Customer concerns surrounding the expiration dates of Penicillin-Streptomycin-Glutamine (100X) have prompted the development of a new formulation with a 12-month shelf life.

Penicillin-Streptomycin-Glutamine (100X)

Catalog number: 10378016

Gibco™  Related applications: Mammalian Cell Culture

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The antibiotics penicillin and streptomycin are used to prevent bacterial contamination in cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. Penicillin was originally purified from the fungi Penicillium and acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus . It acts by binding to the 30S subunit of the bacterial ribosome, leading to the inhibition of protein synthesis and death in susceptible bacteria. This solution contains 10,000 units of penicillin, 10,000 µg of streptomycin, and 29.2 mg/ml of L-glutamine in a 10 mM citrate buffer (for pH stability).

We offer a wide range of antibiotics and antimycotics in both powder and liquid formats. See the complete list, or find products for:
Contamination control
Eukaryotic and bacterial selection

See recommended working concentrations for selection antibiotics.

Learn more about the use of antibiotics and antimycotics in cell culture and review guidelines for decontaminating cultures.
For Research Use Only. Not for use in diagnostic procedures.


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Validated Application: Prevention of Cell Culture Contamination
Agent: L-Glutamine, Penicillin, Streptomycin
Form: Liquid
Reagent Type: Antibiotic, Supplement
Concentrated: 100 X
Product Size: 100 mL
Concentration: 50 mg⁄mL
Shipping Condition: Dry Ice

Contents & storage

Storage conditions: -5°C to -20°C
Shipping conditions: Dry ice
Shelf life: 12 months from date of manufacture


Manuals & protocols

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