graph of emission spectra for PE-eFluor 610 dye

The PE-eFluor 610 dye is a high-performance tandem fluorophore designed for use with flow cytometers equipped with blue (488 nm), green (532 nm), or yellow-green (561 nm) laser. It has a peak emission of 607 nm that can be detected using typical filters for PE-Texas Red dye, such as a 610/20 bandpass filter.

Benefits of PE-eFluor 610 include

  • Brighter than PE-Texas Red dye antibody conjugates
  • Reduced compensation out of other far-red channels
  • Stable performance when treated with formaldehyde fixation or exposed to ambient light

PE-eFluor 610 dye dashboard


Initial brightness

 
 
 
 
 
PE-eFluor 610 dye is a tandem fluorophore developed for use in multicolor flow cytometry.  
   

Photostability in buffer

 
 
 
 
 

  488/532/561 610/20 532-561 607  
 
Laser line

Common filter set

Excitation max

Emission max
 
Flow histogram comparing PE-eFluor 610 dye to PE-Texas Red dye

PE-eFluor 610 dye is brighter than PE-Texas Red dye. Normal human peripheral blood cells were stained with CD3 conjugated to PE-eFluor 610 dye (clone UCHT1) (dark orange line) or PE-Texas Red dye (light orange line).

Flow histogram showing fluorescence intensity of PE-eFluor 610 dye before and after extended ambient light exposure

Photostability of PE-eFluor 610 dye. Normal human peripheral blood cells were stained with Anti-CD3 PE-eFluor 610 (clone UCHT1). Cells were then washed and incubated for six hours at 4°C in the dark (red line), at room temperature in ambient light (blue line), or at room temperature on the windowsill (orange line).

PE-eFluor 610 dye products

We offer PE-eFluor 610 dye conjugated to primary antibodies for use in flow cytometry.

Learn more here:

See all PE-eFluor 610 Primary Antibodies
See all PE-eFluor 610 Secondary Antibodies

Resources

Primary antibodies to match your cytometer's lasers

Cell stain tool

To support your multicolor flow cytometry experiments, our antibody portfolios include a variety of dyes with compatibility to all commonly used flow cytometry lasers.

Fluorescence SpectraViewer

thumbnail50x50.par.42353.image.50.50.1.thu-spectraviewer-jpg

Plot and compare spectra and check the spectral compatibility for common fluorophores including all of the fluorescent probes from Thermo Fisher Scientific. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.

Go to the Fluorescence SpectraViewer

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.