Membrane integrity-based viability assay
This kit includes Image-iT DEAD Green viability stain for identification of dead cells. After staining your cells, the signal from the stain will be retained if the sample is fixed and permeabilized.
This protocol can be used for:
- Identifying dead cells using a fluorescence microscope
This protocol should not be used for:
- Flow cytometry
You will need the following for this protocol:
- Cells growing in culture
- Image-iT DEAD Green Viability Stain (Cat. No. I10291)
- Phosphate-buffered saline (PBS) (Cat. No. 14190-144)
- Anhydrous DMSO (Cat. No. D12345)
- Fluorescence microscope with FITC filters
Protocol
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Spectral information and storage
Image-iT DEAD Green | |
---|---|
Excitation/Emission | 488/515 nm |
Standard filter set | FITC or GFP |
EVOS Light Cube | GFP |
Storage conditions | Room temperature |
Protocol tips
- This kit is compatible with antibody labeling protocols
- Dye stock solutions should be stored frozen
Valinomycin-treated HeLa cells labeled with the Image-iT DEAD Green Viability Stain.