Cell functional assays: dyes and reagents

Simultaneously analyze multiple proteins, gene expression, and cell functions such as oxidation, viability, cell cycle, apoptosis, and proliferation from an individual cell with fluorescent flow cytometry assays, reagents, and antibodies. This technology makes it possible to obtain a statistically relevant amount of data by combining information from individual cells to gain insight into a heterogeneous sample. We offer flow cytometry assays and reagents that span a broad range of research applications and areas including cell health and function assays as well as RNA detection assays.

Trying to incorporate a reagent into your panel?Ask our technical specialists which you should purchase and how to incorporate into your experiment

Need to add a flow cytometer to your lab?Learn more about the Invitrogen Attune NxT flow cytometer here
 

Figure 1. Cell function workflow with flow cytometry reagents. Use the chart to determine which assays can be incorporated into a panel. Fluorophore reagents can be used to label cell functionalities such as viability and mitochondrial oxidation. These reagents and assays can be incorporated into a flow cytometry panel just like a flow cytometry antibody.


Flow cytometry assays and reagents selections

Product image showing 3 tubes and a packet

Cell viability assays

Distinguishing dead cells and removing artifacts in your assay is a critical step to help ensure accurate results.

Diagram of RNA attached to bDNA and fluorescent probes

RNA assays

Simultaneous detection of RNA and proteins by flow cytometry using the PrimeFlow RNA Assay. Combine the PrimeFlow assay with immunolabeling of both cell-surface and intracellular proteins.

Product image of 10 CellTrace tubes and a packet

Cell proliferation assays

Measuring cell proliferation is a fundamental method for assessing cell health and cell division.

Product image of 5 FxCycle tubes and a box

Cell cycle assays

We offer a series of fluorescent reagents designed for accurate cell cycle analysis in either live- or fixed-cell populations.

Product image of 5 CellRox tubes and a packet

Metabolism and oxidative stress assays

Measure generalized oxidative stress in cells with fluorogenic probes, using conventional fluorescence microscopy, high-content screening, microplate fluorometry, or flow cytometry.

Product image of 1 bottle, 2 LIVE/DEAD baclight tubes and a packet

Microbiology assays

Assess key metabolic processes and monitor bacteria/yeast growth, proliferation, and vitality.

Product image of 1 bottle, 1 CellEvent Senescence tubes and a packet

Senescence

Fluorescent detection of β-galactosidase hydrolysis by flow cytometry.

Product image of 3 CellEvent Caspace3/7 tubes and a packet

Apoptosis assays

Cell death cascades are complex and dynamic, underscoring the importance of a multi-parametric approach to apoptosis detection.

Product image of 2 ReadFlow bottles and a box

Ready-to-use flow cytometry reagents

Invitrogen Ready Flow reagents are room temperature-stable, ready-to-use solutions designed to allow you to stain your cells for analysis by flow cytometry.

Product image of 2 packets of 2 immunophenotyping kits for immune cells

Immunophenotyping kits

The Invitrogen eBioscience essential phenotyping kits are pre-assembled multicolor panels designed to detect immune cells by flow cytometry.


Flow cytometry beads and buffers


Immunology at Work

Learn immune cell type markers and protocols. The Invitrogen Immunology at Work Resource Center is a learning center with technical content designed for new and experienced life scientists alike exploring the field of immunology.

Get started on your experiments

5 Steps to Intracellular Flow Cytometry

Discover products and resources that are available to help optimize your intracellular flow cytometry experiments.

Best practices for performing intracellular flow cytometry

Resources

Flow Cytometry Learning Center—Access flow cytometry educational resources for better experiment planning and execution.

Flow Cytometry Panel Builder—Design your flow cytometry panel with this online tool for a simplified, customizable experience to fit your needs.

5 Steps Resources

Support

Flow Cytometry Support Center—Find technical support recommendations for your flow cytometry workflows, including tips for experimental setup and in-depth troubleshooting help.

Flow Cytometry Panel Design Support—Work with one of our technical sales specialists to discuss your experimental needs and guide you through the process.

Flow Cytometry Protocols

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Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.