Cell proliferation can be measured with the thymidine analog BrdU (5-bromo-2’-deoxyuridine) following its incorporation into newly synthesized DNA and its subsequent detection with an anti-BrdU antibody.
This protocol can be used for:
Detecting DNA synthesis using a fluorescence microscope
This protocol should not be used for:
You will need the following for this protocol:
Proliferating adherent cells in an appropriate vessel for imaging