NucRed® Dead 647 ReadyProbes® Reagent Protocol
Membrane integrity—based viability assay
NucRed® Dead 647 reagent is a DNA stain that labels dead or dying cells, but does not enter living cells.
This protocol can be used for:
- Identifying dead cells using a fluorescence microscope
This protocol should not be used for:
- Flow cytometry
You will need the following for this protocol:
- Cells growing in culture
- NucRed® Dead 647 reagent (Cat. No. R37113)
- Fluorescence microscope with far red or Cy®5 filters
|1. Culture cells in appropriate medium and vessel for microscopy
|2. Add 2 drops NucRed® Dead 647 reagent per milliliter of medium to label cells
|3. Incubate 5–15 minutes
|4. Image cells
Spectral information and storage
|NucRed® Dead 647 ReadyProbes® Reagent|
|Standard filter set||Cy®5|
|EVOS® Light Cube||Cy®5|
|Storage conditions||Room temperature|
- In some cases, more or fewer drops may be needed to achieve optimal staining intensity
- Image quality may be improved by replacing medium with Live Cell Imaging Solution (A14291DJ)
HeLa cells stained with NucRed® Dead 647 ReadyProbes® Reagent.
For Research Use Only. Not for use in diagnostic procedures.