NucBlue® Live ReadyProbes® Reagent Protocol
Nuclear stain for live cells
NucBlue® Live reagent (Hoechst® 33342 dye) stains the nuclei of all cells. NucBlue® Live reagent is frequently used to distinguish condensed nuclei in apoptotic cells.
This protocol can be used for:
- Nuclear staining to identify nuclear condensation in apoptotic cells using fluorescence microscopy
This protocol should not be used for:
- Flow cytometry
You will need the following for this protocol:
- Cells growing in culture
- NucBlue® Live ReadyProbes® Reagent (Cat. No. R37605)
- Fluorescence microscope equipped with a DAPI filter
|1. Culture cells in an appropriate medium and vessel for fluorescence microscopy
|2. Add two drops of NucBlue® Live ReadyProbes® Reagent per milliliter of medium
|3. Incubate for 20 minutes, protected from light.
|4. Image the cells.
|Standard filter set||DAPI|
|EVOS® Light Cube||DAPI|
|Storage conditions||Room temperature|
- In some cases, more or fewer drops may be needed to achieve optimal staining intensity.
- Image quality may be improved by replacing the culture medium with Live Cell Imaging Solution (Cat. No. A14291DJ).
U2OS live-cell staining. Live U2OS cells were imaged 15 minutes following staining with NucBlue® Live ReadyProbes® Reagent.