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Gibco™

Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation

Catalog number: 11161D
Gibco™

Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation

Catalog number: 11161D
Catalog Number
11161D
Unit Size
0.4 mL
Price (USD)
172.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
2 mL
Price (USD)
764.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
5 x 2 mL
Price (USD)
2,285.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
11161D0.4 mL
172.00
Your Price:
***
11131D2 mL
764.00
Your Price:
***
11132D5 x 2 mL
2,285.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Dynabeads® Human T-Activator CD3/CD28 are for the activation and expansion of human T cells. Advantages of Dynabeads® Human T-Activator CD3/CD28:

• Activation of T cells without the need for feeder cells
• Activated cells that retain in vivo-like function
• Maximum reproducibility without contamination by soluble antibodies or mitogens
• From 100- to 1,000-fold T cell expansion

About Dynabeads® Human T-Activator CD3/CD28
Dynabeads® Human T-Activator CD3/CD28 offer a simple method for activation and expansion of T cells that does not require feeder cells (antigen-presenting cells) or antigen. The uniform 4.5 µm diameter, inert, superparamagnetic beads are similar in size to antigen-presenting cells, and are covalently coupled to anti-CD3 and anti-CD28 antibodies. These two antibodies provide primary and co-stimulatory signals, optimized for efficient T cell activation and expansion. Expansion of the T cell population can be stimulated using recombinant IL-2, and after activation or expansion, the magnetic beads can be easily removed using a DynaMag™ magnet. For expansion of antigen-specific T cells from T cell clones or T cell lines, we recommend using Dynabeads® Human T-Activator CD3/CD28/CD137.

Starting samples
Start with mononuclear cells (MNCs)/peripheral blood mononuclear cells (PBMCs) from either whole blood or buffy coat, or T cell subsets including CD3+ T cells, CD4+ or CD8+ T cells, or regulatory T cells.

Downstream applications
The activated T cells can be analyzed shortly after activation (for transfection/transduction or to study TCR signaling, proteomics, gene expression, etc.). T cells can be left in culture to differentiate into T helper cell subsets or expansion of polyclonal/antigen-specific T cells.

Learn more about Dynabeads® products
• Find Dynabeads® products for a whole range of applications.
• Find magnets for Dynabeads® separations.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type
T Cells (Whole Population)
Clonality
Monoclonal
Final Product Type
Activated, expanded T cells
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Host Species
Mouse
Isolation Technology
Dynabeads Untouched or Dynabeads FlowComp
Ligand Type
Antibody
No. of Tests
200
Product Line
DYNAL™, Dynabeads™
Purity or Quality Grade
Research Grade
Reactivity
Human
Shipping Condition
Room Temperature
Surface Functionality
Epoxy
Target Species
Human
Format
Bead
Quantity
0.4 mL

Contents & Storage

The product contains 0.4 ml Dynabeads Human T-Activator CD3/CD28 in phosphate buffered saline (PBS), pH 7.4, containing 0.1% human serum albumin (HSA). Store at 2–8°C

4 × 107 beads/ml suspension.

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Frequently asked questions (FAQs)

Citations & References