Informational:See a minimum of 6 generations using the new CellTrace™ Far Red Proliferation Kit for Flow Cytometry.
Catalog Number
Unit Size
180 reactions
Price (USD)
Price: 237.00
Your Price:
Catalog Number
Unit Size
20 reactions
Price (USD)
Price: 122.00
Your Price:
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C34554180 reactions
Price: 237.00
Your Price:
C3457020 reactions
Price: 122.00
Your Price:

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Product Overview
Citations & References
Additional Information

CellTrace™ CFSE Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry.

• Superior performance—bright, single-peak staining enables visualization of multiple generations
• Long-term signal stability—well-retained in cells for several days post stain
• Non-toxic—staining does not adversely effect cell health
• Simple, robust staining protocol

View a selection guide for all CellTrace™ Cell Proliferation Kits for flow cytometry.

Superior fluorescent staining
Successful proliferation analysis by dye dilution (see figure below) requires an extremely bright dye to distinguish fluorescently labeled cells from auto-fluorescence after several cell divisions. The intense fluorescent staining provided by CellTrace™ CFSE dye enables the visualization of eight or more generations of proliferating cells before the signal is overwhelmed by intrinsic cellular auto-fluorescence. Consistent, homogeneous staining results in very little fluorescence variation between cells in a population, so distinct generations can be seen without any requirement for complex modeling software.

Long-term signal retention
Unlike stains that label the lipid membrane of cells, CellTrace™ CFSE dye easily crosses the plasma membrane and covalently binds inside cells where the stable, well-retained fluorescent dye is designed to provide a consistent signal, even after several days in a cell culture environment.

CellTrace™ CFSE dye binds covalently to all free amines on the surface and inside of cells and shows little cytotoxicity, with minimal observed effect on the proliferative ability or biology of cells. Researchers have used CFSE SE labeling to show that transplantable hematopoietic cells proliferate in vitro in response to stimulation by a growth factor cocktail.

Simple, robust staining protocol
The CellTrace™ CFSE Cell Proliferation Kit contains convenient single-use vials of dry dye to permit small-scale experiments without preparing excess quantities of dye. A stock solution is prepared by dissolving the contents of a vial in anhydrous DMSO prior to use. To stain 1 mL of cells in protein-free medium, 1 µL of this stock solution is typically used. Cells should be stained for 20 minutes at room temperature with gentle agitation. A brief wash with complete medium will then quench any dye remaining in solution.

Consult user Manual for solubility instructions.

For Research Use Only. Not for use in diagnostic procedures.


CellTrace™ Cell Proliferation Kit, 180 rxns, CFSE, Lyophilized, Fluorescent Detection Method, 492/517nm Excitation/Emission Wavelength, CellTrace™ CFSE Label/Dye, –5 to –30°C Storage, Use with Flow Cytometer
Detection Method
For Use With (Application)
Proliferation Assay, Flow Cytometry
For Use With (Equipment)
Flow Cytometer
Product Type
Cell Proliferation Kit
Dye Type
Other Label(s) or Dye(s)
488 nm
Product Line
1 Kit(s)
Reagent Type
CFSE & Related Compounds
Shipping Condition
Room Temperature
DMSO (Dimethylsulfoxide)
Sufficient For
180 Reactions

Contents & Storage

Contains 10 vials of CellTrace™ CFSE (lyophilized powder) and 1 vial of DMSO (500 μL). Store in freezer (-5°C to -30°C) and protect from light.