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Fixable Viability Dyes for Flow Cytometry |
The ability to stain live cells with a viability dye and preserve that staining pattern after fixation is critical for intracellular immunophenotyping. Exclusion of the dead cells from the data allows cleaner separation and identification of cell populations. The Invitrogen LIVE/DEAD Fixable Viability Dyes are fixable viability dyes that help ensure accurate assessment of cell viability in samples after fixation and/or permeabilization.
These flow cytometry–based kits offer you tools that are:
LIVE/DEAD Fixable Dead Cell Stain kits are fixable viability dyes that distinguish live cells from dead cells based on cell membrane integrity and access to available amines. Cells can then be fixed for intracellular antigen detection without loss of original cell staining pattern. Allows easy exclusion of dead cells from flow cytometry readout. Dead cells are highly fluorescent and easy to distinguish from live cells, which are stained with minimal fluorescence.
All products come in three quantity options: 80, 200, 400 assays.
Product | Laser (nm) | Ex/Em (nm) | Dye compatibility |
---|---|---|---|
LIVE/DEAD Fixable Blue Dead Cell Stain Kit | UV | 350/450 | Cannot be used in combination with Invitrogen product line such as Indo-1 (blue), DAPI, or Hoechst 33342 |
LIVE/DEAD Fixable Violet Dead Cell Stain Kit | 405 | 416/451 | Cannot be used in combination with Invitrogen product line such as Pacific Blue dye, CellTrace Violet stain, FxCycle Violet stain, BV421 or eFluor 450 |
LIVE/DEAD Fixable Lime (506) Viability Kit | 405 | 405/506 | Cannot be used in combination with Invitrogen product line such as eFluor 506 and Pacific orange |
LIVE/DEAD Fixable Aqua Dead Cell Stain Kit | 405 | 367/526 | Cannot be used in combination with Invitrogen product line such as Pacific Green dye, F2N12S apoptosis assay, AmCyan, or BV510 |
LIVE/DEAD Fixable Yellow Dead Cell Stain Kit | 405 | 400/575 | Cannot be used in combination with Invitrogen product line such as Pacific Orange dye, Qdot 605, F2N12S apoptosis assay, or BV605 |
LIVE/DEAD Fixable Green Dead Cell Stain Kit | 488 | 495/520 | Cannot be used with NB510, NB530, NB555 |
LIVE/DEAD Fixable Olive (557) Viability Kit | 488 | 480/557 | Can be used with NB510, FITC, AF488. Cannot be used with NB555, NB585 |
LIVE/DEAD Fixable Orange (602) Viability Kit | 561 | 580/602 | Can be used with NB610. Cannot be used with NY590, NY610 |
LIVE/DEAD Fixable Red Dead Cell Stain Kit | 488/561 | 595/615 | Cannot be used with NY610, NY590 |
LIVE/DEAD Fixable Far Red Dead Cell Stain Kit | 633/635 | 650/665 | Cannot be used with NR660 |
LIVE/DEAD Fixable Scarlet (723) Viability Kit | 633/635 | 702/723 | Cannot be used in combination with Invitrogen product line such as Alexa Fluor 700, APC-Cyanine 5.5, NovaFluor Red 700, and DRAQ5 |
LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit | 633/635 | 750/775 | Cannot be used in combination with Invitrogen product line such as APC-Alexa Fluor 750, APC-Cy 7 or Vybrant DyeCycle Ruby stain |
LIVE/DEAD Fixable Near-IR (780) Viability Kit | 633/780 | 633/785 | Cannot be used in combination with Invitrogen product line such as APC-Alexa Fluor 750, APC-eFluor 780 |
LIVE/DEAD Fixable Near-IR (876) Viability Kit | 808 | 840/876 | NA |
Not sure which dye will suit your needs? Try the LIVE/DEAD Fixable Dead Cell Stain Sampler Kit Need compensation bead to use with LIVE/Dead stain kits? Use ArC Amine Reactive Compensation Bead Kit |
Explore : Additional Cell Viability Assays
Explore: Microplate Assays for Cell Viability
LIVE/DEAD Fixable Viability Stain Kits are based on the reaction of a fluorescent reactive dye with cellular proteins (amines). These dyes cannot penetrate live cell membranes, so only cell surface proteins are available to react with the dye, resulting in dim staining (Figure 1, LIVE). The reactive dye can permeate the damaged membranes of dead cells and stain both the interior and exterior amines, resulting in more intense staining (Figure 1, DEAD).
The difference in fluorescence intensity between the live and dead cell populations is typically greater than 50-fold, thereby allowing complete, simultaneous discrimination between the two cell populations (Figure 2A). Additionally, the covalent attachment of the dyes to proteins allows preservation of discrimination of the live and dead cell populations following fixation with formaldehyde, commonly used for intracellular immunophenotyping and to inactivate pathogens (Figure 2B). In contrast, non-fixable dyes including propidium iodide (PI) (Figure 3) lose staining pattern after fixation (Figure 3B).
Figure 3. Cells stained with PI do not retain staining pattern after fixation. Propidium iodide (PI) was used to differentially stain a mixture of live (left peak) and heat-treated Jurkat cells (right peak). Cells in (A) were not fixed; cells in (B) were fixed in 3.7% formaldehyde following staining. Samples were analyzed by flow cytometry using 488 nm excitation and ~617 nm emission.
Protocol: LIVE/DEAD Fixable Dead Cell Stains
This webinar gives an overview of methods and reagents to assess cell viability with flow cytometry. Discussion will focus on our most recent efforts to expand the color palette of fixable viability dyes.
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Cy™ is a trademark or registered trademark of GE Healthcare.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.