Citações e referências (25)

Invitrogen™

Phalloidin Labeling Probes

Name: Phalloidin Labeling Probes
SKU: A22281
Price: 5.020.01 BRL

Achieve precise and reliable F-actin staining with fluorescent and biotinylated phalloidins. Phalloidin conjugates are widely used in imaging applications to selectively label F-actin in a variety of sample types including fixed and permeabilized cells, tissue sections, and cell-free experiments.

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Número do catálogo	Color	Excitation Wavelength Range	Dye Type
A22281	Blue	346/442 nm	Alexa Fluor™ 350
A30104	Violet	405/450 nm	Alexa Fluor™ Plus 405
A12379	Green	495/518 nm	Alexa Fluor™ 488
O7466	Green	496/520 nm	Oregon Green™ 488
F432	Green	496/516 nm	FITC (Fluorescein)
A22282	Yellow	531/554 nm	Alexa Fluor™ 532
R415	Red-orange	540/565 nm	TRITC
A22283	Orange	556/570 nm	Alexa Fluor™ 546
A34055	Orange	555/565 nm	Alexa Fluor™ 555
A30106	Orange	555/565 nm	Alexa Fluor Plus 555
B3475	Red	558/569 nm	BODIPY™
A12380	Orange-red	578/600 nm	Alexa Fluor™ 568
A12381	Red	581/609 nm	Alexa Fluor™ 594
T7471	Red	591/608 nm	Texas Red™
A22284	Far-red	632/647 nm	Alexa Fluor™ 633
A34054	Far-red	633/647 nm	Alexa Fluor™ 635
A22287	Far-red	650/668 nm	Alexa Fluor™ 647
A30107	Far-red	650/668 nm	Alexa Fluor Plus 647
A22285	Near-infrared	663/690 nm	Alexa Fluor™ 660
A22286	Near-infrared	679/702 nm	Alexa Fluor™ 680
A30105	Near-infrared	758/784 nm	Alexa Fluor™ Plus 750
B7474	None	None	Biotin-XX
P3457	None	None	Phalloidin (unlabeled)

23 opções

Número do catálogo A22281

Preço (BRL)

5.020,01

Each

Color:

Blue

Excitation Wavelength Range:

346/442 nm

Dye Type:

Alexa Fluor™ 350

Preço (BRL)

5.020,01

Each

Fluorescent and biotinylated phalloidins are water soluble and bind to filamentous actin (F-actin) with nanomolar affinity, making them convenient probes for labeling, identifying, and quantifying F-actin in cryopreserved tissue sections, fixed and permeabilized cells, and cell-free experiments. Phalloidin conjugates bind similarly to actin from various species, including plants and animals, enabling staining of the cytoskeleton in a wide range of samples.

A variety of phalloidin conjugates for filamentous (F-actin) staining are available, including fluorescent Alexa Fluor and Alexa Fluor Plus phalloidins, along with phalloidins conjugated to classic fluorescent dyes such as BODIPY, fluorescein, and rhodamine. Phalloidin staining is spectrally compatible with other fluorescent stains used in cellular analyses such as GFP/RFP, Qdot nanocrystals, and other Alexa Fluor conjugates and antibodies. Biotin‐XX Phalloidin can be used to visualize actin filaments via fluorescent streptavidin tags or standard enzyme-mediated avidin/streptavidin techniques such as in electron microscopy. Unlabeled phalloidin is available for use as a control in blocking F‐actin staining or in promoting polymerization.

Phalloidin conjugates bind to both large and small actin filaments with similar affinity in a 1:1 stoichiometry between phallotoxin and actin subunits. They do not bind G-actin monomers.

Alexa Fluor and Alexa Fluor Plus phalloidin conjugates for F-actin staining

Fluorescent Alexa Fluor dye conjugates of phalloidin are popular F-actin stains, offering color choices across the full spectral range. These phalloidin conjugates provide researchers with fluorescent probes that are superior in brightness and photostability compared to other spectrally similar conjugates.

Alexa Fluor Plus Phalloidin conjugates retain the same specificity for actin but offer 3-5 times greater sensitivity and brightness compared to the corresponding Alexa Fluor Phalloidin conjugate. This increased brightness is beneficial for challenging F-actin imaging, such as the super‐resolution microscopy methods SIM and STORM, and for reliable staining of actin stress fibers.

Features of phalloidin probes

High specificity—binds selectively to F-actin, which allows for precise labeling of actin filaments in fixed cells and cryopreserved tissues
Strong affinity—nanomolar binding affinity for F-actin, which ensures stable and reliable actin staining
Extensive fluorescent conjugate options—over twenty conjugated varieties of phalloidin
Compatibility with fixed samples—typically used with fixed cells and tissues, making them suitable for actin staining in detailed structural studies, immunofluorescence staining, and IHC applications
Multiplexing capability—the wide availability of phalloidin conjugates enables their use in combination with other fluorescent probes and antibodies for multiplex imaging. Biotinylated phalloidin can be made use of in downstream streptavidin steps.
Quantitative analysis—can be used for quantitative analysis of F-actin distribution and density within cells, aiding in the study of cytoskeletal dynamics. The unlabeled phalloidin can be titrated as a control.
Ease of use—staining is straightforward and quick
Excellent stability—exhibit good photostability, which is essential for prolonged imaging sessions and time-lapse studies
Wide applicability—used for a range of applications, including studying cell morphology, motility, and the effects of drugs on the actin cytoskeleton

For Research Use Only. Not for use in diagnostic procedures.

Especificações

ColorBlue

Dye TypeAlexa Fluor™ 350

Excitation Wavelength Range346/442 nm

For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Confocal Microscope, Compatible with DAPI filter set

Product LineAlexa Fluor

Quantity300 Units

Shipping ConditionRoom Temperature

Label TypeAlexa Fluor Dyes

Product TypePhalloidin

Sub Cellular LocalizationActin, Cytoskeleton

Unit SizeEach

Conteúdo e armazenamento

Store in freezer -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

Do you offer alternative package sizes for phalloidin or ReadyProbes reagents?

Alternative package sizes may be available through a custom request process. Contact Thermo Fisher Scientific customer support or your sales representative to discuss available options for the specific reagent and quantity needed.

Can I use conjugated phalloidin label probes on organoids?

Yes. Conjugated phalloidin labeling probes can be used with organoids when F-actin is accessible after fixation and permeabilization. Because organoids are thicker than monolayer cell cultures, protocol optimization may be needed, including permeabilization conditions, probe incubation time, and probe concentration.

Are conjugated phalloidin reagents antibodies?

No, Phalloidin is not an antibody. It is a bicyclic peptide toxin originally isolated from the Amanita phalloides mushroom and is used to bind and label F-actin in fixed and permeabilized samples.

Can I use methanol instead of DMSO to reconstitute the conjugated phalloidins from the lyophilized solids?

Yes, methanol can be used to reconstitute fluorescent phalloidin, but it creates a less concentrated stock solution than DMSO. Dissolve the vial contents in 1.5 mL of methanol to yield a 40X stock solution at a concentration of 200 assays/mL, which is equivalent to approximately 6.6 µM. One unit/assay of fluorescent phalloidins is equivalent to 5 µL of the methanolic stock solution. Methanol may be used as a stock-solution solvent, but methanol-containing fixatives are not recommended because they can disrupt F-actin.

How do I reconstitute fluorescent conjugated phalloidin probes to make a stock solution?

For fluorescent phalloidin label probes, prepare a DMSO stock solution by dissolving the vial contents in 150 µL anhydrous DMSO to make a 400X stock, with a concentration of approximately 66 µM or 2,000 assays per mL. Store between -25°C and -5°C, protected from light. One unit/assay of fluorescent phalloidins is equivalent to 0.5 µL of the DMSO stock solution.

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Citações e referências (25)

Citações e referências

Abstract

Grp1-associated scaffold protein (GRASP) is a regulator of the ADP ribosylation factor 6 (Arf6)-dependent membrane trafficking pathway.

Authors:Venkataraman A, Nevrivy DJ, Filtz TM, Leid M,

Journal:Cell Biol Int

PubMed ID:22931251

GRASP interacts with Grp1 (general receptor for phosphoinositides 1; cytohesin 3), which catalyses nucleotide exchange on and activation of Arf6 (ADP-ribosylation factor-6). Arf6 is a low-molecular-mass GTPase that regulates key aspects of endocytic recycling pathways. Overexpressed GRASP accumulated in the juxtanuclear ERC (endocytic recycling compartment). GRASP co-localized with a constitutively ... More

A small-molecule approach to studying invasive mechanisms of Toxoplasma gondii.

Authors:Carey KL, Westwood NJ, Mitchison TJ, Ward GE

Journal:Proc Natl Acad Sci U S A

PubMed ID:15123807

'Toxoplasma gondii is the most common protozoan parasite of humans. Infection with T. gondii can lead to life-threatening disease as a result of repeated cycles of host cell invasion, parasite replication, and host cell lysis. Relatively little is known about the invasive mechanisms of T. gondii and related parasites within ... More

Polar localization of virulence-related Esx-1 secretion in mycobacteria.

Authors:Carlsson F, Joshi SA, Rangell L, Brown EJ,

Journal:PLoS Pathog

PubMed ID:19180234

'The Esx-1 (type VII) secretion system is critical for virulence of both Mycobacterium tuberculosis and Mycobacterium marinum, and is highly conserved between the two species. Despite its importance, there has been no direct visualization of Esx-1 secretion until now. In M. marinum, we show that secretion of Mh3864, a novel ... More

Focal adhesion size controls tension-dependent recruitment of alpha-smooth muscle actin to stress fibers.

Authors:Goffin JM, Pittet P, Csucs G, Lussi JW, Meister JJ, Hinz B

Journal:J Cell Biol

PubMed ID:16401722

'Expression of alpha-smooth muscle actin (alpha-SMA) renders fibroblasts highly contractile and hallmarks myofibroblast differentiation. We identify alpha-SMA as a mechanosensitive protein that is recruited to stress fibers under high tension. Generation of this threshold tension requires the anchoring of stress fibers at sites of 8-30-microm-long "supermature" focal adhesions (suFAs), which ... More

Restriction of receptor movement alters cellular response: physical force sensing by EphA2.

Authors:Salaita K, Nair PM, Petit RS, Neve RM, Das D, Gray JW, Groves JT,

Journal:Science

PubMed ID:20223987

'Activation of the EphA2 receptor tyrosine kinase by ephrin-A1 ligands presented on apposed cell surfaces plays important roles in development and exhibits poorly understood functional alterations in cancer. We reconstituted this intermembrane signaling geometry between live EphA2-expressing human breast cancer cells and supported membranes displaying laterally mobile ephrin-A1. Receptor-ligand binding, ... More

View all Phalloidin Labeling Probes citations