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Invitrogen
BrdU Monoclonal Antibody (MoBU-1), Alexa Fluor™ 488
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FIGURE: 1 / 23
BrdU Antibody (B35130) in ICC/IF
Aging cell 2021 -
FIGURE 1 Deep senescence (DS) in primary adult human mammary fibroblasts is reversible. (a) Schematic illustrating epithelial and fibroblast senescence and the DS reversal strategy. (b) Early proliferating (EP) fibroblasts at P11 were serially passaged until they reached senescence at P29. Deeply senescent (DS) fibroblasts were defined as a population which did not expand when kept in culture for 3 weeks po... View More
Product Details
B35130
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Published species
Chemical,
Human,
Mouse
Host/Isotype
Mouse
/ IgG
Class
Monoclonal
Type
Antibody
Clone
MoBU-1
Immunogen
BrdU
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
5mM sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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