Alexa Fluor® 647 dyeInvitrogen Alexa Fluor 647 dye is a bright, far-red–fluorescent dye with excitation ideally suited for the 594 nm or 633 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 647 dye is pH-insensitive over a wide molar range.

Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 647 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection.

We offer Alexa Fluor 647 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.

Alexa Fluor 647 dye dashboard


Initial brightness
 
 
 
 
 
Ideal for imaging low-abundance targets, especially in fixed cells; commonly used with Invitrogen Alexa Fluor 350, 488, and 594 dyes for multiplexing. In addition, is extremely useful in stochastic optical reconstruction microscopy (STORM) where it is an exceptional reporter for both dSTORM and nSTORM. It is also used in two-photon excitation (TPE) microscopy .  
   

Photostability in buffer
 
 
 
 
 
   

Photostability in antifade
 
 
 
 
 
   

Performance in SRM
 
 
 
 
 
  594/633 Cy5 650 665  
 
Laser line
Common filter set
Excitation max


Emission max

  
storm-antibody-conjugates

STORM image of spectrin labeled with Alexa Fluor 647 in a hippocampal neuron. Image courtesy of Xue Liang Zhu et. al. Institute of Biochemistry and Cell Biology, Chinese Academy of Science, Shanghai.

Alexa Fluor 647 reactive dyes

Select dye-labeling chemistries for conjugation with the following reactive groups: 

Amine Thiol Aldehyde, ketone, carboxylate, or phosphate Click reaction

Alexa Fluor 647 protein/antibody labeling kits

Select an optimized labeling kit for your target antibody or protein:

Target protein Kit Cat. No.
Site-specific antibody labeling
<1–20 µg mouse IgG1 Zenon Alexa Fluor 647 Mouse IgG1 Labeling Kit Z25008
<1–20 µg mouse IgG2a Zenon Alexa Fluor 647 Mouse IgG2a Labeling Kit Z25108
<1–20 µg mouse IgG2b Zenon Alexa Fluor 647 Mouse IgG2b Labeling Kit Z25208
<1–20 µg human IgG Zenon Alexa Fluor 647 Human IgG Labeling Kit Z25408
<1–20 µg rabbit IgG Zenon Alexa Fluor 647 Rabbit IgG Labeling Kit Z25308
Conventional antibody/protein labeling
10–20 µg antibody APEX Alexa Fluor 647 Antibody Labeling Kit A10475
20–100 µg antibody/protein Alexa Fluor 647 Microscale Antibody/Protein Labeling Kit A30009
100 µg antibody/protein Alexa Fluor 647 Antibody/Protein Labeling Kit A20186
1 mg antibody/protein Alexa Fluor 647 Antibody/Protein Labeling Kit A20173

Alexa Fluor dye family

Illuminating research for over 15 years

Alexa Fluor® dyesInvitrogen Alexa Fluor dyes are among the most trusted fluorescent dyes available today. In addition to superior performance, you’ll get a technical support team experienced in problem-solving, >50,000 published references, application and experimental tips, and protocols to help with experimental planning. For an outstanding research experience, choose Alexa Fluor dyes.

 

For Research Use Only. Not for use in diagnostic procedures.