The Invitrogen CellTrace CFSE kit is used to monitor distinct generations of proliferating cells by dye dilution. Live cells are covalently labeled with a very bright, stable dye. Every generation of cells appears as a different peak on a flow cytometry histogram.
|Excitation/Emission (in nm)||492/517|
|Standard filter set||Invitrogen Alexa Fluor 488|
Human T lymphocytes stained with the CellTrace CFSE Cell Proliferation Kit and stimulated in culture for 5 days. The discrete peaks represent successive generations of live cells. The unstimulated parent generation is indicated in blue. Analysis was completed using an Attune Acoustic Focusing Cytometer with 488 nm excitation and a 530/30 nm bandpass emission filter.
For Research Use Only. Not for use in diagnostic procedures.