Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
A popular nuclear and chromosome counterstain, DAPI (4′,6-diamidino-2-phenylindole) emits blue fluorescence upon binding to AT regions of DNA. This protocol produces sufficient stain for one 96-well plate at a staining volume of 100 μL/well.
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First, fix and permeabilize cultured cells with a protocol appropriate for your sample.
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DAPI | |
---|---|
Excitation/Emission (nm) | 358/461 |
Standard filter set | DAPI |
EVOS Light Cube | DAPI |
Storage conditions | 2–6°C or ≤–20°C |
Cells stained with DAPI and imaged with the Thermo Scientific CellInsight High-Content System.