Two-color nuclear staining assay for cell viability

This kit can be used to quickly and easily determine the viability of cells. NucBlue® Live reagent stains the nuclei of all cells, while NucGreen® Dead reagent stains only the nuclei of dead cells.

This protocol can be used for:

  • Identifying live and dead cells using a fluorescence microscope

This protocol should not be used for:

  • Flow cytometry

You will need the following for this protocol:

Protocol

1. Culture cells in appropriate medium and vessel for microscopy
2. Add 2 drops each NucBlue® Live and NucGreen® Dead reagents per milliliter of medium to label cells
3. Incubate 5–30 minutes
4. Image cells

Spectral information and storage

  NucBlue® Live NucGreen® Dead
Excitation/Emission 360/460 nm 504/523 nm
Standard filter set DAPI FITC/GFP
EVOS® Light Cube DAPI GFP
Storage conditions Room temperature Room temperature

 

Protocol tips

Image of cells stained with NucBlue Live and NucGreen Dead reagents
Jurkat cells stained with ReadyProbes® (Blue/Green) Cell Viability Imaging Kit.