Countess Automated Cell Counter

Accurate counts in as few as 10 seconds

Thermo Fisher Scientific offers two high-performance automated cell counters designed to meet the needs of any lab. The Invitrogen Countess II and Countess II FL Automated Cell Counters contain advanced autofocusing and counting algorithms to allow you to quickly and accurately count cells, while avoiding user variation associated with counting.

  • Accuracy—autofocus and auto-lighting minimize user-to-user variability
  • Speed—results in as little as 10 seconds
  • Convenience—build-in dilatation calculator and ability to save up to 10 user profiles

Request a demo  Request a customized package quote

Download the Automated Cell Counters brochure

Countess II Automated Cell Counter
Countess II FL Automated Cell Counter

Countess II FL Automated Cell Counter

  • Brightfield plus fluorescence counting
  • Reusable slides option

Compare with Countess II
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Accuracy—autofocus and auto-lighting minimize user-to-user variability

The Countess Automated Cell Counter contains advanced autofocusing and counting algorithms to allow you to quickly and accurately identify and count cells within a population (Figure 1). Countess instruments are compatible with a broad range of cell types and cell lines, and we have verified their use on a number of commonly used cell lines. Upon insertion of the slide the Countess instrument will automatically focus on cells, which helps to minimize user variation associated with manual focusing. Cells between ~7 µm and 60 µm and in suspension at a density of 1 x 104 to 1 x 107 cells/mL are optimal for counting.

graph showing accurate cell counts using the Countess II Automated Cell Counter

Figure 1. Autofocusing and advanced counting algorithms are designed for more precise counting. NIH/3T3, HeLa, and HL-60 cells were counted using a Countess II FL Automated Cell Counter (blue bar) or counted manually under a microscope using a hemocytometer (purple bar). Each bar is an average of 10 counts.

Clumpy cells are not a problem

Clumpy cells are not a problem for the Countess Automated Cell Counter. Whether you currently use automated or manual cell counting methods, obtaining accurate cell counts from samples containing clumpy cells can be difficult. The advanced counting algorithms of the Countess Automated Cell Counter can clearly identify cell boundaries within clumps of cells, resulting in accurate cell counts (Figure 2).

screenshot of Countess II FL counting clumpy cells

Figure 2. Accurate cell counts, even with clumpy samples. The segmentation and counting algorithms of the Countess II FL Automated Cell Counter are able to resolve clumps of cells in this NIH/3T3 cell sample.

Speed—results in as few as 10 seconds

The time it takes to manually count cells using a hemocytometer (up to 5 minutes) compared to counting with the Countess II automated cell counters (10 seconds) is often overlooked as an added cost. An individual counting five slides per day (two samples per slide) can save ~10 hours per month by switching to an automated counter with a reusable slide. The time savings increases to ~15 hours per month when using disposable slides (Figure 3). This additional time can be applied to other activities in the lab, resulting in a significant advantage when switching from manual to automated cell counting.

Bar chart showing time saved by switching to automated cell counting.

Figure 3. Estimated hours saved per month when switching from counting cells with a hemocytometer to using an automated cell counter.

Convenience—built-in dilution calculator and ability to save up to 10 user profiles

Built-in dilution calculator

Easily determine the amount of cell sample and buffer needed for your experiment with the built-in dilution calculator. The cell count results are automatically used in the calculation; simply input the desired concentration and the volume needed.

Countess dilution calculator screen result showing the amount of cell solution needed.

Easily gate cells and view histograms

Cells can be gated based on cell size, brightness, and circularity to fine-tune what is included or excluded for specific applications. View the histogram by selecting the graph icon, and watch the changes in real time as you use the gating features. The average cell size is also displayed on the histogram.

Counting result screen showing average size of cells and sliders to adjust 3 parameters.

Streamline your workflow with custom profiles

Custom profiles can be saved and easily accessed from the capture and results screens. Settings for cell size, brightness, and circularity as well as fluorescence thresholds can be customized and saved for easy retrieval.

Profiles screen showing the option of 9 custom counting profiles.

Printable report with cell counting results

Using the USB port on the Countess II and II FL Automated Cell Counters, you can save your cell count results and images and then transfer them to your own computer. Image files can be saved as TIF, PNG, or JPG files, and the results are saved as a CSV file. A printable PDF report with results, images, and settings used can also be saved.

Countess II FL Report

Compare Countess II and II FL cell counters

Feature Countess II counter Countess II FL counter
Counting mode Brightfield Brightfield channel plus two optional, user-changeable fluorescence channels
Slides Disposable slides Reusable and disposable slides
Counting time As few as 10 seconds
Focus Autofocus with manual focus option
User profiles Customize and save up to 10 user profiles
User interface Intuitive touch screen with ability to use a mouse, if desired

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Countess II Automated Cell Counter ordering information

Cat. No. Name
Instrument (instrument includes one-year standard warranty)
AMQAX1000 Countess II Automated Cell Counter
Instrument with extended warranty* See service plan overview
A27978 Instrument + 3 yr Rapid Exchange (REX) extended warranty
A27977 Instrument + 3 yr AB Repair Center (ABRC) Plus extended warranty
Package
  Countess II Automated Cell Counter Productivity Package
We provide you a custom quote upon the selection from following items:
  • Countess II Automated Cell Counter
  • Invitrogen Trypan Blue Stain (0.4%)
  • Countess Cell Counting Chamber Slides
  • 3 years Rapid Exchange Extended Warranty
Request a quote
Consumables
C10228 Countess Cell Counting Chamber Slides (50)
C10312 Countess Cell Counting Chamber Slides (500)

*Learn more about Rapid Exchange (REX)
*Learn more about AB Repair Center (ABRC) Support

Countess II FL Automated Cell Counter ordering information

Cat. No. Name
Instrument (instrument includes one-year standard warranty)
AMQAF1000 Countess II FL Automated Cell Counter
Instrument with extended warranty* See service plan overview
A27975 Instrument + 1 yr Rapid Exchange (REX) extended warranty
A27976 Instrument + 3 yr Rapid Exchange (REX) extended warranty
A27974 Instrument + 3 yr AB Repair Center (ABRC) Plus extended warranty
Package
A32136 Countess II FL Automated Cell Counter Starter Package
This kit includes:
  • Countess II FL Automated Cell Counter
  • Countess II FL Reusable Slide
  • Countess Cell Counting Chamber Slides (500 slides)
  • EVOS Light Cubes (RFP and GFP)
  Countess II FL Automated Cell Counter Productivity Package
We provide you a custom quote upon the selection from following items:
  • Countess II FL Automated Cell Counter
  • Countess II FL Reusable Slide
  • Countess Cell Counting Chamber Slides
  • Invitrogen Trypan Blue Stain (0.4%)
  • EVOS Light Cube, GFP
  • EVOS Light Cube, RFP
  • EVOS Light Cube, DAPI
  • Live/Dead Viability/Cytotoxicity Kit (Green/Red)
  • 3 years Rapid Exchange Extended Warranty
Request a quote
Consumables
A25750 Countess II FL Reusable Slide
C10228 Countess Cell Counting Chamber Slides (50)
C10312 Countess Cell Counting Chamber Slides (500)

*Learn more about Rapid Exchange (REX)
*Learn more about AB Repair Center (ABRC) Support

Service plan overview

  Rapid Exchange (REX) AB Repair Center (ABRC) Support
Plan is designed for Labs that operate under critical timelines Regulated labs that must maintain asset tagging consistency to comply with regulated protocols
Repair time Replacement shipped out in 1 business day Your instrument repaired and returned in ~3 weeks*
Off-site repair service including shipping, parts, and labor    
High-priority telephone and email access to our remote service center    
* For added convenience upgrade to ABRC Support Plus contract, which includes a loan instrument to be shipped within 1 business day.

Learn more about Countess instrument service plans