Invitrogen™

LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit, for 405 nm excitation

Catalog number: L34963
Invitrogen™

LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit, for 405 nm excitation

Catalog number: L34963
Catalog Number
L34963
Unit Size
80 assays
Price (USD)
Price: 132.00
Online price: 122.65
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
200 assays
Price (USD)
Price: 332.00
Online price: 307.65
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
400 assays
Price (USD)
Price: 383.00
Online price: 354.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
L3496380 assays
Price: 132.00
Online price: 122.65
Your price:
-
L34955200 assays
Price: 332.00
Online price: 307.65
Your price:
-
L34964400 assays
Price: 383.00
Online price: 354.65
Your price:
-
Product Overview
Figures
Videos
Recommendations
Recommendations
Documents
FAQ
Citations & References
Additional Information
Recommendations
The LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. This kit has been optimized and validated for use with a violet laser flow cytometer.

• Stable—dyes are freeze dried in separate vials to maintain stability

• Robust—staining pattern is the same before and after fixation

• Low compensation—minimal spectral overlap between other fluorophores

View a selection guide for all fixable viability dyes for flow cytometry.

Stable
Unlike products that are sold in solution, the LIVE/DEAD™ Fixable Violet Stain has been conveniently packaged in 40-test vials to help ensure the stability and performance of the dye over time. Amine reactive dyes in solution will lose their effectiveness over a short period of time, therefore it is recommended to completely use the vial once rehydrated. If this is not possible, aliquot the vials in small volumes and store at -80°C, avoiding freeze-thaw cycles.

Robust
Dead cell discriminator stains can lose sensitivity after treatment with fixatives such as formaldehyde or ethanol-based fixation methods required for intracellular phosphorylation studies. The LIVE/DEAD™ Fixable Violet Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines, and the staining pattern is preserved following formaldehyde fixation.

Low compensation
The LIVE/DEAD™ Fixable Violet Stain was selected based on its fluorescent properties to minimize compensation between other violet dyes and dyes that excite off of the 488 nm blue laser. The violet-fluorescent reactive dye has an excitation maximum of ∼416 nm and is excited well with the 405 nm violet laser, and it has an emission maxima of ∼451 nm, so it can be collected in the first channel on most violet laser flow cytometers.

How it works
In cells with compromised membranes, the dye reacts with free amines both in the cell interior and on the cell surface, yielding intense fluorescent staining. In viable cells, the dye's reactivity is restricted to the cell-surface amines, resulting in less intense fluorescence. The difference in intensity is typically greater than 50-fold between live and dead cells, allowing for easy discrimination.

Colors available
LIVE/DEAD™ Fixable Dead Cell Stains are available in a wide variety of colors to meet your multi-color panel needs.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Permeability
Impermeant
Cell Type
Eukaryotic Cells
Color
Violet
Description
LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit, for 405 nm excitation
Detection Method
Fluorescence
For Use With (Application)
Viability Assay
For Use With (Equipment)
Flow Cytometer
Product Type
Stain
Dye Type
LIVE/DEAD™ Fixable Violet Dead Cell Stain
Emission
451 nm
Excitation Wavelength Range
416 nm
Form
Solid
Format
Tube(s)
Product Line
LIVE/DEAD™
Quantity
80 Assays
Shipping Condition
Room Temperature
Solubility
DMSO (Dimethylsulfoxide)

Contents & Storage

Contains 2 vials of LIVE/DEAD™ fixable dead cell stain and 500 μL DMSO.

Store at -20°C.

Figures

Documents & Downloads

Certificates

    Frequently asked questions (FAQs)

    Citations & References

    Search citations by name, author, journal title or abstract text