EdU—A Superior BrdU Alternative for Cell Proliferation

Replace your cumbersome BrdU assays

The Invitrogen Click-iT EdU cell proliferation assays combine EdU labeling with powerful click chemistry to provide a superior alternative to traditional BrdU staining methods for detecting and quantitating newly synthesized DNA.

  • Fast—Detection in as little as 80 min, without an antibody
  • Mild conditions—No denaturation required
  • Reproducible—Consistent staining from robust protocols

Cell Proliferation Assay Protocols  Cell Cycle & Proliferation Pathways


EdU staining with Click-iT technology, EdU vs BrdU assay

The simplicity of the click detection method makes the EdU assay a faster, friendlier alternative to the BrdU assay.


BrdU assay

In the traditional BrdU assay, cells proliferating in the window of time that the thymidine nucleoside analog BrdU is present, will incorporate BrdU into newly synthesized DNA. These proliferating cells can be identified using an antibody-based BrdU staining protocol that is amenable to staining with other cell surface and/or intracellular targets of interest in flow cytometry. In microscopy experiments, BrdU labeling and detection can be combined with secondary antibodies.
 

Figure 1. BrdU staining with antibody detection. Denaturation is required for detection of BrdU that is incorporated into newly synthesized DNA. Acid, heat, and nuclease treatment are options for denaturation. These reagents not only induce unwanted artifacts due to the harsh conditions but also extend the time to detection. Such severe treatments can result in inconsistent staining and decreased staining signal. The protocol shown is for processing rat tissue sections. Choose from optimized kits for BrdU kits for flow cytometry, and anti-BrdU antibodies.
 

EdU assay

Unlike assays using BrdU staining, Click-iT EdU assays are not antibody-based and therefore do not require DNA denaturation for detection of the incorporated nucleoside. In addition, for increased utility, the Click-iT Plus EdU assay can be multiplexed with R-phycoerythrin (R-PE) and R-PE tandems, fluorescent proteins (GFP and mCherry), and also with BrdU in a BrdU and EdU double staining experiment. The Click-iT EdU technology has not only been developed into kits for flow cytometry and imaging applications (including HCS), but it also has been adapted for the colorimetric detection of EdU in an immunohistochemistry (IHC) assay. When you compare the methods side-by-side (Figures 1 and 2), the benefits of the Click-iT EdU assay and Invitrogen Click-iT Plus EdU assay for cell proliferation are clear.

 

Figure 2. EdU staining with Click-iT and Click-iT Plus detection. EdU that is incorporated into newly synthesized DNA is detected without the need for DNA denaturation. Simple, easy-to-follow, robust protocols allow for reproducible, bright staining. The Click-iT EdU Assay kits work with most standard fluorophore conjugates. Click-iT Plus EdU Assay kits were designed for maximum multiplex flexibility and are compatible with R-PE (and tandems) and fluorescent proteins such as GFP and mCherry. Protocol shown is for processing rat tissue sections. Optimized kits developed for use in flow cytometry, imaging, microplates, high-content screening, and colorimetric immunohistochemistry applications.


Click-iT EdU assays for flow cytometry

Ideal for maximum multiplexability in your flow cytometry experiments. Compatible with R-PE, R-PE tandems, and fluorescent proteins such as GFP and mCherry.
 

 Click-iT Plus EdU Alexa Fluor 350 Flow Cytometry KitClick-iT Plus EdU Pacific Blue Flow Cytometry Kit Click-iT Plus EdU Alexa Fluor 488 Flow Cytometry KitClick-iT Plus EdU Alexa Fluor 594 Flow Cytometry KitClick-iT Plus EdU Alexa Fluor 647 Flow Cytometry Kit
Basis of assay
  • Incorporation of EdU into newly synthesized DNA
  • Detection using a fast, highly specific click reaction of a picolyl azide (Click-iT Plus technology)
  • Labeled with photostable Alexa Fluor dyes or Pacific Blue
MultiplexableMaximum multiplexability
  • Standard organic dyes (FITC, Alexa Fluor dyes, etc.)
  • R-PE, R-PE tandems (such as R-PE-Cy5 or R-PE-Cy7)
  • Fluorescent proteins (such as GFP and mCherry)
  • Common cell staining methods
ReadoutFluorescent signal accumulates in the nuclei of cells where DNA has been synthesized during the EdU incubation period.
Fluorescent labelAlexa Fluor 350 picolyl azidePacific Blue picolyl azideAlexa Fluor 488 picolyl azideAlexa Fluor 594 picolyl azideAlexa Fluor 647 picolyl azide
Laser (nm)UV405488532 or 561633/635
Ex/Em (nm)350/438410/455495/519532 or 561/615650/668
Sample typeOptimized for live cells, the click detection step comes after the fixation.
Bibliography
Format50 assays50 assays50 assays100 assays50 assays50 assays100 assays
Cat. No. C10645C10636C10632C10633C10646C10634C10635

Limited multiplexability. Not compatible with R-PE, R-PE tandems and fluorescent proteins such as GFP and mCherry.
 

 Click-iT EdU Pacific Blue Flow Cytometry Kit Click-iT EdU Alexa Fluor 488 Flow Cytometry KitClick-iT EdU Alexa Fluor 647 Flow Cytometry Kit
Basis of assay
  • Incorporation of EdU into newly synthesized DNA
  • Detection using a fast, highly specific click reaction (Click-iT technology)
  • Labeled with photostable Alexa Fluor dyes
MultiplexableLimited multiplexability
  • Standard organic dyes (FITC, Alexa Fluor dyes, etc.)
  • Not compatible with R-PE, tandems, or fluorescent proteins
ReadoutFluorescent signal accumulates in the nuclei of cells where DNA has been synthesized during the EdU incubation period.
Fluorescent labelPacific Blue azideAlexa Fluor 488 azideAlexa Fluor 647 azide
Laser405 nm488 nm633/635 nm
Ex/Em (nm)410/455495/519650/668
Sample typeOptimized for live cells, the click detection step comes after the fixation.
Bibliography
Format50 assays50 assays100 assays50 assays100 assays
Cat. No. C10418C10424C10420C10425C10419

Ideal for maximum multiplexability in your flow cytometry experiments. Compatible with R-PE, R-PE tandems, and fluorescent proteins such as GFP and mCherry.
 

 Click-iT Plus EdU Alexa Fluor 350 Flow Cytometry KitClick-iT Plus EdU Pacific Blue Flow Cytometry Kit Click-iT Plus EdU Alexa Fluor 488 Flow Cytometry KitClick-iT Plus EdU Alexa Fluor 594 Flow Cytometry KitClick-iT Plus EdU Alexa Fluor 647 Flow Cytometry Kit
Basis of assay
  • Incorporation of EdU into newly synthesized DNA
  • Detection using a fast, highly specific click reaction of a picolyl azide (Click-iT Plus technology)
  • Labeled with photostable Alexa Fluor dyes or Pacific Blue
MultiplexableMaximum multiplexability
  • Standard organic dyes (FITC, Alexa Fluor dyes, etc.)
  • R-PE, R-PE tandems (such as R-PE-Cy5 or R-PE-Cy7)
  • Fluorescent proteins (such as GFP and mCherry)
  • Common cell staining methods
ReadoutFluorescent signal accumulates in the nuclei of cells where DNA has been synthesized during the EdU incubation period.
Fluorescent labelAlexa Fluor 350 picolyl azidePacific Blue picolyl azideAlexa Fluor 488 picolyl azideAlexa Fluor 594 picolyl azideAlexa Fluor 647 picolyl azide
Laser (nm)UV405488532 or 561633/635
Ex/Em (nm)350/438410/455495/519532 or 561/615650/668
Sample typeOptimized for live cells, the click detection step comes after the fixation.
Bibliography
Format50 assays50 assays50 assays100 assays50 assays50 assays100 assays
Cat. No. C10645C10636C10632C10633C10646C10634C10635

Limited multiplexability. Not compatible with R-PE, R-PE tandems and fluorescent proteins such as GFP and mCherry.
 

 Click-iT EdU Pacific Blue Flow Cytometry Kit Click-iT EdU Alexa Fluor 488 Flow Cytometry KitClick-iT EdU Alexa Fluor 647 Flow Cytometry Kit
Basis of assay
  • Incorporation of EdU into newly synthesized DNA
  • Detection using a fast, highly specific click reaction (Click-iT technology)
  • Labeled with photostable Alexa Fluor dyes
MultiplexableLimited multiplexability
  • Standard organic dyes (FITC, Alexa Fluor dyes, etc.)
  • Not compatible with R-PE, tandems, or fluorescent proteins
ReadoutFluorescent signal accumulates in the nuclei of cells where DNA has been synthesized during the EdU incubation period.
Fluorescent labelPacific Blue azideAlexa Fluor 488 azideAlexa Fluor 647 azide
Laser405 nm488 nm633/635 nm
Ex/Em (nm)410/455495/519650/668
Sample typeOptimized for live cells, the click detection step comes after the fixation.
Bibliography
Format50 assays50 assays100 assays50 assays100 assays
Cat. No. C10418C10424C10420C10425C10419


Click-iT EdU assays for imaging applications

Ideal for maximum multiplexability in imaging experiments. Compatible with fluorescent proteins such as GFP and mCherry.
 

 Click-iT Plus EdU Alexa Fluor 488 Imaging KitClick-iT Plus EdU Alexa Fluor 555 Imaging KitClick-iT Plus EdU Alexa Fluor 594 Imaging KitClick-iT Plus EdU Alexa Fluor 647 Imaging Kit
Basis of assay
  • Incorporation of EdU into newly synthesized DNA
  • Detection using a fast, highly specific click reaction of a picolyl azide (Click-iT Plus technology)
  • Labeled with photostable Alexa Fluor dyes
MultiplexableMaximum multiplexability
  • Does not destroy cellular epitopes (unlike BrdU protocols)
  • Compatible with:
    • Antibody labeling
    • Fluorescent proteins such as GFP and mCherry
    • Common cell or tissue staining methods for imaging
    • Standard organic dyes (FITC, Alexa Fluor dyes, etc.)
Readout
  • Fluorescent signal accumulates in the nuclei of cells where DNA has been synthesized during the EdU incubation period
Fluorescent labelAlexa Fluor 488 picolyl azideAlexa Fluor 555 picolyl azideAlexa Fluor 594 picolyl azideAlexa Fluor 647 picolyl azide
Standard filter setFITCTRITCTexas RedCy5
Ex/Em (nm)495/519555/565590/617650
Signal-to-noise ratio
Photostability
BibliographyCitations
ProtocolMicroscopy
Sample typeOptimized for live cells and tissue sections; the click detection step comes after fixation.
Format1 kit/50–250 coverslips1 kit/50–250 coverslips1 kit/50–250 coverslips1 kit/50–250 coverslips
Cat. No.C10637C10638C10639C10640

Limited multiplexability in imaging experiments. Not compatible with fluorescent proteins.
 

 Click-iT EdU Alexa Fluor 488 Imaging KitClick-iT EdU Alexa Fluor 555 Imaging KitClick-iT EdU Alexa Fluor 594 Imaging KitClick-iT EdU Alexa Fluor 647 Imaging Kit
Basis of assay
  • Incorporation of EdU into newly synthesized DNA
  • Detection using a fast, highly specific click reaction (Click-iT technology)
  • Labeled with photostable Alexa Fluor dyes
Multiplexable
  • Compatible with:
    • Common cell or tissue staining methods for imaging
    • Standard organic dyes (FITC, Alexa Fluor dyes, etc.)
  • Not compatible with fluorescent proteins
Readout
  • Fluorescent signal accumulates in the nuclei of cells where DNA has been synthesized during the EdU incubation period
Fluorescent labelAlexa Fluor 488 Alexa Fluor 555Alexa Fluor 594Alexa Fluor 647
Standard filter setFITCTRITCTexas RedCy5
Ex/Em (nm)495/519555/565590/617650/668
Signal-to-noise ratio
Photostability
BibliographyCitations
ProtocolMicroscopy
Sample typeOptimized for live cells; the click detection step comes after fixation.
Format1 kit/50–250 coverslips1 kit/50–250 coverslips1 kit/50–250 coverslips1 kit/50–250 coverslips
Cat. No.C10337C10338C10339C10340

Click-iT EdU kit designed and optimized for microplate readers.
 

 Click-iT EdU Proliferation Assay for Microplates
Basis of assay
  • Incorporation of EdU into newly synthesized DNA
  • Labeling via Click-iT technology with an HRP (horseradish peroxidase) moiety
  • Amplex UltraRed reagent is added and is converted to a highly fluorescent product
  • Fluorescence is recorded
ReadoutFluorescence of highly fluorescent product from reaction of Amplex UltraRed with HRP
Ex/Em (nm)568/585
Signal-to-noise ratio
ProtocolPlate reader
Sample typeOptimized for live cells, the click detection step comes after the fixation.
Format96-well plate
Cat. No.C10499

Click-iT EdU kits designed and optimized for high-content screen applications.
 

 Click-iT EdU Alexa Fluor 488 HCS AssayClick-iT EdU Alexa Fluor 555 HCS AssayClick-iT EdU Alexa Fluor 594 HCS AssayClick-iT EdU Alexa Fluor 647 HCS Assay
Basis of assay
  • Incorporation of EdU into newly synthesized DNA
  • Detection using a fast, highly specific click reaction (Click-iT technology)
  • Labeled with photostable Alexa Fluor dyes
Readout
  • Fluorescent signal accumulates in the nuclei of cells where DNA has been synthesized during the EdU incubation period
Fluorescent labelAlexa Fluor 488 Alexa Fluor 555Alexa Fluor 594Alexa Fluor 647
Standard filter setFITCTRITCTexas RedCy5
Ex/Em (nm)495/519555/565590/617650/668
Signal-to-noise ratio
Photostability
BibliographyCitations
ProtocolHCSMicroscopy
MultiplexingThe mild click chemistry protocol does not destroy cellular epitopes (unlike BrdU protocols) and is compatible with antibody labeling and common cell- or tissue-staining methods.
Sample typeOptimized for plate-based HCS assays. Reagents can be added directly to culture medium.
Format10 plates10 plates10 plates10 plates
Cat. No.C10351C10353C10355C10357

The Click-iT EdU Colorimetric IHC Detection Kit is perfect for tissues with high background fluorescence; the proliferation signal that can be archived for future analysis.
 

 Click-iT EdU Colorimetric IHC Detection Kit
Basis of assay
  • Incorporation of EdU into newly synthesized DNA
  • Labeling via Click-iT technology with a biotin moiety followed by biotin labeling with streptavidin-peroxidase
  • Upon the addition of a chromagen substrate, a dark brown signal results
  • Optimized for immunohistochemical detection of proliferating cells in tissue sections
Readout
  • The peroxidase enzyme and subsequent colorimetric precipitation accumulates in the nuclei of cells where DNA has been synthesized during the EdU incubation period
  • As little as 90 minutes
LabelColorimetric detection
PlatformLight microscopy
BibliographyCitations
ProtocolMicroscopy
MultiplexingCommonly used secondary tissue stains such as hematoxylin or methyl green can be used to review the cellular context surrounding the proliferation signal.
Sample typeOptimized for tissue sections
Format1 kit/50 slides
Cat. No.C10644

Ideal for maximum multiplexability in imaging experiments. Compatible with fluorescent proteins such as GFP and mCherry.
 

 Click-iT Plus EdU Alexa Fluor 488 Imaging KitClick-iT Plus EdU Alexa Fluor 555 Imaging KitClick-iT Plus EdU Alexa Fluor 594 Imaging KitClick-iT Plus EdU Alexa Fluor 647 Imaging Kit
Basis of assay
  • Incorporation of EdU into newly synthesized DNA
  • Detection using a fast, highly specific click reaction of a picolyl azide (Click-iT Plus technology)
  • Labeled with photostable Alexa Fluor dyes
MultiplexableMaximum multiplexability
  • Does not destroy cellular epitopes (unlike BrdU protocols)
  • Compatible with:
    • Antibody labeling
    • Fluorescent proteins such as GFP and mCherry
    • Common cell or tissue staining methods for imaging
    • Standard organic dyes (FITC, Alexa Fluor dyes, etc.)
Readout
  • Fluorescent signal accumulates in the nuclei of cells where DNA has been synthesized during the EdU incubation period
Fluorescent labelAlexa Fluor 488 picolyl azideAlexa Fluor 555 picolyl azideAlexa Fluor 594 picolyl azideAlexa Fluor 647 picolyl azide
Standard filter setFITCTRITCTexas RedCy5
Ex/Em (nm)495/519555/565590/617650
Signal-to-noise ratio
Photostability
BibliographyCitations
ProtocolMicroscopy
Sample typeOptimized for live cells and tissue sections; the click detection step comes after fixation.
Format1 kit/50–250 coverslips1 kit/50–250 coverslips1 kit/50–250 coverslips1 kit/50–250 coverslips
Cat. No.C10637C10638C10639C10640

Limited multiplexability in imaging experiments. Not compatible with fluorescent proteins.
 

 Click-iT EdU Alexa Fluor 488 Imaging KitClick-iT EdU Alexa Fluor 555 Imaging KitClick-iT EdU Alexa Fluor 594 Imaging KitClick-iT EdU Alexa Fluor 647 Imaging Kit
Basis of assay
  • Incorporation of EdU into newly synthesized DNA
  • Detection using a fast, highly specific click reaction (Click-iT technology)
  • Labeled with photostable Alexa Fluor dyes
Multiplexable
  • Compatible with:
    • Common cell or tissue staining methods for imaging
    • Standard organic dyes (FITC, Alexa Fluor dyes, etc.)
  • Not compatible with fluorescent proteins
Readout
  • Fluorescent signal accumulates in the nuclei of cells where DNA has been synthesized during the EdU incubation period
Fluorescent labelAlexa Fluor 488 Alexa Fluor 555Alexa Fluor 594Alexa Fluor 647
Standard filter setFITCTRITCTexas RedCy5
Ex/Em (nm)495/519555/565590/617650/668
Signal-to-noise ratio
Photostability
BibliographyCitations
ProtocolMicroscopy
Sample typeOptimized for live cells; the click detection step comes after fixation.
Format1 kit/50–250 coverslips1 kit/50–250 coverslips1 kit/50–250 coverslips1 kit/50–250 coverslips
Cat. No.C10337C10338C10339C10340

Click-iT EdU kit designed and optimized for microplate readers.
 

 Click-iT EdU Proliferation Assay for Microplates
Basis of assay
  • Incorporation of EdU into newly synthesized DNA
  • Labeling via Click-iT technology with an HRP (horseradish peroxidase) moiety
  • Amplex UltraRed reagent is added and is converted to a highly fluorescent product
  • Fluorescence is recorded
ReadoutFluorescence of highly fluorescent product from reaction of Amplex UltraRed with HRP
Ex/Em (nm)568/585
Signal-to-noise ratio
ProtocolPlate reader
Sample typeOptimized for live cells, the click detection step comes after the fixation.
Format96-well plate
Cat. No.C10499

Click-iT EdU kits designed and optimized for high-content screen applications.
 

 Click-iT EdU Alexa Fluor 488 HCS AssayClick-iT EdU Alexa Fluor 555 HCS AssayClick-iT EdU Alexa Fluor 594 HCS AssayClick-iT EdU Alexa Fluor 647 HCS Assay
Basis of assay
  • Incorporation of EdU into newly synthesized DNA
  • Detection using a fast, highly specific click reaction (Click-iT technology)
  • Labeled with photostable Alexa Fluor dyes
Readout
  • Fluorescent signal accumulates in the nuclei of cells where DNA has been synthesized during the EdU incubation period
Fluorescent labelAlexa Fluor 488 Alexa Fluor 555Alexa Fluor 594Alexa Fluor 647
Standard filter setFITCTRITCTexas RedCy5
Ex/Em (nm)495/519555/565590/617650/668
Signal-to-noise ratio
Photostability
BibliographyCitations
ProtocolHCSMicroscopy
MultiplexingThe mild click chemistry protocol does not destroy cellular epitopes (unlike BrdU protocols) and is compatible with antibody labeling and common cell- or tissue-staining methods.
Sample typeOptimized for plate-based HCS assays. Reagents can be added directly to culture medium.
Format10 plates10 plates10 plates10 plates
Cat. No.C10351C10353C10355C10357

The Click-iT EdU Colorimetric IHC Detection Kit is perfect for tissues with high background fluorescence; the proliferation signal that can be archived for future analysis.
 

 Click-iT EdU Colorimetric IHC Detection Kit
Basis of assay
  • Incorporation of EdU into newly synthesized DNA
  • Labeling via Click-iT technology with a biotin moiety followed by biotin labeling with streptavidin-peroxidase
  • Upon the addition of a chromagen substrate, a dark brown signal results
  • Optimized for immunohistochemical detection of proliferating cells in tissue sections
Readout
  • The peroxidase enzyme and subsequent colorimetric precipitation accumulates in the nuclei of cells where DNA has been synthesized during the EdU incubation period
  • As little as 90 minutes
LabelColorimetric detection
PlatformLight microscopy
BibliographyCitations
ProtocolMicroscopy
MultiplexingCommonly used secondary tissue stains such as hematoxylin or methyl green can be used to review the cellular context surrounding the proliferation signal.
Sample typeOptimized for tissue sections
Format1 kit/50 slides
Cat. No.C10644


The science behind EdU staining with Click-iT EdU technology

The Click-iT EdU technology advantage is in the chemistry—small, unique, and low-background labeling and detection moieties that react specifically and covalently with one another. 5-ethynyl-2´-deoxyuridine (EdU) is a nucleoside analog containing an alkyne. In a copper-catalyzed reaction, the alkyne reacts with a dye-labeled azide, forming a stable covalent bond. The small size of the azide reagent allows efficient access to the DNA without the need for harsh cell treatment. This simplifies the assay considerably, and the results you achieve are similar to (or better than) those typically achieved using BrdU (Figures 3 and 4).

3 panels. Panel 1 shows +/- Click-iT EdU populations, panel 2 shows most cells in the G0/G1 phase, and panel 3 shows standard horseshoe curve.


Figure 3. Cell proliferation analysis using the Click-iT Plus EdU Alexa Fluor 488 Flow Cytometry Assay Kit and FxCycle Violet Stain. Jurkat cells were treated with 10 μM EdU for one hour and stained with Invitrogen Alexa Fluor 488 picolyl azide, according to the protocol for the Invitrogen Click-iT Plus EdU Alexa Fluor 488 Flow Cytometry Assay Kit, followed by staining with Invitrogen FxCycle Violet Stain. Cells were then analyzed by flow cytometry using either 488 nm excitation (for Click-iT EdU Alexa Fluor 488 dye) or 405 nm excitation (for FxCycle Violet stain). (A) Histogram demonstrating clear separation of cells in S phase (DNA synthesis, including EdU incorporation) and cells in either G2/M or G0/G1(B) Histogram showing DNA content distribution, with G0/G1 and G2/M phase peaks separated by the S phase distribution using FxCycle Violet stain. (C) Dual parameter Click-iT Plus EdU and FxCycle plot shows co-positive cells that provide a direct measurement of the percentage of cells in S phase.

4 plots. Three plots show number of cells versus reagent fluorescence. Fourth plot shows standard dual-parameter horseshoe plot.

Figure 4. Alexa Fluor 488 Click-iT Plus EdU labeling with mCherry fluorescent protein and FxCycle Far Red stain. mCherry-expressing A549 cells were treated with 10 µM EdU for 2 hours and detected according to the recommended staining protocol. Panel A shows a histogram of cells labeled with Alexa Fluor 488 picolyl azide, identifying cells in S phase. Panels B and C show mCherry fluorescence from cells that were labeled with Click-iT Plus EdU Alexa Fluor 488 picolyl azide in the presence of copper (Panel B) and in the absence of copper (Panel C), demonstrating that the mCherry fluorescence is preserved in the click reaction. Panel D shows a dual parameter plot of Click-iT Plus EdU Alexa Fluor 488 and Invitrogen FxCycle Far Red stain for DNA content; cells that are co-positive are in S phase.

Maximum multiplexability with Click-iT Plus assays, overcoming trouble with copper

The copper concentrations typically used in traditional click chemistry reactions can affect fluorophores such as GFP, mCherry, R-PE, and R-PE tandem dyes. The Click-iT Plus formulation may be effectively employed in a low-copper reaction, enabling increased multiplexability compared to the original Click-iT EdU assays. Click-iT Plus EdU assays can be used in conjunction with R-PE and R-PE tandems, as well as fluorescent proteins such as GFP and mCherry, without loss of the accuracy or speed of the original Click-iT EdU assay. The low-copper reaction with Click-iT Plus EdU results in bright signals and retains the fluorescent signal from GFP (Figure 5). In addition, detection of EdU with the Click-iT Plus Alexa Fluor 488 picolyl azide was compatible with PE-Cy7 fluorescence in a flow cytometric assay for cell proliferation (Figure 6). Select from Click-iT Plus EdU kits for flow cytometry or Click-iT Plus EdU assays for imaging applications.

Multiplex with:

  • Most standard antibody conjugates available
  • Sensitive R-PE fluorophores such as R-PE-Cy7
  • GFP, mCherry, and other fluorescent proteins
  • Cell surface and intracellular markers
  • Cell cycle dyes

Click-iT EdU Plus for flow cytometry: Enhanced compatibility for multiplexing

Figure 6. Results from immunophenotyping experiment to evaluate CD3 and DNA strand breaks in human T cells. Dual parameter plot of Click-iT Plus EdU Alexa Fluor 488 Flow Cytometry Assay Kit and Hu CD3 PE-Cy7 fluorescence.

Dot plot. 1 population positive for CD3 but negative for Click-iT EdU fluorescence and 1 population co-positive for both.
Promotional image for Immunology at Work Resource Center

Immunology at work

Learn immune cell type markers and protocols. The Invitrogen Immunology at Work Resource Center is a learning center with technical content designed for new and experienced life scientists alike exploring the field of immunology.

Get started on your experiments

Resources

Tools

Flow Cytometry Panel Builder—Design your flow cytometry panel with this online tool for a simplified, customizable experience to fit your needs.

Fluorescence SpectraViewer—Online tool for visualization of the excitation and emission of fluorescent reagents. Tool allows for checking spectral compatibility for multiple fluorophores.

Cell viability, proliferation, and cell cycle information—Find educational resources such as application notes, webinars, videos, articles, and more that cover the use of many of our reagents and kits for monitoring cell function.

Support

Cell Analysis Support Center—Find technical information, tips and tricks, and answers to everyday problems.

BioProbes Journal of Cell Biology Applications Subscribe—Stay up to date with highlights of the latest breakthroughs and get information about new technologies and products.