Pacific Blue dye
Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH.
Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm and 551 nm, respectively, facilitating three-color analysis.
We offer Pacific Blue dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dyes and protein labeling kits are available so that you can produce your own antibody conjugates or probes.
Pacific Blue dye dashboard
 Initial brightness |
The violet laser–excitable Pacific Blue dye is optimized for use in flow cytometry applications, but can also be used for high-abundance targets in fluorescence microscopy. | ||
 Photostability in buffer |
| 15 | 405 | DAPI | 410 | 455 | ||
 Stain index |
 Laser line |
 Common filter set |
 Excitation max |
 Emission max |
Pacific Blue dye products
We offer a broad range of Pacific Blue dye products including labeling kits, labeled antibodies, bioconjugates, and more.
Fluorescence excitation and emission spectra of Pacific Blue dye.
Pacific Blue reactive dyes
Select dye-labeling chemistries for conjugation with the following reactive groups:
Pacific Blue dye protein/antibody labeling kits
Select an optimized labeling kit for your target antibody or protein:
| Target protein | Kit | Cat. No. |
|---|---|---|
| Site-specific antibody labeling | ||
| <1–20 µg mouse IgG1 | Zenon Pacific Blue Mouse IgG1 Labeling Kit | Z25041 |
| <1–20 µg mouse IgG2a | Zenon Pacific Blue Mouse IgG2a Labeling Kit | Z25156 |
| Conventional antibody/protein labeling | ||
| 10–20 µg antibody | APEX Pacific Blue Antibody Labeling Kit | A10478 |
| 100 µg antibody/protein | Pacific Blue Antibody/Protein Labeling Kit | P30013 |
| 1 mg antibody/protein | Pacific Blue Antibody/Protein Labeling Kit | P30012 |
Violet laser–compatible antibody conjugates
We offer Pacific Blue, Pacific Green and Pacific Orange dyes as well as Invitrogen Qdot probes for use with the violet laser. In addition to primary and secondary antibody conjugates of these dyes, we’ve developed kits for cell proliferation, immunophenotyping, dead cell discrimination, apoptosis, and cell cycle analysis—all aimed at helping you maximize the data you can get from your violet laser–equipped flow cytometer.
- Learn more about Cell Health and Viability Assays for Flow Cytometry
Excitation spectra (dotted lines) and emission spectra (solid lines) for Invitrogen Molecular Probes violet laser–excited Pacific Blue dye (blue), Pacific Green dye (green), and Pacific Orange dye (orange). The shaded area represents the emission bandpass filter (522/31) used for detection of the Pacific Green dye.
Resources
Molecular Probes Handbook
The significantly revised 11th Edition is the most complete fluorescence labeling and detection reference available. The Molecular Probes Handbook can help you achieve the best experimental results for your fluorescence labeling and detection experiments. View the handbook online or order your own copy.
Flow cytometry instrument controls and standards
Keep your flow cytometry running at peak efficiency with instrument set-up, calibration, and counting beads including alignment and linearity beads, compensation tools, and cell-counting beads.
Cell stain tool
Stain your own cell by using our simulation tool to develop reproducible results with many of our signature fluorescent dyes. Develop your multiplexing strategy for antibody probes, reactive dyes, and counterstains to create your perfectly labeled fluorescent cell.
Fluorescence SpectraViewer
Plot and compare spectra and check the spectral compatibility for common fluorophores including all of the fluorescent probes from Thermo Fisher Scientific. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.
For Research Use Only. Not for use in diagnostic procedures.