NucBlue Fixed Cell ReadyProbes Reagent is a bright blue cell-impermeant nuclear stain for fixed cells. It is useful to distinguish the condensed nuclei of apoptotic cells. It is also suitable for staining nuclei in fixed-cell preparations and tissue sections.
First, fix and permeabilize cultured cells with a protocol appropriate for your sample.
|1. Wash the cells 1–3 times in PBS as needed.
|2. Add two drops of NucBlue Fixed Cell ReadyProbes Reagent per milliliter of medium.
|3. Incubate for 5 minutes at 20–25°C.
|4. Image the cells.
|Standard filter set
|EVOS Light Cube