ActinRed 555 ReadyProbes Reagent is a selective, high-affinity F-actin probe conjugated to the red-orange fluorescent dye tetramethylrhodamine (TRITC). Like other ReadyProbes reagents, ActinRed 555 reagent is formulated as a room temperature–stable solution and comes in a convenient dropper bottle.
First, fix and permeabilize cultured cells with a protocol appropriate for your sample. Counterstain as desired. DAPI may be added directly to fixative.
|1. Wash the cells 1–3 times in PBS as needed.|
|2. Add two drops of ActinRed 555 ReadyProbes Reagent per milliliter of medium.|
|3. Incubate for 30 minutes, protected from light.|
|4. Remove the stain solution.|
|5. Wash the cells 2–3 times in PBS.|
|6. Image the cells.|
|Standard filter set||TRITC|
|EVOS Light Cube||TRITC|
|Storage conditions||Room temperature|
For Research Use Only. Not for use in diagnostic procedures.