The SYTOX Dead Cell Stains are nucleic acid stains for assessing cell viability with flow cytometry. Dead cells have bright fluorescence and live cells have dim fluorescence. SYTOX Dead Cell Stains are available in five different colors.
|1. Thaw vial of dye
|2. Add 1 mL cells to a flow cytometer tube
|3. Add 1 µL dye to cells and mix well
|4. Incubate for 15 minutes
|5. Run cells on a flow cytometer
|Flow cytometer channel
|Alexa Fluor 647
|The SYTOX Dead Cell Stain Sampler Kit (Cat. No. S34862) contains trial-sized samples of 5 different stains (50 tests per stain) to help researchers identify the best stain for their multicolor experiments.
A mixture of heat-killed and live Jurkat cells were labeled with the SYTOX Dead Cell stains. All stains were labeled according to the listed protocol. Samples were analyzed on a flow cytometer equipped with a 488 nm laser, a 405 nm laser, or a 633 nm laser, and fluorescence emissions were collected using the appropriate filters.