. Not for use in diagnostic procedures.