Membrane integrity—based viability assay

NucRed® Dead 647 reagent is a DNA stain that labels dead or dying cells, but does not enter living cells.

This protocol can be used for:

  • Identifying dead cells using a fluorescence microscope

This protocol should not be used for:

  • Flow cytometry

You will need the following for this protocol:


1. Culture cells in appropriate medium and vessel for microscopy
2. Add 2 drops NucRed® Dead 647 reagent per milliliter of medium to label cells
3. Incubate 5–15 minutes
4. Image cells

Spectral information and storage

  NucRed® Dead 647 ReadyProbes® Reagent
Excitation/Emission 642/661 nm
Standard filter set Cy®5
EVOS® Light Cube Cy®5
Storage conditions Room temperature


Protocol tips

Image of cells stained with NucRed Dead 647 ReadyProbes Reagent

HeLa cells stained with NucRed® Dead 647 ReadyProbes® Reagent.