Alexa Fluor 350 dyeInvitrogen Alexa Fluor 350 dye is a blue-fluorescent dye with moderate photostability and excitation that matches the 350 nm laser line. The brightness and photostability of blue dyes are best suited to direct imaging of high-abundance targets.

Blue-fluorescent probes provide high-contrast discrimination for quantitative imaging applications without compromising performance in the popular red and blue channels. Alexa Fluor 350 protein conjugates are optimally excited at ~346 nm and produce bright, blue fluorescence at a wavelength slightly shorter than that of AMCA or AMCA conjugates, reducing Alexa Fluor 350 dye’s spectral overlap with the emission of fluorescein or Alexa Fluor 488 dye.

We offer Alexa Fluor 350 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.

Alexa Fluor 350 dye dashboard


Initial brightness
 
 
 
 
 
The brightest dye available in the blue channel is best used for high-abundance targets like cytoskeleton markers. Commonly used with Alexa Fluor 488, 594, and 647 dyes for multiplexing. 
  

Photostability in buffer
 
 
 
 
 
  

Photostability in antifade
 
 
 
 
 
 350DAPI346442 
 
Laser line
Common filter set
Excitation max
Emission max		 
cell-images.par.27737.image.dat-alexa-350-s001898-jpg

Fixed and permeabilized muntjac skin fibroblasts were stained with Alexa Fluor 350 phalloidin, a Mouse anti–ATP Synthase, Subunit IF1 Monoclonal Antibody, an anti–α-tubulin antibody, and an anti–cdc6 peptide antibody. The Mouse anti–ATP Synthase Subunit IF1 Monoclonal Antibody was prelabeled with the Invitrogen Zenon Alexa Fluor 488 Mouse IgG1 Labeling Kit (green), the anti–a-tubulin antibody was prelabeled with the Zenon Alexa Fluor 568 Mouse IgG1 Labeling Kit (red), and the anti–cdc6 peptide antibody was prelabeled with the Zenon Alexa Fluor 647 Mouse IgG1 Labeling Kit (pink). The image was deconvolved using Huygens software Scientific Volume Imaging.

Alexa Fluor 350 reactive dyes

Select dye-labeling chemistries for conjugation with the following reactive groups: 

AmineThiolAldehyde, ketone, carboxylate, or phosphate

Alexa Fluor 350 protein/antibody labeling kits

Select an optimized labeling kit for your target antibody or protein:

Target proteinKitCat. No.
Site-specific antibody labeling
<1–20 µg mouse IgG1Zenon Alexa Fluor 350 Mouse IgG1 Labeling KitZ25000
<1–20 µg mouse IgG2aZenon Alexa Fluor 350 Mouse IgG2a Labeling KitZ25100
<1–20 µg mouse IgG2bZenon Alexa Fluor 350 Mouse IgG2b Labeling KitZ25200
<1–20 µg rabbit IgGZenon Alexa Fluor 350 Rabbit IgG Labeling KitZ25300
Conventional antibody/protein labeling
100 µg antibody/proteinAlexa Fluor 350 Antibody/Protein Labeling KitA20180
1 mg antibody/proteinAlexa Fluor 350 Antibody/Protein Labeling KitA10170
Alexa Fluor Dye stained cell

Alexa Fluor dye family

Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Learn more about the:

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and is used under license.

For Research Use Only. Not for use in diagnostic procedures.