Two-color nuclear staining assay for cell viability
This kit can be used to quickly and easily determine the viability of cells. NucBlue® Live reagent stains the nuclei of all cells, while propidium iodide stains only the nuclei of dead cells.
This protocol can be used for:
- Identifying live and dead cells using a fluorescence microscope
This protocol should not be used for:
- Flow cytometry
|1. Culture cells in appropriate medium and vessel for microscopy
|2. Add 2 drops each NucBlue® Live and propidium iodide per milliliter of medium to label cells|
|3. Incubate 5–30 minutes
|4. Image cells
Spectral information and storage
|NucBlue® Live||Propidium iodide|
|Excitation/Emission||360/460 nm||535/617 nm|
|Standard filter set||DAPI||TRITC or RFP|
|EVOS® Light Cube||DAPI||TRITC or RFP|
|Storage conditions||Room temperature||Room temperature|
- In some cases, more or fewer drops may be needed to achieve optimal staining intensity
- Image quality may be improved by replacing media with Live Cell Imaging Solution
Jurkat cells stained with NucBlue® Live reagent and propidium iodide.
For Research Use Only. Not for use in diagnostic procedures.