Figure 1. Spectral signature of Alexa Fluor 561.

Alexa Fluor 561 is a bright dye optimized for flow cytometers with spectral capabilities. This fluorophore can be used for cell surface, intracellular, and intranuclear antigens. It should be used in complex multicolor panels to fit between fluorophores FITC and PE. In simpler panels, Alexa Fluor 561 can replace PE to reduce emission spreading into PE tandem dyes.


Alexa Fluor 561 dye dashboard


Initial brightness
 
 
 
 
 
The yellow laser–excitable Alexa Fluor 561 dye is optimized for use in spectral flow cytometry applications. 
  

Photostability in buffer
 
 
 
 
 

 561558575 
 
Laser line
Excitation max


Emission max

 


Alexa Fluor 561 dye products

We offer Alexa Fluor 561 dye conjugated to primary antibodies for use in flow cytometry.

Learn more here:

Figure 2. CD4-Alexa Fluor 561 staining. Normal human peripheral blood cells were stained with CD3 Monoclonal Antibody, FITC (Cat. No. 11-0037-42) and Mouse IgG1 kappa Isotype Control, Alexa Fluor 561 (Cat. No. 505-4714-81) (left) or CD4 Monoclonal Antibody, Alexa Fluor 561 (right). Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD (Cat. No. 00-6993-50). This data was collected on a 5-laser Cytek Aurora full spectral cytometer.

Figure 3. Alexa Fluor 561 non-specific staining in SJL mice. Balb/c mouse splenocytes (left) and SJL mouse splenocytes (right) were stained with CD19 Monoclonal Antibody, FITC and human specific Monoclonal Antibody, Alexa Fluor 561. This experiment shows that Alexa Fluor 561 non-specifically stains B cells in SJL mice (and Swiss Webster, data not shown). No non-specific staining has been observed in Balb/c mice (and C57BL/6, data not shown).

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Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and is used under license.

For Research Use Only. Not for use in diagnostic procedures.