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          • Primary Antibodies ›
          • BrdU Antibodies

          Invitrogen

          BrdU Monoclonal Antibody (MoBU-1), Alexa Fluor™ 594

          1 Published Figure
          11 References
          View all (92) BrdU antibodies

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          Cite BrdU Monoclonal Antibody (MoBU-1), Alexa Fluor™ 594

          Additional Information:
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          • Published Figures (1)
          BrdU Antibody in Immunohistochemistry (IHC)
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          BrdU Antibody in Immunohistochemistry (IHC)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          BrdU Antibody (B35132) in IHC

          Development (Cambridge, England) 2013 - Published figure using BrdU monoclonal antibody (Product # B35132) in Immunohistochemistry {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          BrdU Antibody in Immunohistochemistry (IHC)

          Product Details

          B35132

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (IHC)

          Assay-dependent
          View 2 publications 2 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          -
          View 7 publications 7 publications

          Immunoprecipitation (IP)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Chemical

          Published species

          Chemical, Mouse

          Host/Isotype

          Mouse / IgG

          Class

          Monoclonal

          Type

          Antibody

          Clone

          MoBU-1

          Immunogen

          BrdU

          Conjugate

          Alexa Fluor™ 594 Alexa Fluor™ 594 Alexa Fluor™ 594
          • Unconjugated
          • Alexa Fluor 488 
          • Alexa Fluor 647 
          • Biotin 
          • Pacific Blue
          • Request custom conjugation

          Excitation/Emission Max

          590/618 nm View spectra spectra

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Amount

          70 µg

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          5mM sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2536436

          Target Information

          Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: 5-Bromo-2-deoxyuridine; Bromodeoxyuridine

          View more View less

          It has to be done as per old AB suggested Products section.

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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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