ReadyProbes Cell Viability Imaging Kit, Blue-Green

Two-color nuclear staining assay for cell viability

This kit can be used to quickly and easily determine the viability of cells. NucBlue Live reagent stains the nuclei of all cells, while NucGreen Dead reagent stains only the nuclei of dead cells.

This protocol can be used for:

  • Identifying live and dead cells using a fluorescence microscope

This protocol should not be used for:

  • Flow cytometry

You will need the following for this protocol:


1. Culture cells in appropriate medium and vessel for microscopy
2. Add 2 drops each NucBlue Live and NucGreen Dead reagents per milliliter of medium to label cells
3. Incubate 5–30 minutes
4. Image cells

Spectral information and storage

 NucBlue LiveNucGreen Dead
Excitation/Emission360/460 nm504/523 nm
Standard filter setDAPIFITC/GFP
Storage conditionsRoom temperatureRoom temperature


Protocol tips