SYTOX Green Nucleic Acid Stain is a bright, high-affinity nucleic acid stain that easily penetrates cells with compromised plasma membranes but does not cross the membranes of live cells, making it a useful indicator of dead cells within a population.
First, fix and permeabilize cultured cells with a protocol appropriate for your sample.
1. Wash the cells 1–3 times in a phosphate-free buffer as needed.
|2. Prepare the SYTOX Green staining solution by diluting the stock solution 1:30,000 (167 nM) in a phosphate-free buffer.|
|3. Add sufficient staining solution to cover the cells.|
|4. Incubate for 15–30 minutes, protected from light.|
|5. Remove the staining solution.|
|6. Wash the cells 2–3 times in a phosphate-free buffer.|
|7. Image the cells.|
|Standard filter set||GFP|
|EVOS Light Cube||GFP|
For Research Use Only. Not for use in diagnostic procedures.