Alexa Fluor 647 dye

Alexa Fluor® 647 dye Invitrogen Alexa Fluor 647 dye is a bright, far-red–fluorescent dye with excitation ideally suited for the 594 nm or 633 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 647 dye is pH-insensitive over a wide molar range.

Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 647 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection.

We offer Alexa Fluor 647 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.

Alexa Fluor 647 dye dashboard

Initial brightness		Ideal for imaging low-abundance targets, especially in fixed cells; commonly used with Invitrogen Alexa Fluor 350, 488, and 594 dyes for multiplexing. In addition, is extremely useful in stochastic optical reconstruction microscopy (STORM) where it is an exceptional reporter for both dSTORM and nSTORM. It is also used in two-photon excitation (TPE) microscopy .

Photostability in buffer

Photostability in antifade

Performance in SRM

	594/633	Cy5	650	665
	Laser line	Common filter set	Excitation max	Emission max

Alexa Fluor 647 products

We offer a broad range of Alexa Fluor 647 products including protein and antibody labeling kits, secondary antibodies, bioconjugates, and more:

See all Alexa Fluor 647 Primary Antibodies
See all Alexa Fluor 647 Secondary Antibodies

STORM image of spectrin labeled with Alexa Fluor 647 in a hippocampal neuron. Image courtesy of Xue Liang Zhu et. al. Institute of Biochemistry and Cell Biology, Chinese Academy of Science, Shanghai.

Alexa Fluor 647 reactive dyes

Select dye-labeling chemistries for conjugation with the following reactive groups:

Amine	Thiol	Aldehyde, ketone, carboxylate, or phosphate	Click reaction
NHS ester	Maleimide	Hydrazide	Azide Alkyne

Alexa Fluor 647 protein/antibody labeling kits

Select an optimized labeling kit for your target antibody or protein:

Target protein	Kit	Cat. No.
Site-specific antibody labeling
<1–20 µg mouse IgG1	Zenon Alexa Fluor 647 Mouse IgG1 Labeling Kit	Z25008
<1–20 µg mouse IgG2a	Zenon Alexa Fluor 647 Mouse IgG2a Labeling Kit	Z25108
<1–20 µg mouse IgG2b	Zenon Alexa Fluor 647 Mouse IgG2b Labeling Kit	Z25208
<1–20 µg human IgG	Zenon Alexa Fluor 647 Human IgG Labeling Kit	Z25408
<1–20 µg rabbit IgG	Zenon Alexa Fluor 647 Rabbit IgG Labeling Kit	Z25308
Conventional antibody/protein labeling
10–20 µg antibody	APEX Alexa Fluor 647 Antibody Labeling Kit	A10475
20–100 µg antibody/protein	Alexa Fluor 647 Microscale Antibody/Protein Labeling Kit	A30009
100 µg antibody/protein	Alexa Fluor 647 Antibody/Protein Labeling Kit	A20186
1 mg antibody/protein	Alexa Fluor 647 Antibody/Protein Labeling Kit	A20173