Alexa Fluor 546 dyeInvitrogen Alexa Fluor 546 dye is a bright, orange-fluorescent dye that can be excited using the 488 nm or 532 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 546 dye is pH-insensitive over a wide molar range.

Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 546 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

We offer Alexa Fluor 546 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.

Alexa Fluor 546 dye dashboard


Initial brightness
 
 
 
 
 
An alternative for tetramethylrhodamine or Cy3 dye, with higher initial intensity than Alexa Fluor 555 dye, but lower photostability in buffer or antifade. 
   

Photostability in buffer
 
 
 
 
 
  

Photostability in antifade
 
 
 
 
 
 
 488/532TRITC556573 
 
Laser line
Common filter set
Excitation max
Emission max		 

Alexa Fluor® 546 dye

The intermediate filaments in bovine pulmonary artery endothelial cells were visualized using our anti-desmin antibody, followed by Alexa Fluor 647 goat anti–mouse IgG antibody (purple). Endogenous biotin in the mitochondria was labeled with Alexa Fluor 546 streptavidin (red), and DNA in the cell was stained with DAPI (white).

Alexa Fluor 546 reactive dyes

Select dye-labeling chemistries for conjugation with the following reactive groups: 

AmineThiol

Alexa Fluor 546 protein/antibody labeling kits

Select an optimized labeling kit for your target antibody or protein:

Target proteinKitCat. No.
Site-specific antibody labeling
<1–20 µg mouse IgG1Zenon Alexa Fluor 546 Mouse IgG1 Labeling KitZ25004
<1–20 µg rabbit IgGZenon Alexa Fluor 546 Rabbit IgG Labeling KitZ25304
Conventional antibody/protein labeling
100 µg antibody/proteinAlexa Fluor 546 Antibody/Protein Labeling KitA20183
1 mg antibody/proteinAlexa Fluor 546 Antibody/Protein Labeling KitA10237
Alexa Fluor Dye stained cell

Alexa Fluor dye family

Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Learn more about the:

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and is used under license.

For Research Use Only. Not for use in diagnostic procedures.