Flow Cytometry Protocols

General protocols for flow cytometry

Super Bright Staining Buffer protocol
Cell Preparation for Flow Cytometry Protocols (Invitrogen eBioscience reagents)
Red Blood Cell Lysis Protocols Using eBioscience Lysis Buffers (Invitrogen eBioscience reagents)
Staining Cell Surface Targets for Flow Cytometry (Invitrogen eBioscience reagents)
Staining Intracellular Antigens for Flow Cytometry (Invitrogen eBioscience reagents)
OneComp and UltraComp Compensation Beads Protocols for Flow Cytometry (Invitrogen eBioscience reagents)

Cell viability protocols for flow cytometry

LIVE/DEAD Fixable Dead Cell Stains Protocol
LIVE/DEAD Sperm Viability Kit Flow Cytometry Protocol
SYTOX Dead Cell Stains Protocol
Viability Staining Protocol for Flow Cytometry (Invitrogen eBioscience reagents)

Cell viability and cell vitality protocols for flow cytometry

LIVE/DEAD Violet Viability/Vitality Kit Protocol
Annexin V Staining Protocol for Flow Cytometry (Invitrogen eBioscience reagents)
Pharmacological Induction of Apoptosis with Camptothecin (Invitrogen eBioscience reagents)

Cell proliferation protocols for flow cytometry

CellTrace™ CFSE Cell Proliferation Kit Protocol
CellTrace™ Far Red Cell Proliferation Kit Protocol
CellTrace™ Violet Cell Proliferation Kit Protocol
BrdU and EdU Double Staining Protocol
Click-iT EdU Protocol for Flow Cytometry
Click-iT Plus EdU Flow Cytometry Assay Kits
BrdU Staining Protocol for Flow Cytometry (Invitrogen eBioscience reagents)
Staining Cells with eFluor Proliferation Dyes for Flow Cytometry (Invitrogen eBioscience reagents)

Cell cycle assay protocols for flow cytometry

Search the full list of cell analysis protocols

Resources

Flow Cytometry Learning Center—Access flow cytometry educational resources for better experiment planning and execution.

Flow Cytometry Panel Builder—Design your flow cytometry panel with this online tool for a simplified, customizable experience to fit your needs.

5 Steps Resources

See all 5 Steps Workflows

Support

Flow Cytometry Support Center—Find technical support recommendations for your flow cytometry workflows, including tips for experimental setup and in-depth troubleshooting help.

Flow Cytometry Panel Design Support—Work with one of our technical sales specialists to discuss your experimental needs and guide you through the process.

Flow Cytometry Protocols

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.