Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
ActinRed 555 ReadyProbes Reagent is a selective, high-affinity F-actin probe conjugated to the red-orange fluorescent dye tetramethylrhodamine (TRITC). Like other ReadyProbes reagents, ActinRed 555 reagent is formulated as a room temperature–stable solution and comes in a convenient dropper bottle.
First, fix and permeabilize cultured cells with a protocol appropriate for your sample. Counterstain as desired. DAPI may be added directly to fixative.
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ActinRed 555 | |
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Excitation/Emission (nm) | 540/565 |
Standard filter set | TRITC |
EVOS Light Cube | TRITC |
Storage conditions | Room temperature |
Multicolor staining of BPAE cells. BPAE cells were fixed and permeabilized using the Image-iT Fixation/Permeabilization Kit. Actin was stained using ActinRed 555 ReadyProbes Reagent, and nuclei were stained with NucBlue Fixed Cell ReadyProbes Reagent.
For Research Use Only. Not for use in diagnostic procedures.