Qdot® 655 Probe
Qdot® 655 probe is an excellent far red–fluorescent label with outstanding brightness and photostability for imaging and flow cytometry applications. With an emission maximum of ~655 nm, Qdot® 655 probe can be excited using a 350, 488, 595, or 615 nm laser line or standard Cy®5 filter. Optimum imaging results with brighter signals are achieved with a Qdot® 655 filter or light cube.
Made from nanocrystals of semiconductor material, Qdot® probes are broadly compatible with standard fluorescence techniques, but there are some novel aspects to their use that can be optimized to obtain the best possible results. In general they have an extremely broad excitation range and narrow emission with a large Stokes shift. Optimal brightness is generally achieved using a specific Qdot® filter.
Organic fluorophores and Qdot® probes can be readily combined in multiplexed flow cytometry or imaging studies. Longer-wavelength Qdot® probes are also ideal for multiplexed western blots.
Qdot® 655 products
The exceptional brightness and photostability of Qdot® probes make it easier to detect low-abundance targets. With narrow and symmetric emission spectra, Qdot® probes are also ideal for multiplexed fluorescence detection using a combination of Qdot® probes and organic dyes.
- Qdot® 655 streptavidin
- Biotin-labeled Qdot® 655 probes
- Qdot® 655 secondary antibody conjugates
- Qdot® 655 primary antibody conjugates
- Isotype antibody controls—Mouse IgG2a, Qdot® 655 conjugate
- Qdot® 655 Goat Anti-Fluorescein Conjugate, whole IgG
- Qdot® 655 Wheat Germ Agglutinin Conjugate
- Antibody labeling—Qdot® 655 Antibody Conjugation Kit and the SiteClick™ Qdot® 655 Antibody Labeling Kit
- Qtracker® 655 Cell Labeling Kit
- Qtracker® 655 Vascular Labels
BPAE cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit and incubated with Anti- ATP Synthase Subunit IF1 followed by GAM-QD655. Cells were labeled with ReadyProbes reagents NucBlue® Live and ActinGreen™ 488, then mounted using ProLong® Gold Antifade Reagent. Images were taken using the EVOS® FL Auto Imaging System and a 40X CoverSlip corrected Objective.
Qdot® 655 conjugation kits
We’ve developed functionalized versions of the Qdot® 655 label for the preparation of custom conjugates of proteins and other biomolecules. The first—a carboxyl-derivatized form—can be coupled to amine groups of proteins and modified oligonucleotides. The second—an amine-derivatized PEG form—can be coupled to isothiocyanates and succinimidyl esters (or with native carboxylic acids using water-soluble carbodiimides).
|Amine||Aldehyde-, ketone, carboxylate or phosphate|
Qdot® probes and accessories
Qdot® Probes are made from nanocrystals of semiconductor material and are broadly compatible with standard fluorescence techniques, but there are some novel aspects to their use that can be optimized to obtain the best possible results. Learn more about Qdot® Probes:
- Qdot® probes overview
- Qdot® probe technology
- Qdot® probes structure
- Qdot® probes size
- EVOS® Light Cube, Qdot® 565
Designed to eliminate the complexities of microscopy without compromising performance. The Qdot® 565 light cube is ideal for use with Qdot® 565 and other fluorophores with excitation and emission maxima near 445 and 565 nm, respectively.
- See a complete list of Qdot® products