Articles that detail flow cytometry applications

The articles listed below comprise our application notes, scientific posters, and BioProbes™ Journal articles collections. These resources give overviews of specific flow cytometry applications and as well as experimental design details and data and results. 


Featured articles

Copper-Safe Click-iT Plus EdU Proliferation Assay: Improved Compatibility with Simultaneous Phycobiliprotein and Fluorescent Protein Detection

This scientific poster demonstrates how the Click-iT Plus EdU Proliferation assay can be used with classic fluorescent proteins and phycobiliproteins for cell proliferation detection, eliminating the need for using cumbersome antibody-based Anti-BrdU assays.

Multiparameter analysis of murine regulatory T cells and dendritic cells with the Attune NxT Flow Cytometer

This application note describes the use of the Attune NxT Flow Cytometer for 4-laser and 10-color immunophenotyping analysis of stained mouse splenocytes using a fixation and permeabilization protocol.


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Type Title Category
Application note (2011) Apoptosis detection: Apoptosis assays for the Attune Acoustic Focusing Cytometer Alexa Fluor, apoptosis, Attune/Attune NxT, cell structure-mitochondria, cell structure-plasma membrane, flow cytometer/flow cytometry, fluorescent dyes
Application note (2011) Bacterial analysis using the Attune Acoustic Focusing Cytometer Attune/Attune NxT, flow cytometer/flow cytometry, fluorescent dyes, microbiology, viability
Application note (2011) Detecting green fluorescent protein using the Attune Acoustic Focusing Cytometer Attune/Attune NxT, BacMam technology, CellLight, flow cytometer/flow cytometry, fluorescent proteins, protein detection, protein quantitation
Application note (2011) Detecting red fluorescent protein using the Attune Acoustic Focusing Cytometer Attune/Attune NxT, BacMam technology, CellLight, flow cytometer/flow cytometry, fluorescent proteins, protein detection, protein quantitation
Application note (2011) Detection of human mesenchymal stem cells using the Attune Acoustic Focusing Cytometer Attune/Attune NxT, cell cycle, flow cytometer/flow cytometry, immunophenotyping, stem cell research
Application note (2011) Detection of phosphoproteins using the Attune Acoustic Focusing Cytometer Alexa Fluor, antibodies, Attune/Attune NxT, cell signaling, cells, flow cytometer/flow cytometry, fluorescent dyes, protein detection
Application note (2011) Immunophenotyping peripheral blood cells: A no-lyse, no-wash, no cell loss method for immunophenotyping nucleated peripheral blood cells using the Attune Acoustic Focusing Cytometer antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, flow cytometry sample preparation, fluorescent dyes, immunophenotyping, particles
Application note (2011) Mouse immunophenotyping: A no-lyse, no-wash, and no cell loss method using the Attune Acoustic Focusing Cytometer antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, flow cytometry sample preparation, fluorescent dyes, immunophenotyping, particles
Application note (2011) Novel detection and direct cell counting of marine, photosynthetic picoplankton by flow cytometry Attune/Attune NxT, cell counting, flow cytometer/flow cytometry, flow cytometry sample preparation
Application note (2011) Six-color mouse lymphocyte immunophenotyping using the Attune Acoustic Focusing Cytometer with red laser option Alexa Fluor, antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, multicolor flow cytometry, particles
Application note (2012) Detecting human circulating endothelial cells using the Attune Acoustic Focusing Cytometer Alexa Fluor, antibodies, Attune/Attune NxT, cells, endothelial cells, flow cytometer/flow cytometry, fluorescent dyes, rare-event detection
Application note (2012) Microbiological applications using the Attune Acoustic Focusing Cytometer Attune/Attune NxT, cell health, flow cytometer/flow cytometry, fluorescent dyes, microbiology, viability
Application note (2015) 13-parameter immunophenotyping of human lysed whole blood with the the Attune NxT Flow Cytometer allows for the identification of B cells, NK cells, multiple T cell subsets and myeloid cells Alexa Fluor, antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, fluorescent dyes, immune system, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot
Application note (2015) Attune NxT Flow Cytometer for 6-Color immunophenotyping analysis of stained human whole blood using a no-lyse, no-wash protocol, with no compensation Attune/Attune NxT, flow cytometer/flow cytometry, immunophenotyping, multicolor flow cytometry
Application note (2015) Detection of murine regulatory T cells on the Attune NxT Flow Cytometer Attune/Attune NxT, flow cytometer/flow cytometry, immunophenotyping, multicolor flow cytometry
Application note (2015) Live/dead algal analysis with the Attune NxT Flow Cytometer algae, Attune/Attune NxT, flow cytometer/flow cytometry, fluorescent dyes, viability
Application note (2015) Multiparameter analysis of murine regulatory T cells and dendritic cells with the Attune NxT Flow Cytometer Attune/Attune NxT, flow cytometer/flow cytometry, immunophenotyping, multicolor flow cytometry
Application note (2015) No-wash, no-lyse detection of phagocytic cells via a pHrodo BioParticles functional assay in human whole blood on the Attune NxT Flow Cytometer antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, flow cytometry sample preparation, fluorescent dyes, immune system, immunophenotyping, particles, pH detection
Application note (2015) Ten-parameter immunophenotyping of human lysed whole blood with the Attune NxT Flow Cytometer allows identification of multiple T cell subsets and myeloid cells Attune/Attune NxT, flow cytometer/flow cytometry, immunophenotyping, multicolor flow cytometry
BioProbes article (2006) A guide to antibody labeling and detection Alexa Fluor, antibody labeling, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, multicolor flow cytometry, nanocrystals, Qdot, Zenon
BioProbes article (2006) Analyzing cell culture health and concentration apoptosis, cell health, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, microplate reader, particles, viability
BioProbes article (2006) Invitrogen flow cytometry—building choice cell culture, cell cycle, cell health, cell isolation, cell signaling, flow cytometer/flow cytometry, fluorescent dyes, Gibco, fluorescence microscopy/fluorescence imaging, nanocrystals, protein labeling, Qdot, Zenon
BioProbes article (2006) Multicolor immunodetection using the violet laser Alexa Fluor, antibody labeling, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot
BioProbes article (2006) Products for neurobiology research enzyme activity detection, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, in situ hybridization, nanocrystals, protein detection, protein labeling, Qdot, viability, Zenon
BioProbes article (2006) Tools for membrane research cell structure-mitochondria, cell structure-plasma membrane, cell tracking, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, lipids
BioProbes article (2007) A new approach to cell-based multiplexing expands the drug screening capabilities of flow cytometry Alexa Fluor, drug discovery, flow cytometer/flow cytometry, fluorescent dyes, high content analysis, sample preparation
BioProbes article (2007) Breakthrough cell proliferation assay for flow cytometry Alexa Fluor, cell proliferation, Click-iT, drug discovery, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, nucleic acid quantitation
BioProbes article (2007) Cells from the inside out cell structure-all, flow cytometer/flow cytometry, fluorescent proteins, fluorescence microscopy/fluorescence imaging, in vivo imaging, multicolor flow cytometry, nanocrystals, Qdot
BioProbes article (2007) Dead-cell discrimination in a different light flow cytometer/flow cytometry, fluorescent dyes, viability,
BioProbes article (2007) Identifying Alzheimer's diease biomarkers from human brain tissue flow cytometer/flow cytometry, fluorescent dyes, Luminex, multiplex ELISA/Luminex, neuroscience, particles, protein detection, protein quantitation, proteins, sample preparation
BioProbes article (2007) Important additions to the Alexa Fluor dye series Alexa Fluor, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, protein labeling, sample stability
BioProbes article (2007) Improved results are just a click away Alexa Fluor, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, in vivo imaging, multicolor flow cytometry, nucleic acid quantitation, nucleosides
BioProbes article (2007) Investigating apoptosis-induced loss of SYTO dye fluorescence apoptosis, cell structure-mitochondria, enzyme activity detection, flow cytometer/flow cytometry, fluorescent dyes
BioProbes article (2007) Looking for apoptosis? antibodies, apoptosis, colorimetric, ELISA, flow cytometer/flow cytometry, fluorescence, fluorescent dyes, immunocytochemistry (ICC), protein detection, western blotting
BioProbes article (2007) Mercator multiplex phosphoantibody-based array antibody labeling, flow cytometer/flow cytometry, fluorescent dyes, high content analysis, protein labeling, protein quantitation
BioProbes article (2007) Multiparametric approaches for studying apoptosis Alexa Fluor, antibodies, apoptosis, cell structure-mitochondria, cell structure-plasma membrane, enzyme activity detection, flow cytometer/flow cytometry, fluorescent dyes, viability
BioProbes article (2007) New applications for Vybrant DyeCycle stains Alexa Fluor, cell cycle, cell isolation, drug discovery, flow cytometer/flow cytometry, fluorescent dyes, high content analysis, live-cell imaging, multicolor flow cytometry, stem cell research
BioProbes article (2007) Putting the pH in phagocytosis flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, microplate reader, multicolor flow cytometry, pH detection, phagocytosis, reagents, sample preparation
BioProbes article (2007) Quantum dots as replacements for tandem dyes in flow cytometry flow cytometer/flow cytometry, fluorescent dyes, multicolor flow cytometry, nanocrystals, protein labeling, Qdot
BioProbes article (2007) Simultaneously visualize surface and intracellular markers antibody labeling, cell fixatives, flow cytometer/flow cytometry, fluorescent dyes, sample preparation, Zenon
BioProbes article (2007) Streptavidin conjugates of R-phycoerythrin—detection workhorses flow cytometer/flow cytometry, fluorescent proteins, Luminex, microarrays, multiplex ELISA/Luminex, particles, protein labeling
BioProbes article (2007) The latest in stem cell research antibodies, antibody labeling, cell differentiation, cell expansion, flow cytometer/flow cytometry, fluorescent dyes, growth factors, fluorescence microscopy/fluorescence imaging, stem cell research
BioProbes article (2007) The use of Qdot nanocrystals in flow cytometry flow cytometer/flow cytometry, fluorescent dyes, multicolor flow cytometry, nanocrystals, protein labeling, Qdot, viability
BioProbes article (2008) A cell-based approach for interrogating the PI3K/AKT/mTOR pathway—CellSensor and LanthaScreen assay cell signaling, enzyme activity detection, flow cytometer/flow cytometry, fluorescent proteins, western blotting
BioProbes article (2008) A no-wash indicator for high-throughput calcium detection BacMam technology, cell signaling, drug discovery, flow cytometer/flow cytometry, fluorescent proteins, high content analysis, ion flux/ion channels, ion indicators, microplate reader
BioProbes article (2008) Click chemistry: Making biodiscovery a snap Alexa Fluor, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, in vivo imaging, nucleosides
BioProbes article (2008) Faster, more flexible protein quantitation colorimetric, flow cytometer/flow cytometry, fluorescence, fluorescent dyes, Luminex, multiplex ELISA/Luminex, particles, protein quantitation
BioProbes article (2008) Flow cytometry: checking vital signs Alexa Fluor, cell health, cell proliferation, CellTrace, Click-iT, enzyme activity detection, flow cytometer/flow cytometry, fluorescent dyes, nucleic acid quantitation, viability
BioProbes article (2008) IgG antibody labeling from A to Z—Alexa Fluor to Zenon IgG Antibody Labeling Kits for imaging and flow cytometry Alexa Fluor, antibody labeling, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, protein labeling, Zenon
BioProbes article (2008) Journal Club—Getting a head start of Alzheimer's disease detection flow cytometer/flow cytometry, fluorescent dyes, Luminex, multiplex ELISA/Luminex, neuroscience, particles, protein detection, proteins
BioProbes article (2008) Putting regulatory T cells to work—Isolate and expand CD4+ CD25+ Treg cells using Dynabeads magnetic separation technology cell expansion, cell isolation, flow cytometer/flow cytometry, fluorescent dyes, immune system, magnetic beads, reagents
BioProbes article (2008) Qdot 625 nanocrystals and conjugates flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, multicolor flow cytometry, nanocrystals, Qdot
BioProbes article (2008) Seeing green—Alexa Fluor 488 conjugates outshine the rest Alexa Fluor, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, sample stability
BioProbes article (2008) Single-step isolation of functional human NK cells antibodies, cell isolation, flow cytometer/flow cytometry, fluorescent dyes, immune system, magnetic beads, sample preparation
BioProbes article (2008) The picture of cell health Alexa Fluor, cell health, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, microplate reader, nucleic acid quantitation, nucleosides, reagents
BioProbes article (2008) Using Qdot nanocrystal primary antibody conjugates in flow cytometry flow cytometer/flow cytometry, fluorescent dyes, multicolor flow cytometry, nanocrystals, Qdot, sample preparation, set up
BioProbes article (2009) A flow cytometry–based method for isolating neural stem cells Alexa Fluor, cell isolation, flow cytometer/flow cytometry, fluorescent dyes, neuroscience, stem cell research
BioProbes article (2009) A fluorogenic, target-specific reagent for small animal in vivo imaging Alexa Fluor, antibody labeling, cancer, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, in vivo imaging, protein labeling, sample preparation
BioProbes article (2009) A growing list of tools for the violet laser Alexa Fluor, apoptosis, cell cycle, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, nanocrystals, Qdot, viability
BioProbes article (2009) Accurate compensation is as easy as AbC calibration, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, multicolor flow cytometry, particles
BioProbes article (2009) Beyond Classification—Click-iT EDU outperforms BRDU across all species Alexa Fluor, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, nucleic acid detection, nucleic acid quantitation, nucleosides, sample preparation
BioProbes article (2009) Dead cell stains in flow cytometry: A comprehensive analysis apoptosis, flow cytometer/flow cytometry, fluorescent dyes, multicolor flow cytometry, viability
BioProbes article (2009) Dynamic signaling by integrins cancer, cell signaling, Cellular Lights, ELISA, flow cytometer/flow cytometry, fluorescent proteins, proteins, western blotting
BioProbes article (2009) Fluorescence technologies for high-content imaging and analysis Alexa Fluor, cell health, Click-iT, drug discovery, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, high content analysis, oxidative stress, protein detection, viability
BioProbes article (2009) Is it time to go direct? IgG antibody labeling methods as versatile as your imagination Alexa Fluor, antibody labeling, flow cytometer/flow cytometry, fluorescent dyes, immunocytochemistry (ICC), immunohistochemistry (IHC), nanocrystals, Qdot, sample preparation, Zenon
BioProbes article (2009) Isolate T cells directly from whole blood cell isolation, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, immune system, magnetic beads
BioProbes article (2009) Isolation and expansion of mouse Treg cells using Dynabeads technology Alexa Fluor, cell expansion, cell isolation, flow cytometer/flow cytometry, fluorescent dyes, immune system, magnetic beads
BioProbes article (2009) Live-cell imaging of cell cycle and cell division BacMam technology, cell cycle, flow cytometer/flow cytometry, fluorescent proteins,  fluorescence microscopy/fluorescence imaging, high content analysis, live-cell imaging
BioProbes article (2009) Mitochondrial fission and fusion in neurodegeneration antibodies, BacMam technology, cell structure-mitochondria, flow cytometer/flow cytometry, fluorescent dyes, fluorescent proteins, fluorescence microscopy/fluorescence imaging, microplate reader, neuroscience
BioProbes article (2009) Multiparametric approaches for predictive hepatotoxicity cell structure-mitochondria, cell structure-plasma membrane, flow cytometer/flow cytometry, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, oxidative stress, viability
BioProbes article (2009) Nanotechnology meets cellular analysis cell structure-all, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, media, multicolor flow cytometry, nanocrystals, Qdot, sample stability
BioProbes article (2009) Near-infrared dye for live cell cycle analysis cell cycle, flow cytometer/flow cytometry, fluorescent dyes, live-cell imaging, nucleic acid detection
BioProbes article (2009) Novel recombinant monoclonal antibodies—ABfinity technology ensures performance antibodies, cell signaling, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC), protein quantitation, sample preparation, western blotting
BioProbes article (2009) Novel solid-phase antibody labeling Alexa Fluor, antibody labeling, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, sample preparation, Zenon
BioProbes article (2009) Resources for cytokine research antibodies, cell signaling, chemiluminescence, ELISA, flow cytometer/flow cytometry, fluorescence, Luminex, multiplex ELISA/Luminex, particles, protein detection, protein quantitation, proteins,
BioProbes article (2009) Staining DNA without altering its structure cell structure-nucleus, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, live-cell imaging, nucleic acid detection
BioProbes article (2009) Targeting cardiovascular disease and Alzheimer’s disease cardiovascular system, drug discovery, flow cytometer/flow cytometry, fluorescent dyes, microplate reader, neuroscience, particles
BioProbes article (2009) The envelope, please: Best picture for biofilms biofilm staining, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, microbiology, microplate reader, viability
BioProbes article (2009) Tools for the study of Alzheimer’s disease antibodies, ELISA, flow cytometer/flow cytometry, Luminex, multiplex ELISA/Luminex, neuroscience, particles, protein detection, protein quantitation, proteins
BioProbes article (2009) Traffic lights: Illuminating internalization pathways drug discovery, flow cytometer/flow cytometry, fluorescent dyes, particles, pH detection, phagocytosis, reagents
BioProbes article (2010) A ratiometric approach to apoptosis detection by flow cytometry apoptosis flow cytometer/flow cytometry, fluorescence
BioProbes article (2010) Advanced cell proliferation analysis Attune/Attune NxT cell proliferation, cell tracking, flow cytometer/flow cytometry, fluorescence
BioProbes article (2010) Dead cell identification in flow cytometry flow cytometer/flow cytometry fluorescence, viability
BioProbes article (2010) Optimal pairing of dyes and antibodies for flow cytometry antibodies, flow cytometer/flow cytometry, fluorescence, multicolor flow cytometry, particles Qdot
BioProbes article (2010) Tuning acoustic forces for flow cytometry Attune/Attune NxT flow cytometer/flow cytometry, fluorescence
BioProbes article (2011) A no-lyse, no-wash, no–cell loss method for immunophenotyping—Simplify sample preparation without compromising data using the Attune Attune/Attune NxT flow cytometer/flow cytometry, flow cytometry sample preparation, fluorescence, immunophenotyping
BioProbes article (2011) Accurate assessment of microbial viability by flow cytometry—LIVE/DEAD BacLight Bacterial Viability Kit meets the Attune Acoustic Focusing Cytometer Attune/Attune NxT, flow cytometer/flow cytometry fluorescence, microbiology, viability
BioProbes article (2011) Click-iT EdU Flow Cytometry Kits— Simplifying cell proliferation analysis cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence
BioProbes article (2011) Flow cytometry in environmental microbiology—Using the Attune Flow Cytometer to detect marine photosynthetic picoplankton Attune/Attune NxT, flow cytometer/flow cytometry, fluorescence, microbiology
BioProbes article (2011) Rare stem cells detected using the Attune Acoustic Focusing Cytometer Attune/Attune NxT, flow cytometer/flow cytometry, fluorescence, stem cell research
BioProbes article (2011) Tracking cells through time and space—Choose from fluorescent Dyes, Qdot nanocrystals, and targeted fluorescent proteins CellTrace, cell tracking, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent proteins,  live cells
BioProbes article (2012) A new application for pHrodo dextrans antibodies, antibody labeling, Click-iT, flow cytometer/flow cytometry, fluorescence particles, protein labeling, Qdot
BioProbes article (2012) Detecting rare events—Identify circulating endothelial cells with the Attune Acoustic Focusing Cytometer Attune/Attune NxT, flow cytometer/flow cytometry, fluorescence
BioProbes article (2012) Expanding the fluorescence toolbox—Improved Qdot probes make multiplexing even easier flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, multicolor flow cytometry, particles, Qdot
BioProbes article (2012) Tracking internalization in live cells with pHrodo dyes endocytosis/phagocytosis, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, Internalization, pHrodo
BioProbes article (2012) Unmasking cell replication—Reagents for analyzing cell cycle and replication in mammalian cells cell cycle, cell health cell proliferation, cell structure-nucleus, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging
BioProbes article (2012) Your flow experiments just got faster Attune/Attune NxT, autosampler, flow cytometer/flow cytometry fluorescence
BioProbes article (2013) Checking vital signs: Don't let dead cells mislead you—Molecular Probes cell health assays for flow cytometry apoptosis, caspase substrate, cell cycle, cell health, cell proliferation, cell tracking, Click-iT, flow cytometer/flow cytometry, fluorescence, oxidative stress, viability,
BioProbes article (2013) Expand your multicolor capacity with the Pacific Green dye—New violet laser–excitable Pacific Green dye and conjugates for flow cytometry flow cytometer/flow cytometry, immunophenotyping, violet-laser excited dyes and reagents
BioProbes article (2013) Oxidative stress from a cell's point of view—From CellROX ROS sensors to Image-iT and Click-iT lipid peroxidation detection kits CellROX, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, oxidative stress
BioProbes article (2014) Detect pH inside of a live cell—pHrodo pH sensors for detecting cytosolic and vesicle pH flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, microplate reader, flow cytometer/flow cytometry, pHrodo
BioProbes article (2014) Multiplex your cell proliferation assay with GFP, RFP, and R-PE probes—Click-iT Plus EdU Proliferation Kits for imaging and flow cytometry cell proliferation, Click-iT, flow cytometer/flow cytometry, cell proliferation, multicolor flow cytometry
BioProbes article (2014) Obtain the highest-quality data your system can deliver—Fluorescent microspheres for calibrating microscopes and flow cytometers calibration, cell counting, flow cytometer/flow cytometry, flow cytometer set up and calibration, fluorescence microscopy/fluorescence imaging, immunophenotyping, microscope calibration, particles
BioProbes article (2014) Superior live-cell tracing, generation after generation—The CellTrace Far Red Cell Proliferation Kit cell proliferation, CellTrace, flow cytometer/flow cytometry, fluorescence
BioProbes article (2014) The next generation in acoustic focusing cytometry is here—The Attune NxT Acoustic Focusing Cytometer antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, fluorescence
BioProbes article (2015) Flow cytometry panel design: The basics Attune/Attune NxT, flow cytometer/flow cytometry, fluorescence, immunophenotyping, multicolor flow cytometry
BioProbes article (2015) Isolation and characterization of exosomes using magnetic beads antibodies, exosomes, flow cytometer/flow cytometry, fluorescence, immunophenotyping, multiplex ELISA/Luminex,  particles, protein blotting, western detection
BioProbes article (2015) Journal Club—Multiparameter detection of early apoptosis markers without compensation apoptosis, Attune/Attune NxT, cell structure-mitochondria, flow cytometer/flow cytometry fluorescence, immunophenotyping
BioProbes article (2015) Multiparameter detection of fluorescent protein expression with the Attune NxT Flow Cytometer Attune/Attune NxT, flow cytometer/flow cytometry, fluorescence, fluorescent proteins
BioProbes article (2015) No-compensation immunophenotyping using a four-laser flow cytometer Attune/Attune NxT, flow cytometer/flow cytometry, fluorescence, immunophenotyping, multicolor flow cytometry
BioProbes article (2015) No-wash, no-lyse assays for the Attune NxT Flow Cytometer Attune/Attune NxT, flow cytometer/flow cytometry, fluorescence, immunophenotyping, multicolor flow cytometry
BioProbes article (2015) The next generation in flow cytometry—The Attune NxT Flow Cytometer antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, fluorescence
BioProbes article (2015) Tools and strategies for rare-event detection using flow cytometry Attune/Attune NxT, flow cytometer/flow cytometry, fluorescence, rare cell detection
BioProbes article (2016) Accurately measure cell concentrations by flow cytometry Attune/Attune NxT, cell concentration, flow cytometer/flow cytometry, microbiology, viability
BioProbes article (2016) Tracking cell division with flow cytometry CellTrace, cell cycle, cell proliferation, cell tracking, flow cytometer/flow cytometry
Scientific poster (2006) Novel violet-excited reagents for detection of viability and vitality  Alexa Fluor, cell health, CellTrace, flow cytometer/flow cytometry, fluorescent dyes, viability, violet laser-excited reagents
Scientific poster (2007) A new approach for the detection of intracellular glutathione by fluorescence microscopy and flow cytometry ArrayScan, cells, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, live-cell imaging, microplate reader, viability, violet laser-excited reagents
Scientific poster (2007) Evaluation of click chemistry-based alternative to BrdU antibody labeling in tissue and cultured cells using fluorescence microscopy and flow cytometry antibodies, ArrayScan, cell cycle, cell proliferation, cells, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, microplate reader, nucleic acid labeling
Scientific poster (2008) A new 405 nm excitable fluorescent probe of intracellular thiols for imaging and flow cytometric assays ArrayScan, cells, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, microplate reader, violet laser-excited reagents
Scientific poster (2008) Characterization of DNA content, cyclin B1 and phosphorylated histone H3 with direct S-phase using EDU incorporation in multiparameter testing of cell lines with cell cycle blocking agents Alexa Fluor, antibodies, apoptosis, ArrayScan, cell cycle, cell proliferation, cells, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Scientific poster (2008) Detection of DNA synthesis by automated microscopy and image analysis: Comparison of BRDU method and a new click chemistry-based EDU method Alexa Fluor, antibodies, ArrayScan, cell cycle, cell proliferation, cells, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, microplate reader, nucleic acid labeling
Scientific poster (2008) Detection of S-phase cell cycle progression using 5-ethynyl-2'-deoxyuridine incorporation with click chemistry Alexa Fluor, antibodies, ArrayScan, cell cycle, cells, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, microplate reader, nucleic acid labeling, nucleic acid quantitation
Scientific poster (2008) Effects of the thymidine analogues EdU and BrdU on cell viability and cycle progression Alexa Fluor, cell cycle, cell proliferation, cells, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, viability
Scientific poster (2008) Zenon labeling technology facilitates detection of intra-cellular phosphoprotein determinants Alexa Fluor, antibodies, cell signaling, cells, flow cytometer/flow cytometry, fluorescent dyes, protein labeling, rare cell detection, Zenon
Scientific poster (2009) A comparative study of 25 different CD4 conjugates Alexa Fluor, antibodies, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot
Scientific poster (2009) A novel xeno-free and serum-free culture medium for the expansion of human mesenchymal stem cells cell culture/expansion, cells, flow cytometer/flow cytometry, fluorescent dyes, gene expression, stem cell research
Scientific poster (2009) A simple, fast and quantitative single-step dead-cell indicator for flow cytometry apoptosis, flow cytometer/flow cytometry, fluorescent dyes, multicolor flow cytometry, nucleic acid quantitation, viability
Scientific poster (2009) Dead cell stains in flow cytometry: A comprehensive analysis apoptosis, cells, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, viability
Scientific poster (2009) Dual pulse labeling of S-phase population using click chemistry to measure changes in cell proliferation Alexa Fluor, cancer, cell cycle, cell proliferation, cells, Click-iT, flow cytometer/flow cytometry, fluorescent dyes
Scientific poster (2009) Dual pulse labeling using a new thymidine analog 5-ethynyl-2’-deoxyuridine (EdU) to detect alterations in S-phase progression by fluorescence microscopy and flow cytometry Alexa Fluor, cell cycle, cell proliferation, cells, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Scientific poster (2009) Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry Alexa Fluor, cell proliferation, cells, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, nucleic acid quantitation, stem cell research, violet laser-excited reagents
Scientific poster (2009) Qdot nanocrystal conjugates in multispectral flow cytometry Alexa Fluor, antibodies, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot, violet laser-excited reagents
Scientific poster (2010) A comparison of three techniques to induce efficient ex vivo T cell expansion Alexa Fluor, antibodies, cell proliferation, CellTrace, flow cytometer/flow cytometry, fluorescent dyes, immune system, magnetic beads, multicolor flow cytometry, viability
Scientific poster (2010) Acoustic focusing cytometry: Sensitivity and throughput apoptosis, Attune/Attune NxT, cell cycle, cells, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, phagocytosis
Scientific poster (2010) A universal click chemistry-based enrichment column for the identification of multiple subclasses of PTM-modified proteins and newly synthesized proteins Alexa Fluor, cells, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, protein detection, protein enrichment, protein labeling
Scientific poster (2010) Abfinity antibodies: A novel approach to antibody development Alexa Fluor, antibodies, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gene expression, immunocytochemistry (ICC), western blotting
Scientific poster (2010) Acoustic flow cytometry for multicolor immunophenotyping analysis antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, fluorescent dyes, immune system, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot, violet laser-excited reagents
Scientific poster (2010) Analysis of proliferation dynamics in primary and immortalized human cells Alexa Fluor, cancer, cell proliferation, CellTrace, circulatory system, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, fluorescent proteins, nucleic acid quantitation
Scientific poster (2010) Dual pulse labeling using EdU and BrdU to monitor alterations in cellular proliferation by fluorescence microscopy and flow cytometry Alexa Fluor, cell cycle, cell proliferation, cells, Click-iT, drug discovery, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Scientific poster (2010) Functional characterization of a novel fluorescent dye for proliferation analysis Alexa Fluor, cell proliferation, cells, CellTrace, flow cytometer/flow cytometry, fluorescent dyes, violet laser-excited reagents
Scientific poster (2010) New fluorescent probes for live-cell imaging autophagy, cell cycle, CellLight, cells, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent proteins, live-cell imaging, pH detection, phagocytosis
Scientific poster (2010) Non-cytotoxic near-IR DNA stain for cell cycle analysis in living cells cell cycle, cells, flow cytometer/flow cytometry, fluorescent dyes, fluorescent proteins, live-cell imaging, nucleic acid labeling, viability
Scientific poster (2010) Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry and imaging Alexa Fluor, cell proliferation, cells, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, immunofluorescence (IF), immunophenotyping, stem cell research, violet laser-excited reagents
Scientific poster (2010) Rare event detection using acoustic cytometry Attune/Attune NxT, cells, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, rare-event detection
Scientific poster (2010) Simultaneous analysis of cell death mechanisms and oxidative stress using Molecular Probes next generation reagents for imaging and flow cytometry apoptosis, autophagy, caspase substrates, cells, flow cytometry/flow cytometer, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, oxidative stress
Scientific poster (2010) Use of surface-modified microspheres for setting compensation in multicolor flow cytometry antibodies, cells, flow cytometer/flow cytometry, flow cytometry compensation, fluorescent dyes, multicolor flow cytometry, particles, violet laser-excited reagents
Scientific poster (2011) Acoustic cytometric identification and initial characterization of limbal stem cells isolated from human cornea Attune/Attune NxT, cell culture/expansion, cells, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, PCR/qPCR, stem cell research, tissue, violet laser-excited reagents
Scientific poster (2011) Acoustic cytometry for rare event detection of PNH cells Attune/Attune NxT, cells, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, rare-event detection
Scientific poster (2011) Acoustic flow cytometry for multicolor immunophenotyping analysis antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot, violet laser-excited reagents
Scientific Poster (2011) Application of acoustic cytometry in microbiology Attune/Attune NxT, cell concentration, cell health, flow cytometer/flow cytometry, fluorescent dyes, microbiology, viability
Scientific poster (2011) Cell-based measurements of oxidative stress with a new near infrared emitting probe for reactive oxygen species: Applications for multiplexed evaluation of cell health ArrayScan, cell health, cells, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress, viability
Scientific poster (2011) Characterization of human limbal stem cells using acoustic focusing cytometry Attune/Attune NxT, cell culture/expansion, cells, flow cytometer/flow cytometry, fluorescent dyes, rare-event detection, stem cell research, tissue, violet laser-excited reagents
Scientific poster (2011) Detection of human circulating endothelial cells and mouse plasmacytoid dendritic cells Attune/Attune NxT, cells, endothelial cells, flow cytometer/flow cytometry, fluorescent dyes, rare-event detection
Scientific poster (2011) No-lyse no-wash immunophenotyping using acoustic cytometry Attune/Attune NxT, cells, flow cytometer/flow cytometry, flow cytometry sample preparation, fluorescent dyes, immunophenotyping, nanocrystals, Qdot
Scientific poster (2011) Phospho‐protein detection using acoustic cytometry Alexa Fluor, Attune/Attune NxT, cells, flow cytometer/flow cytometry, flow cytometry sample preparation, fluorescent dyes, protein detection
Scientific poster (2011) Rare event analysis: Detection of mouse plasmacytoid dendritic cells and human circulating endothelial cells Alexa Fluor, Attune/Attune NxT, cells, endothelial cells, flow cytometer/flow cytometry, fluorescent dyes, rare-event detection
Scientific poster (2011) The next step in the analysis of lymphocyte proliferation Alexa Fluor, antibodies, Attune/Attune NxT, cell proliferation, cells, CellTrace, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, live-cell imaging, violet laser-excited reagents
Scientific poster (2012) A novel no-wash, no-lyse method for residual leukocyte enumeration using acoustic focusing flow cytometry and a cell-membrane permeant DNA dye Attune/Attune NxT, cells, flow cytometer/flow cytometry, flow cytometry sample preparation, fluorescent dyes, immunophenotyping, rare-event detection, violet laser-excited reagents
Scientific Poster (2012) Cell-based analysis of oxidative stress, lipid peroxidation and lipid peroxidation-derived protein modifications using fluorescence microscopy flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, high content analysis, lipid peroxidation, microplate reader, oxidative stress, viability
Scientific poster (2012) Characterization of human limbal stem cells Attune/Attune NxT, cells, flow cytometer/flow cytometry, flow cytometry sample preparation, fluorescent dyes, rare-event detection, stem cell research, tissue, violet laser-excited reagents
Scientific poster (2012) High sensitivity applications for the Applied BioSystems Attune Attune/Attune NxT, caspase substrates, cells, flow cytometer/flow cytometry, flow cytometry sample preparation, fluorescent dyes, immunophenotyping, viability
Scientific poster (2012) Studying malaria using the Attune flow cytometer Attune/Attune NxT, cells, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, malaria, parasite identification
Scientific poster (2012) Ultrasmall and beyond: Nuclear holopoloid genome sizing of carnivorous plant species by acoustic focusing cytometry Attune/Attune NxT, cells, flow cytometer/flow cytometry, fluorescent dyes, genome sizing, plants
Scientific poster (2013) Cell-based measurements of oxidative stress with a new near infrared emitting probe for reactive oxygen species: Applications for multiplexed evaluation of cell health apoptosis, ArrayScan, caspase substrates, cell health, cells, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, microplate reader, oxidative stress
Scientific Poster (2013) Cells coming to life: Tools for visualizing immune response Attune/Attune NxT, endocytosis/phagocytosis, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, immunophenotyping, live-cell imaging, pH detection
Scientific poster (2013) Improved click chemistry demonstrating EdU cell proliferation with GFP-expressing cells and R-PE-based immunophenotyping Alexa Fluor, Attune/Attune NxT, cell cycle, cell proliferation, cells, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, fluorescent proteins, immunophenotyping
Scientific poster (2013) Monitor caspase 3/7 activity without cell fixation: A novel apoptosis reagent from Molecular Probes Alexa Fluor, apoptosis, Attune/Attune NxT, caspase substrates, cells, flow cytometer/flow cytometry, fluorescent dyes, multicolor flow cytometry
Scientific poster (2013) Pacific Green dye: A new fluorophore for violet laser excitation Alexa Fluor, antibodies, Attune/Attune NxT, cells, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot, viability, violet laser-excited reagents
Scientific poster (2013) Reactive oxygen probes—A broad range of colors with easier labeling and compatibility with fixation: Novel CellROX reagents from Molecular Probes cells, flow cytometer/flow cytometry, fluorescent dyes, multicolor flow cytometry, oxidative stress
Scientific poster (2013) Reactive oxygen probes—A broad range of colors with easier labeling: Novel CellROX reagents from Molecular Probes cells, flow cytometer/flow cytometry, fluorescent dyes, multicolor flow cytometry, oxidative stress, viability
Scientific Poster (2014) Click-iT EdU Plus for flow cytometry: enhanced compatibility for multiplexing Attune/Attune NxT, cell proliferation, Click-iT, flow cytometer/flow cytometry, multicolor flow cytometry
Scientific poster (2014) Copper-safe Click-iT Plus EdU proliferation assay: Improved compatibility with simultaneous phycobiliprotein and fluorescent protein detection Attune/Attune NxT, cell proliferation, Click-iT, flow cytometer/flow cytometry, multicolor flow cytometry
Scientific poster (2014) Flow cytometry multiplexing used to analyze metabolic activity and assess pharmaceutical compound toxicity as a high-throughput screening tool Attune/Attune NxT, drug discovery, flow cytometer/flow cytometry
Scientific Poster (2015) A no lyse, no-wash approach to characterizing phagocyte phenotype and function in whole human blood on the Attune NxT Flow Cytometer Attune/Attune NxT, cell cycle, flow cytometer/flow cytometry, immunophenotyping, no lyse, phagocytosis, whole blood
Scientific Poster (2016) A microsphere-based universal compensation approach Attune/Attune NxT, Flow cytometer calibration, flow cytometer/flow cytometry, flow cytometry sample preparation, immunophenotyping, multicolor flow cytometry
Scientific Poster (2016) A novel non-cytotoxic fluorescent dye for cell proliferation analysis in flow cytometry Attune/Attune NxT, cell health, cell proliferation, CellTrace, EVOS, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging
Scientific Poster (2016) An objective test to qualify plate samplers for complex flow cytometric assays Attune/Attune NxT, autosampler, flow cytometer/flow cytometry
Scientific Poster (2016) Cell mediated cytotoxicity in “untouched” whole blood Attune/Attune NxT, cell concentration, cell cycle, flow cytometer/flow cytometry, immunophenotyping, no lyse, whole blood
Scientific Poster (2016) No-lyse no-wash new strategy for CD34+ for absolute cell counting without beads antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, flow cytometry compensation, fluorescent dyes, immunophenotyping, multicolor flow cytometry, particles