Grp1-associated scaffold protein (GRASP) is a regulator of the ADP ribosylation factor 6 (Arf6)-dependent membrane trafficking pathway.
AuthorsVenkataraman A, Nevrivy DJ, Filtz TM, Leid M,
JournalCell Biol Int
PubMed ID22931251
GRASP interacts with Grp1 (general receptor for phosphoinositides 1; cytohesin 3), which catalyses nucleotide exchange on and activation of Arf6 (ADP-ribosylation factor-6). Arf6 is a low-molecular-mass GTPase that regulates key aspects of endocytic recycling pathways. Overexpressed GRASP accumulated in the juxtanuclear ERC (endocytic recycling compartment). GRASP co-localized with a constitutively ... More
A small-molecule approach to studying invasive mechanisms of Toxoplasma gondii.
AuthorsCarey KL, Westwood NJ, Mitchison TJ, Ward GE
JournalProc Natl Acad Sci U S A
PubMed ID15123807
'Toxoplasma gondii is the most common protozoan parasite of humans. Infection with T. gondii can lead to life-threatening disease as a result of repeated cycles of host cell invasion, parasite replication, and host cell lysis. Relatively little is known about the invasive mechanisms of T. gondii and related parasites within ... More
Polar localization of virulence-related Esx-1 secretion in mycobacteria.
AuthorsCarlsson F, Joshi SA, Rangell L, Brown EJ,
JournalPLoS Pathog
PubMed ID19180234
'The Esx-1 (type VII) secretion system is critical for virulence of both Mycobacterium tuberculosis and Mycobacterium marinum, and is highly conserved between the two species. Despite its importance, there has been no direct visualization of Esx-1 secretion until now. In M. marinum, we show that secretion of Mh3864, a novel ... More
Focal adhesion size controls tension-dependent recruitment of alpha-smooth muscle actin to stress fibers.
AuthorsGoffin JM, Pittet P, Csucs G, Lussi JW, Meister JJ, Hinz B
JournalJ Cell Biol
PubMed ID16401722
'Expression of alpha-smooth muscle actin (alpha-SMA) renders fibroblasts highly contractile and hallmarks myofibroblast differentiation. We identify alpha-SMA as a mechanosensitive protein that is recruited to stress fibers under high tension. Generation of this threshold tension requires the anchoring of stress fibers at sites of 8-30-microm-long "supermature" focal adhesions (suFAs), which ... More
Restriction of receptor movement alters cellular response: physical force sensing by EphA2.
AuthorsSalaita K, Nair PM, Petit RS, Neve RM, Das D, Gray JW, Groves JT,
JournalScience
PubMed ID20223987
'Activation of the EphA2 receptor tyrosine kinase by ephrin-A1 ligands presented on apposed cell surfaces plays important roles in development and exhibits poorly understood functional alterations in cancer. We reconstituted this intermembrane signaling geometry between live EphA2-expressing human breast cancer cells and supported membranes displaying laterally mobile ephrin-A1. Receptor-ligand binding, ... More
Changes in the balance between caldesmon regulated by p21-activated kinases and the Arp2/3 complex govern podosome formation.
AuthorsMorita T, Mayanagi T, Yoshio T, Sobue K
JournalJ Biol Chem
PubMed ID17224451
'Podosomes are dynamic cell adhesion structures that degrade the extracellular matrix, permitting extracellular matrix remodeling. Accumulating evidence suggests that actin and its associated proteins play a crucial role in podosome dynamics. Caldesmon is localized to the podosomes, and its expression is down-regulated in transformed and cancer cells. Here we studied ... More
A novel p21-activated kinase binds the actin and microtubule networks and induces microtubule stabilization.
AuthorsCau J, Faure S, Comps M, Delsert C, Morin N
JournalJ Cell Biol
PubMed ID11733543
'Coordination of the different cytoskeleton networks in the cell is of central importance for morphogenesis, organelle transport, and motility. The Rho family proteins are well characterized for their effects on the actin cytoskeleton, but increasing evidence indicates that they may also control microtubule (MT) dynamics. Here, we demonstrate that a ... More
Galpha12/13 is essential for directed cell migration and localized Rho-Dia1 function.
'Scratch-wound assays are frequently used to study directed cell migration, a process critical for embryogenesis, invasion, and tissue repair. The function and identity of trimeric G-proteins in cell behavior during wound healing is not known. Here we show that Galpha12/13, but not Galphaq/11 or Galphai, is indispensable for coordinated and ... More
Pseudomonas aeruginosa exploits a PIP3-dependent pathway to transform apical into basolateral membrane.
AuthorsKierbel A, Gassama-Diagne A, Rocha C, Radoshevich L, Olson J, Mostov K, Engel J
JournalJ Cell Biol
PubMed ID17403925
Pseudomonas aeruginosa, an important human pathogen, preferentially binds and enters injured cells from the basolateral (BL) surface. We previously demonstrated that activation of phosphatidylinositol 3-kinase (PI3K) and Akt are necessary and sufficient for P. aeruginosa entry from the apical (AP) surface and that AP addition of phosphatidylinositol 3,4,5-trisphosphate (PIP3) is ... More
Two distinct distributions of F-actin are present in the hyphal apex of the oomycete Achlya bisexualis.
AuthorsYu YP, Jackson SL, Garrill A
JournalPlant Cell Physiol
PubMed ID15047875
We show that two distinct distributions of F-actin are present in the hyphal apex of the oomycete Achlya bisexualis, that have been chemically fixed with a combination of methylglyoxal and formaldehyde and stained with Alexa phalloidin. In approximately one half of the hyphae examined, an F-actin depleted zone within the ... More
NIPA1(SPG6), the basis for autosomal dominant form of hereditary spastic paraplegia, encodes a functional Mg2+ transporter.
AuthorsGoytain A, Hines RM, El-Husseini A, Quamme GA
JournalJ Biol Chem
PubMed ID17166836
Mutations in the NIPA1(SPG6) gene, named for "nonimprinted in Prader-Willi/Angelman" has been implicated in one form of autosomal dominant hereditary spastic paraplegia (HSP), a neurodegenerative disorder characterized by progressive lower limb spasticity and weakness. However, the function of NIPA1 is unknown. Here, we show that reduced magnesium concentration enhances expression ... More
The type III intermediate filament vimentin regulates organelle distribution and modulates autophagy.
AuthorsBiskou O, Casanova V, Hooper KM, Kemp S, Wright GP, Satsangi J, Barlow PG, Stevens C
JournalPLoS One
PubMed ID30699149
'The cytoskeletal protein vimentin plays a key role in positioning of organelles within the cytosol and has been linked to the regulation of numerous cellular processes including autophagy, however, how vimentin regulates autophagy remains relatively unexplored. Here we report that inhibition of vimentin using the steroidal lactone Withaferin A (WFA) ... More
Multi-directional cellular alignment in 3D guided by electrohydrodynamically-printed microlattices.
AuthorsMao M, He J, Li Z, Han K, Li D
JournalActa Biomater
PubMed ID31669696
'Recapitulating aligned cellular architectures of native tissues in vitro is important to engineer artificial tissue analogs with desired biological functions. Here a novel strategy is presented to direct three-dimensional (3D) cellular alignment by embedding cell/collagen hydrogel into the predefined electrohydrodynamically-printed microlattices. The cell/collagen hydrogel, originally filled within the printed microlattices ... More
Inhibition of thyroid hormone signaling protects retinal pigment epithelium and photoreceptors from cell death in a mouse model of age-related macular degeneration.
AuthorsMa H, Yang F, Ding XQ
JournalCell Death Dis
PubMed ID31932580
'Age-related macular degeneration (AMD) is the leading cause of blindness in the elderly. Dry AMD is characterized by a progressive macular degeneration of the retinal pigment epithelium (RPE) and photoreceptors, and the RPE oxidative damage/dystrophy is at the core of the disease. Recent population/patients-based studies have shown an association of ... More
Using patterned supported lipid membranes to investigate the role of receptor organization in intercellular signaling.
Authors
JournalNat Protoc
PubMed ID21455188
RhoA drives actin compaction to restrict axon regeneration and astrocyte reactivity after CNS injury.
Authors
JournalNeuron
PubMed ID34508667
Myosin X Interaction with KIF13B, a Crucial Pathway for Netrin-1-Induced Axonal Development.
Authors
JournalJ Neurosci
PubMed ID33097641
Integrin-Mediated Adhesion in the Unicellular Holozoan Capsaspora owczarzaki.
Authors
JournalCurr Biol
PubMed ID32857975
Evaluation of Stable LifeAct-mRuby2- and LAMP1-NeonGreen Expressing A549 Cell Lines for Investigation of Aspergillus fumigatus Interaction with Pulmonary Cells.
Authors
JournalInt J Mol Sci
PubMed ID34073107
Epigenetic regulation of Smad2 and Smad3 by profilin-2 promotes lung cancer growth and metastasis.
Authors
JournalNat Commun
PubMed ID26354229
Cytotoxic Efficacy and Resistance Mechanism of a TRAIL and VEGFA-Peptide Fusion Protein in Colorectal Cancer Models.
Authors
JournalInt J Mol Sci
PubMed ID33808900
Ring finger protein 213 assembles into a sensor for ISGylated proteins with antimicrobial activity.
Authors
JournalNat Commun
PubMed ID34599178
Advanced maturation of human cardiac tissue grown from pluripotent stem cells.
Authors
JournalNature
PubMed ID29618819
Assessment of the Angiogenic Potential of 2-Deoxy-D-Ribose Using a Novel
AuthorsDikici S, Aldemir Dikici B, Bhaloo SI, Balcells M, Edelman ER, MacNeil S, Reilly GC, Sherborne C, Claeyssens F
JournalFront Bioeng Biotechnol
PubMed ID32010677
Angiogenesis is a highly ordered physiological process regulated by the interaction of endothelial cells with an extensive variety of growth factors, extracellular matrix components and mechanical stimuli. One of the most important challenges in tissue engineering is the rapid neovascularization of constructs to ensure their survival after transplantation. To achieve ... More
Tks5 SH3 domains exhibit differential effects on invadopodia development.
AuthorsDaly C, Logan B, Breeyear J, Whitaker K, Ahmed M, Seals DF
JournalPLoS One
PubMed ID31999741
The Src substrate Tks5 helps scaffold matrix-remodeling invadopodia in invasive cancer cells. Focus was directed here on how the five SH3 domains of Tks5 impact that activity. Mutations designed to inhibit protein-protein interactions were created in the individual SH3 domains of Tks5, and the constructs were introduced into the LNCaP ... More