Two-color nuclear staining assay for cell viability
This kit can be used to quickly and easily determine the viability of cells. NucBlue Live reagent stains the nuclei of all cells, while propidium iodide stains only the nuclei of dead cells.
This protocol can be used for:
- Identifying live and dead cells using a fluorescence microscope
This protocol should not be used for:
- Flow cytometry
You will need the following for this protocol:
- Cells growing in culture
- ReadyProbes Cell Viability Imaging Kit, Blue/Red (Cat. No. R37610)
- Fluorescence microscope with DAPI and RFP or TRITC filters
Protocol
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Spectral information and storage
NucBlue Live | Propidium iodide | |
---|---|---|
Excitation/Emission | 360/460 nm | 535/617 nm |
Standard filter set | DAPI | TRITC or RFP |
EVOS Light Cube | DAPI | TRITC or RFP |
Storage conditions | Room temperature | Room temperature |
Protocol tips
- In some cases, more or fewer drops may be needed to achieve optimal staining intensity
- Image quality may be improved by replacing media with Live Cell Imaging Solution
Jurkat cells stained with NucBlue Live reagent and propidium iodide.