Qdot 655 probe is an excellent far red–fluorescent label with outstanding brightness and photostability for imaging and flow cytometry applications. With an emission maximum of ~655 nm, Qdot 655 probe can be excited using a 350, 488, 595, or 615 nm laser line or standard Cy5 filter. Optimum imaging results with brighter signals are achieved with a Qdot 655 filter or light cube.
Made from nanocrystals of semiconductor material, Qdot probes are broadly compatible with standard fluorescence techniques, but there are some novel aspects to their use that can be optimized to obtain the best possible results. In general they have an extremely broad excitation range and narrow emission with a large Stokes shift. Optimal brightness is generally achieved using a specific Qdot filter.
Organic fluorophores and Qdot probes can be readily combined in multiplexed flow cytometry or imaging studies. Longer-wavelength Qdot probes are also ideal for multiplexed western blots.
Qdot 655 probe dashboard
 Initial brightness |
For best results use a Qdot-optimized filter and PBS or a mounting medium without antifade.
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 Photostability in buffer |
| 20 | 350, 488, 595, 615 | Cy5 | UV | 655 |
 Stain index |
 Laser line |
 Common filter set |
 Excitation max |
 Emission max |
Qdot 655 products
The exceptional brightness and photostability of Qdot probes make it easier to detect low-abundance targets. With narrow and symmetric emission spectra, Qdot probes are also ideal for multiplexed fluorescence detection using a combination of Qdot probes and organic dyes.
- Qdot 655 streptavidin
- Biotin-labeled Qdot 655 probes
- Qdot 655 primary antibody conjugates
- Qdot 655 secondary antibody conjugates
- Isotype antibody controls—Mouse IgG2a, Qdot 655 conjugate
- Qdot 655 Wheat Germ Agglutinin Conjugate
- SiteClick Qdot 655 Antibody Labeling Kit
- Qtracker 655 Cell Labeling Kit
- Qtracker 655 Vascular Labels
BPAE cells were fixed and permeabilized using the Image-iT Fixation/Permeabilization Kit and incubated with Anti- ATP Synthase Subunit IF1 followed by GAM-QD655. Cells were labeled with ReadyProbes reagents NucBlue Live and ActinGreen 488, then mounted using ProLong Gold Antifade Reagent. Images were taken using the EVOS FL Auto Imaging System and a 40X CoverSlip corrected Objective.
Qdot 655 conjugation kits
We’ve developed functionalized versions of the Qdot 655 label for the preparation of custom conjugates of proteins and other biomolecules. The first—a carboxyl-derivatized form—can be coupled to amine groups of proteins and modified oligonucleotides. The second—an amine-derivatized PEG form—can be coupled to isothiocyanates and succinimidyl esters (or with native carboxylic acids using water-soluble carbodiimides).
| Amine | Aldehyde-, ketone, carboxylate or phosphate |
|---|---|
Qdot probes and accessories
Qdot Probes are made from nanocrystals of semiconductor material and are broadly compatible with standard fluorescence techniques, but there are some novel aspects to their use that can be optimized to obtain the best possible results. Learn more about Qdot Probes:
- Qdot probes overview
- Qdot probe technology
- Qdot probes structure
- Qdot probes size
- EVOS Light Cube, Qdot 565
Designed to eliminate the complexities of microscopy without compromising performance. The Qdot 565 light cube is ideal for use with Qdot 565 and other fluorophores with excitation and emission maxima near 445 and 565 nm, respectively. - See a complete list of Qdot products
Resources
Molecular Probes Handbook
The significantly revised 11th Edition is the most complete fluorescence labeling and detection reference available. The Molecular Probes Handbook can help you achieve the best experimental results for your fluorescence labeling and detection experiments. View the handbook online or order your own copy.
Antifades and mounting media
High-performance antifade reagents like ProLong Antifade and SlowFade Antifade will inhibit the photobleaching of organic dyes but suppress the fluorescence of Qdot probes. A better solution for imaging Qdot probes is simply PBS or a clear, colorless toluene-based mounting medium formulated from acrylic resins without the addition of antifade components.
Cell stain tool
Stain your own cell by using our simulation tool to develop reproducible results with many of our signature fluorescent dyes. Develop your multiplexing strategy for antibody probes, reactive dyes, and counterstains to create your perfectly labeled fluorescent cell.
Fluorescence SpectraViewer
Plot and compare spectra and check the spectral compatibility for common fluorophores including all of the fluorescent probes from Thermo Fisher Scientific. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
For Research Use Only. Not for use in diagnostic procedures.
