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Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation

Catalog number:  11161D

Related applications:

Cell Isolation & Expansion

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Dynabeads® Human T-Activator CD3/CD28 are for the activation and expansion of human T cells. Advantages of Dynabeads® Human T-Activator CD3/CD28:

• Activation of T cells without the need for feeder cells
• Activated cells that retain in vivo-like function
• Maximum reproducibility without contamination by soluble antibodies or mitogens
• From 100- to 1,000-fold T cell expansion

About Dynabeads® Human T-Activator CD3/CD28
Dynabeads® Human T-Activator CD3/CD28 offer a simple method for activation and expansion of T cells that does not require feeder cells (antigen-presenting cells) or antigen. The uniform 4.5 µm diameter, inert, superparamagnetic beads are similar in size to antigen-presenting cells, and are covalently coupled to anti-CD3 and anti-CD28 antibodies. These two antibodies provide primary and co-stimulatory signals, optimized for efficient T cell activation and expansion. Expansion of the T cell population can be stimulated using recombinant IL-2, and after activation or expansion, the magnetic beads can be easily removed using a DynaMag™ magnet. For expansion of antigen-specific T cells from T cell clones or T cell lines, we recommend using Dynabeads® Human T-Activator CD3/CD28/CD137.

Starting samples
Start with mononuclear cells (MNCs)/peripheral blood mononuclear cells (PBMCs) from either whole blood or buffy coat, or T cell subsets including CD3+ T cells, CD4+ or CD8+ T cells, or regulatory T cells.

Downstream applications
The activated T cells can be analyzed shortly after activation (for transfection/transduction or to study TCR signaling, proteomics, gene expression, etc.). T cells can be left in culture to differentiate into T helper cell subsets or expansion of polyclonal/antigen-specific T cells.

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For Research Use Only. Not for use in diagnostic procedures.


Cell Type: T Cells (Whole Population)
Clonality: Monoclonal
Final Product Type: Activated, expanded T cells
Form: Bead
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Host Species: Mouse
Isolation Technology: Dynabeads Untouched or Dynabeads FlowComp
Ligand: Antibody
No. of Reactions: 200 Tests
Product Line: DYNAL®, Dynabeads®
Purity or Quality Grade: Research Grade
Reactivity: Human
Shipping Condition: Room Temperature
Surface Functionality: Epoxy
Target Species: Human
Quantity: 0.4mL

Contents & storage

The product contains 0.4 ml Dynabeads Human T-Activator CD3/CD28 in phosphate buffered saline (PBS), pH 7.4, containing 0.1% human serum albumin (HSA). Store at 2–8°C

4 × 107 beads/ml suspension.