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Cell Viability Assays for Flow Cytometry |
Cell viability assays for flow cytometry are used for accurately distinguishing live and dead cell populations, helping ensure the precision of results and analysis. Removing dead and dying cells from your flow cytometry data is critical, as these cells can produce false positive results due to their compromised membranes which allow nonspecific binding and internalization of many reagents. Our flow cytometry viability stains are designed for both fixable and non-fixable cell samples and are available in a wide range of colors, helping ensure compatibility with most instrument configurations. These stains are an excellent choice for various research applications, including immunophenotyping, apoptosis studies, and cell cycle analysis.
Benefits of cell viability assays:
- Enhanced accuracy and reliability of your flow cytometry data
- Broad spectrum of color options for multi-parametric analysis
- Compatibility with a wide range of flow cytometry instruments
- Easy integration into existing workflows
Choose our high-quality viability stains to help improve the accuracy and reliability of your flow cytometry experiments. Explore our product range and find the right solution for your research needs in the selection guide below.
Cell viability assays for flow cytometry selection guide
| Fixable viability dyes | Cell viability assays | LIVE/DEAD cell viability assays | Bacterial viability and vitality assays | Yeast viability and vitality assays | ||
|---|---|---|---|---|---|---|
| Fixability | Fixable | Non-fixable | Non-fixable | Non-fixable Fixable | Non-fixable | |
| Permeability | Cell-impermeant | Cell-impermeant | Cell-impermeant Cell-permeant | Cell-impermeant Cell-permeant | Cell-impermeant Cell-permeant | |
| Kit functionality | Reacts with cellular amine proteins using amine-reactive dyes | Based on membrane integrity and uses nucleic acid binding dyes | Reacts with cellular enzymes | Based on membrane integrity Reacts with cellular enzymes Indicator of metabolic activity | Based on membrane integrity Indicator of reductase activity Evaluates respiratory activity | Based on membrane integrity Reacts with cellular enzymes |
| Cell stained | Dead cells | Dead cells | Live cells | Live cells Dead cells | Live cells Dead cells | Live cells Dead cells |
| Laser | UV – Near IR | UV, violet, blue, yellow, red | UV, violet, blue, yellow | Violet, blue, yellow | Violet, blue, yellow | Blue, yellow |
Explore: Additional Cell Viability Assays
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Cy™ is a trademark or registered trademark of GE Healthcare.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.
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