Invitrogen Cy5 dye is a bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 dye or Invitrogen Alexa Fluor 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.
The Cy5 fluorophore is also the basis for the lipophilic neuronal and long-term DiD cell tracing reagents, which append alkyl tails (≥12 carbons) onto the core fluorophore.
For stable signal generation, Alexa Fluor 647 dye is generally brighter than Cy5 dye in protein and nucleic acid conjugates. Allophycocyanin (APC) is another bright fluorophore that is spectrally equivalent to Cy5 dye and Alexa Fluor 647 dye, but it is used almost exclusively in flow cytometry.
|Cy5 dye is a traditional far-red–fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications. It is also the basis for the classic lipophilic tracer DiD and its variants.|
Photostability in buffer
Photostability in antifade
We offer a range of Cy5 dye and DiD products including secondary antibodies, streptavidin conjugates, kits, and more. Learn more here:
- Streptavidin conjugates for signal amplification
- Neuronal tracers
- Cell tracers
- Nucleic acid labeling
- RNA amplification kits
Learn more about fluorescent dyes in the Cy5 wavelength range
High-performance alternatives to Cy5 dye
We offer Alexa Fluor 647 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.
Learn more about Alexa Fluor 647 dye
Allophycocyanin, also known as APC, is typically used for flow cytometry, microarrays, ELISA, and other applications that require either high sensitivity or simultaneous multicolor detection. We offer APC conjugated to a variety of primary and secondary antibodies for flow cytometry as well as tandem dye constructs for longer Stokes shift detection.
5 steps resources
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
For Research Use Only. Not for use in diagnostic procedures.