Articles that detail flow cytometry applications

Flow Cytometry Application Notes

The articles listed below comprise our application notes, scientific posters, and BioProbes Journal articles collections. These resources give overviews of specific flow cytometry applications and as well as experimental design details and data and results. 


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TypeTitleCategory
Application note (2019)Designing a T cell backbone panel using the Attune NxT Flow Cytometer Attune/Attune NxT Flow Cytometer, flow cytometer, flow cytometry, immunophenotyping, multicolor flow cytometry, fluorescent dyes
Application note (2019)The importance of accurate cell counting in flow cytometry and cell sortingflow cytometer/flow cytometry, viability, cell counting, automated cell counter, Countess
Application note (2018)A guide to analyze Attune NxT FCS files in ModFit LT software Attune/Attune NxT Flow Cytometer, flow cytometer, flow cytometry, Attune NxT Software
Application note (2018)A guide to analyzing Attune NxT FCS data files using FlowJo software Attune/Attune NxT Flow Cytometer, flow cytometer, flow cytometry, Attune NxT Software
Application note (2018)High-throughput automation with the Attune NxT Autosampler: consistent results across all wells and across plates Attune/Attune NxT Flow Cytometer, flow cytometer, flow cytometry, multicolor flow cytometry, Autosampler
Application note (2018)Using the Attune NxT Flow Cytometer for rapid and accurate analysis of nuclear DNA content in plants Attune NxT Flow Cytometer, plants
Application note (2018)High-throughput automation with the Attune NxT Autosampler: consistent results across all wells and across plates Attune/Attune NxT Flow Cytometer, Autosampler
Application note (2018)A guide to analyze Attune NxT FCS data files in FCS Express 6 Software Attune/Attune NxT Flow Cytometer, Attune NxT software, flow cytometry
Application note (2017)Recommendations for accurate concentration measurements on the Attune NxT Flow CytometerAttune/Attune NxT Flow Cytometer, flow cytometry, cell concentration, cell counting
Application note (2017)Flow cytometry analysis of transcription factor expression during differentiation of hPSC-derived cardiomyocytesAttune/Attune NxT Flow Cytometer, cell differentiation, flow cytometry, multicolor flow cytometry, stem cell research
Application note (2017)Validated 10-color T cell panel using the Attune NxT Flow Cytometerantibodies, Attune/Attune NxT Flow Cytometer, flow cytometry, immunology, immunophenotyping, fluorescent dyes, multicolor flow cytometry
Application note (2017)Flow cytometry analysis of dose response for apoptosis induction apoptosis, Attune NxT, Autosampler, flow cytometry, cell toxicity, CellEvent Caspase-3/7 Green 
Application note (2017)Recommendations for accurate carryover measurements on the Attune NxT Flow Cytometer Attune/Attune NxT Flow Cytometer, flow cytometer, flow cytometry, cell concentration, cell counting
Application note (2016)Detection of fluorescent proteins using the Attune NxT Flow Cytometer Attune/Attune NxT Flow Cytometer, flow cytometer, flow cytometry, multicolor flow cytometry, fluorescent dyes, fluorescent protein
Application note (2016)Detection of platelets in whole blood using the Attune NxT Flow Cytometer Attune NxT, flow cytometer, platelets, immunophenotyping, no-wash, no-lyse
Application note (2015)Attune NxT Flow Cytometer for 6-color immunophenotyping analysis of stained human whole blood using a no-lyse, no-wash protocol, with no compensationAttune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry
Application note (2015)Detection of murine regulatory T cells on the Attune NxT Flow CytometerAttune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry
Application note (2015)Multiparameter analysis of murine regulatory T cells and dendritic cells with the Attune NxT Flow CytometerAttune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry
Application note (2015)No-wash, no-lyse detection of leukocytes in human whole blood on the Attune NxT Flow Cytometer Attune/Attune NxT Flow Cytometer, flow cytometer, flow cytometry, immunophenotyping, multicolor flow cytometry, fluorescent dyes
Application note (2015)Ten-parameter immunophenotyping of human lysed whole blood with the Attune NxT Flow Cytometer allows identification of multiple T cell subsets and myeloid cellsAttune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry
Application note (2015)13-parameter immunophenotyping of human lysed whole blood with the Attune NxT Flow Cytometer allows for the identification of B cells, NK cells, multiple T cell subsets and myeloid cells Alexa Fluor, antibodies, Attune NxT, compensation, flow cytometer, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot
Application note (2015)Live/dead algal analysis with the Attune NxT Flow Cytometer algae, Attune NxT, flow cytometer, flow cytometry assays, fluorescent dyes, microbiology, viability
Application note (2015)No-wash, no-lyse detection of phagocytic cells via a pHrodo BioParticles functional assay in human whole blood on the Attune NxT Flow Cytometer antibodies, Attune NxT, flow cytometer, flow cytometry sample preparation, fluorescent dyes, immunology, immunophenotyping, particles, pH detection
BioProbes articles (Issues 50– present day)BioProbes Journal of Cell Biology Applicationcell analysis, flow cytometry, imaging microscopy, immunoassays, antibodies, protein detection and quantification
Blog postClog resistance of non-pressure based flow cytometersAttune NxT, flow cytometer
Blog postSuper Bright conjugates for flow cytometryantibodies, Attune NxT, flow cytometry, immunophenotyping, multicolor flow cytometry, Super Bright
Blog postWhat are event rates and should you always trust the “Maximum Event Rate” of your flow cytometer?Attune NxT, flow cytometer, instrument set-up
Blog postFlow cytometer fluidics and why they are not all the sameAttune NxT, flow cytometer, instrument set-up
FluorophorePacific Blue dyeantibody conjugates, flow cytometry, immunophenotyping, violet laser
FluorophorePacific Green dyeantibody conjugates, flow cytometry, immunophenotyping, violet laser
FluorophorePacific Orange dyeantibody conjugates, flow cytometry, immunophenotyping, violet laser
FluorophoreSuper Bright 436 dyeantibody conjugates, flow cytometry, immunophenotyping, tandem polymer dyes, violet laser
FluorophoreSuper Bright 600 dyeantibody conjugates, flow cytometry, immunophenotyping, tandem polymer dyes, violet laser
FluorophoreSuper Bright 645 dyeantibody conjugates, flow cytometry, immunophenotyping, tandem polymer dyes, violet laser
FluorophoreSuper Bright 702 dyeantibody conjugates, flow cytometry, immunophenotyping, tandem polymer dyes, violet laser
FluorophoreAllophycocyanin (APC)antibody conjugates, flow cytometry, immunophenotyping, phycobiliproteins, tandem dyes
FluorophoreR-phycoerythrin (R-PE)antibody conjugates, flow cytometry, immunophenotyping, phycobiliproteins, tandem dyes
FluorophoreCyan fluorescent protein (CFP)flow cytometry, fluorescent proteins
FluorophoreGreen fluorescent protein (GFP)flow cytometry, fluorescent proteins
FluorophoreRed fluorescent protein (RFP)flow cytometry, fluorescent proteins
FluorophoreFluorophore selection guideantibody conjugates, flow cytometry, immunophenotyping
Immunology informationB cell activation functional assayimmunology
Immunology informationCostimulatory and inhibitory interactions control T cell responseimmunology, stimulation, T cells
Immunology informationFoxp3 overviewflow cytometry, Foxp3,intracellular staining
Immunology informationInnate immunity overviewimmunology
Immunology informationInnate lymphoid cells overviewimmunology
Immunology informationMicrobiome (Microbiota) overviewimmunology
Immunology informationNatural killer cells overviewimmunology
Immunology informationT cell activation functional assayimmunology
Immunology informationT follicular helper differentiation functional assayimmunology
Immunology informationT reg differentiation functional assayimmunology
Immunology informationT regulatory cells overviewimmunology
Immunology informationTh1 differentiation functional assayimmunology
Immunology informationTh17 cells overviewimmunology
Immunology informationTh17 differentiation functional assayimmunology
Immunology informationTh2 differentiation functional assayimmunology
Immunology informationTh22 differentiation functional assayimmunology
Immunology informationTh9 differentiation functional assayimmunology
Immunology informationToll-like receptor overview 
Molecular Probes HandbookPhycobiliproteins—Section 6.4antibody labeling, multicolor flow cytometry, tandem conjugates
Molecular Probes HandbookMicrospheres—Section 6.5flow cytometer calibration, fluorescence microscopy/fluorescence imaging, microscope calibration, particles
Molecular Probes HandbookFlow cytometry reference standards—Section 23.2flow cytometer
Molecular Probes HandbookUsing the Fluorescence SpectraViewer—Note 23.1flow cytometry, fluorescence, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins
Molecular Probes HandbookMHC tetramer technology—Note 7.2antibodies, flow cytometry, immunology, Major Histocompatibility Complexes, T cells
Molecular Probes HandbookProbes for mitochondria—Section 12.2antibodies, cell structure-mitochondria, CellLight, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Molecular Probes HandbookProbes for lysosomes, peroxisomes and yeast vacuoles—Section 12.3antibodies, cell structure-lysosomes, CellLight, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Molecular Probes HandbookProbes for the nucleus—Section 12.5antibodies, cell structure-nucleus, CellLight, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, membrane permeability
Molecular Probes HandbookProbes useful at near-neutral pH—Section 20.2BCECF, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, pH detection, pH indicators, SNARF
Molecular Probes HandbookProbes useful at acidic pH—Section 20.3flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo
Molecular Probes HandbookpH indicator conjugates—Section 20.4flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo
Molecular Probes HandbookOverview of pH indicators—Section 20.1fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo
Molecular Probes HandbookNucleic acid stains—Section 8.1cell cycle, cell structure-nucleus, cell structure-plasma membrane, cell viability, DAPI, DNA binding dyes, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, membrane permeability, RNA binding dyes, SYTO, SYTOX
Molecular Probes HandbookTracers for membrane labeling—Section 14.4cell structure-plasma membrane, cell tracking, flow cytometry, fluorescence microscopy/fluorescence imaging, live-cell imaging
Molecular Probes HandbookMicrospheres and Qdot Nanocrystals for tracing—Section 14.6blood flow, FluoSpheres, neuronal tracing, particles, Qdot, Qtracker, quantum dots
Molecular Probes HandbookViability and cytotoxicity assay reagents—Section 15.2cell health, cell viability and function, fluorescence microscopy/fluorescence imaging, microplate reader
Molecular Probes HandbookViability and cytotoxicity assay kits for diverse cell types—Section 15.3cell health, cell viability and function, fluorescence microscopy/fluorescence imaging, microplate reader
Molecular Probes HandbookAssays for apoptosis and autophagy—Section 15.5apoptosis, autophagy, flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging
Molecular Probes HandbookZenon Technology: Versatile reagents for immunolabeling—Section 7.3antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, secondary detection, Zenon
PathwaysAdaptive immunity pathwaysimmunology
PathwaysInnate immunity pathwaysimmunology
PathwaysInflammatory response pathway 
PathwaysInterferon (IFN) cell signaling pathway 
PathwaysT-cell receptor (TCR) cell signaling pathway 
PathwaysToll-like receptor (TLR) cell signaling pathway 
ProtocolsAnnexin V staining protocol for flow cytometry (Invitrogen eBioscience reagents)apoptosis, flow cytometry
ProtocolsBrdU Staining Protocol for Flow Cytometry (Invitrogen eBioscience reagents)cell proliferation, flow cytometry
ProtocolsCell preparation for flow cytometry protocols (Invitrogen eBioscience reagents)flow cytometry, sample preparation
ProtocolsCellTrace CFSE cell proliferation Kit protocolcell proliferation, flow cytometry
ProtocolsCellTrace Far Red cell proliferation Kit protocolcell proliferation, flow cytometry
ProtocolsCellTrace Violet cell proliferation Kit protocolcell proliferation, flow cytometry
ProtocolsLIVE/DEAD Fixable Dead cell stains protocolflow cytometry, viability
ProtocolsLIVE/DEAD Sperm Viability Kit flow cytometry protocolflow cytometry, viability
ProtocolsLIVE/DEAD Violet Viability/Vitality Kit protocolflow cytometry, viability
ProtocolsOneComp and UltraComp Compensation Beads protocols for flow cytometry (Invitrogen eBioscience reagents)compensation, flow cytometry
ProtocolsPharmacological Induction of Apoptosis with Camptothecin (Invitrogen eBioscience reagents)apoptosis, flow cytometry
ProtocolsRed blood cell lysis protocols using eBioscience lysis buffers (Invitrogen eBioscience reagents)flow cytometry, sample preparation
ProtocolsStaining cell surface targets for flow cytometry (Invitrogen eBioscience reagents)extracellular staining, flow cytometry, immunophenotyping
ProtocolsStaining cells with eFluor proliferation dyes for flow cytometry (Invitrogen eBioscience reagents)cell proliferation, flow cytometry
ProtocolsStaining intracellular antigens for flow cytometry (Invitrogen eBioscience reagents)flow cytometry, immunophenotyping, intracellular staining
ProtocolsSYTOX Dead Cell Stains protocolcell viability, flow cytometry
ProtocolsSuper Bright staining buffer protocol antibody conjugates, flow cytometry, immunophenotyping
ProtocolsViability staining protocol for flow cytometry (Invitrogen eBioscience reagents)cell viability, flow cytometry
Scientific poster (2019)Development of in vitro immune effector function assays to better approximate the in vivo behavior of biotherapeutics and cell therapies immune, immunology, flow cytometry, flow cytometer, attune, cell therapy, biotherapeutics
Scientific poster (2019)Functional Flow Cytometry to predict PD-L1 Conformational Change and Improve Cancer Immunotherapy flow cytometry, flow cytometer, attune, dyecycle violet, PD-L1, cancer, immunotherapy, NLNW
Scientific poster (2019)Expression of an immune checkpoint receptor VSIG4 defines new subsets of mouse peritoneal macrophages flow cytometry, flow cytometer, attune, immune checkpoint, VSIG4, macrophates, mouse peritoneal
Scientific poster (2018)Induced pluripotent stem cell sorting, culture and differentiation to desired cell lineage Attune/Attune NxT, cell counting, cell sorting, Countess, EVOS, fluorescence activated cell sorting, iSort Automated Cell Sorter, imaging microscopy, stem cell research
Scientific poster (2018)Modular, defined assembly of antibody-based constructs using click chemistry Alexa Fluor, antibody labeling, Attune/Attune NxT, CellInsight, drug discovery, drug research, high content analysis (HCA), SiteClick, spheroids
Scientific poster (2018)Hypoxia measurements in live and fixed cells using fluorescence microscopy, high-content imaging and flow cytometry Attune/Attune NxT, brightfield microscopy, CellInsight, EVOS, high content analysis (HCA), hypoxia, spheroids
Scientific poster (2018)Evaluation of multiple techniques for PMT optimization Attune/Attune NxT Flow Cytometer, flow cytometer calibration, photomultiplier settings
Scientific poster (2018)PUBLISH OR PERISH? Initiating, maintaining, and growing a publication history within an SRL role flow cytometry, career advancement, shared lab resources
Scientific poster (2018)Prospective cytometric study evaluating the alkaline phosphatase activity as a potential functional biomarker of primitive malignant cells in acute leukemia antibodies, Attune/Attune NxT, cancer, cell function, flow cytometry, immunophenotyping, stem cell research
Scientific poster (2018)Synergistic effect of curcumin in combination with proapoptotic drugs in human cell models for chronic (CLL) lymphocytic leukaemia Attune/Attune NxT Flow Cytometer, cell cycle, cancer, drug development, drug research, flow cytometry
Scientific poster (2018)Differently cell doublet profiles: comparison of light scattering with fluorescence measurement techniques Attune/Attune NxT Flow Cytometer, cell counting, cell cycle, cell viability, flow cytometry, single cell discrimination
Scientific poster (2018)Differential expression of PD-L1 on myeloid derived suppressor cells as a potential screening approach for cancer immunotherapy in multiple myeloma Attune/Attune NxT, cancer, drug research, , immunology, immuno-oncology, multicolor flow cytometry, translational research solutions
Scientific poster (2018)Maintaining even temperature distribution over chilled samples in 96-well plates and micro-centrifuge tubes academic research solutions, microplates, quality control, temperature control
Scientific poster (2018)A comparison of GFP BrightComp eBeads Compensation Bead Kit performance against multiple GFP variants Attune/Attune NxT, beads, flow cytometry compensation, fluorescent protein detection,
Scientific poster (2018)Fluorescent hypoxia sensor for flow cytometry apoptosis, Attune/Attune NxT, brightfield microscopy, EVOS, flow cytometry, hypoxia, immunology
Scientific Poster (2017)T-lymphocyte immunophenotyping: 14-color flow cytometry panel design using the Attune NxT Flow Cytometer and Super Bright fluorescent dyes antibodies, Attune NxT, fluorescent dyes, immunophenotyping, multicolor flow cytometry, particles, Super Bright
Scientific Poster (2016)A novel non-cytotoxic fluorescent dye for cell proliferation analysis in flow cytometry Attune NxT, cell health, cell proliferation, CellTrace, EVOS, flow cytometry, fluorescence microscopy/fluorescence imaging
Scientific Poster (2016)An objective test to qualify plate samplers for complex flow cytometric assays Attune NxT, Autosampler, flow cytometer
Scientific Poster (2016)Cell mediated cytotoxicity in “untouched” whole blood Attune NxT, cell concentration, cell cycle, flow cytometry assays, viability, whole blood
Scientific Poster (2016)No-lyse no-wash new strategy for CD34+ for absolute cell counting without beads antibodies, Attune NxT, cell counting, compensation, flow cytometry assays , fluorescent dyes
Scientific Poster (2015)A no lyse, no-wash approach to characterizing phagocyte phenotype and function in whole human blood on the Attune NxT Flow Cytometer Attune NxT, cell cycle, flow cytometer, flow cytometry assays, immunophenotyping, no lyse, phagocytosis, whole blood
Scientific poster (2014)Copper-safe Click-iT Plus EdU proliferation assay: Improved compatibility with simultaneous phycobiliprotein and fluorescent protein detectioncell proliferation, Click-iT, flow cytometry, immunophenotyping
Scientific poster (2014)Flow cytometry multiplexing used to analyze metabolic activity and assess pharmaceutical compound toxicity as a high-throughput screening toolAttune NxT, drug discovery, flow cytometer, flow cytometry assay
Scientific Poster (2014)Click-iT EdU Plus for flow cytometry: enhanced compatibility for multiplexing Attune NxT, cell proliferation, Click-iT, multicolor flow cytometry
Scientific poster (2013)Cell-based measurements of oxidative stress with a new near infrared emitting probe for reactive oxygen species: Applications for multiplexed evaluation of cell health apoptosis, ArrayScan, caspase substrates, cell health, cells, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, microplate reader, oxidative stress
Scientific Poster (2013)Cells coming to life: Tools for visualizing immune response endocytosis/phagocytosis, flow cytometry, fluorescence microscopy/fluorescence imaging, live-cell imaging, pH detection, T cell stimulation
Scientific poster (2013)Improved click chemistry demonstrating EdU cell proliferation with GFP-expressing cells and R-PE-based immunophenotyping Alexa Fluor, cell cycle, cell proliferation, Click-iT, flow cytometry assays, fluorescent dyes, fluorescent proteins, immunophenotyping
Scientific poster (2013)Monitor caspase 3/7 activity without cell fixation: A novel apoptosis reagent Alexa Fluor, apoptosis, Attune NxT, caspase substrates, cells, fluorescent dyes, multicolor flow cytometry
Scientific poster (2013)Reactive oxygen probes—A broad range of colors with easier labeling and compatibility with fixation: Novel CellROX reagents cells, fluorescent dyes, multicolor flow cytometry, oxidative stress
Scientific poster (2013)Reactive oxygen probes—A broad range of colors with easier labeling: Novel CellROX reagents cells, fluorescent dyes, multicolor flow cytometry, oxidative stress, viability
Scientific poster (2011)Rare event analysis: Detection of mouse plasmacytoid dendritic cells and human circulating endothelial cells Alexa Fluor, endothelial cells, flow cytometry, fluorescent dyes, rare event detection
Scientific poster (2011)The next step in the analysis of lymphocyte proliferation Alexa Fluor, antibodies, Attune NxT, cell proliferation, CellTrace, flow cytometry assays, fluorescent dyes, live-cell imaging, violet laser-excited reagents
Scientific poster (2010)A comparison of three techniques to induce efficient ex vivo T cell expansion Alexa Fluor, antibodies, cell proliferation, CellTrace, flow cytometry, fluorescent dyes, immunology, magnetic beads, multicolor flow cytometry, viability
Scientific poster (2010)Analysis of proliferation dynamics in primary and immortalized human cells Alexa Fluor, cancer, cell proliferation, CellTrace, circulatory system, Click-iT, flow cytometry, fluorescent dyes, fluorescent proteins, nucleic acid quantitation
Scientific poster (2010)Dual pulse labeling using EdU and BrdU to monitor alterations in cellular proliferation by fluorescence microscopy and flow cytometry Alexa Fluor, cell cycle, cell proliferation, cells, Click-iT, drug discovery, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Scientific poster (2010)Functional characterization of a novel fluorescent dye for proliferation analysis Alexa Fluor, cell proliferation, cells, CellTrace, flow cytometry, fluorescent dyes, violet laser-excited reagents
Scientific poster (2010)Non-cytotoxic near-IR DNA stain for cell cycle analysis in living cells cell cycle, flow cytometry, fluorescent dyes, fluorescent proteins, live-cell imaging, nucleic acid labeling, viability
Scientific poster (2010)Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry and imaging Alexa Fluor, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, immunofluorescence (IF), immunophenotyping, stem cell research, violet laser-excited reagents
Scientific poster (2010)Simultaneous analysis of cell death mechanisms and oxidative stress using Molecular Probes next generation reagents for imaging and flow cytometry apoptosis, autophagy, caspase substrates, cells, flow cytometry/flow cytometer, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, oxidative stress
Scientific poster (2010)Use of surface-modified microspheres for setting compensation in multicolor flow cytometry antibodies, cells, flow cytometry compensation, fluorescent dyes, multicolor flow cytometry, particles, violet laser-excited reagents
Scientific poster (2009)A comparative study of 25 different CD4 conjugates Alexa Fluor, antibodies, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot
Scientific poster (2009)A novel xeno-free and serum-free culture medium for the expansion of human mesenchymal stem cells cell culture, cell expansion, flow cytometry, fluorescent dyes, gene expression, immunophenotyping, stem cell research
Scientific poster (2009)A simple, fast and quantitative single-step dead-cell indicator for flow cytometry apoptosis, fluorescent dyes, multicolor flow cytometry, nucleic acid quantitation, viability
Scientific poster (2009)Dead cell stains in flow cytometry: A comprehensive analysis apoptosis, cells, flow cytometry, fluorescent dyes, immunophenotyping, viability
Scientific poster (2009)Dual pulse labeling of S-phase population using click chemistry to measure changes in cell proliferation Alexa Fluor, cancer, cell cycle, cell proliferation, cells, Click-iT, flow cytometry, fluorescent dyes
Scientific poster (2009)Dual pulse labeling using a new thymidine analog 5-ethynyl-2’-deoxyuridine (EdU) to detect alterations in S-phase progression by fluorescence microscopy and flow cytometry Alexa Fluor, cell cycle, cell proliferation, cells, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Scientific poster (2009)Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry Alexa Fluor, cell proliferation, cells, Click-iT, flow cytometry, fluorescent dyes, fluorescent proteins, immunophenotyping, nucleic acid quantitation, stem cell research, violet laser-excited reagents
Scientific poster (2008)A new 405 nm excitable fluorescent probe of intracellular thiols for imaging and flow cytometric assays ArrayScan, cells, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, microplate reader, violet laser-excited reagents
Scientific poster (2008)Characterization of DNA content, cyclin B1 and phosphorylated histone H3 with direct S-phase using EDU incorporation in multiparameter testing of cell lines with cell cycle blocking agents Alexa Fluor, antibodies, apoptosis, ArrayScan, cell cycle, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Scientific poster (2008)Detection of DNA synthesis by automated microscopy and image analysis: Comparison of BRDU method and a new click chemistry-based EDU method Alexa Fluor, antibodies, ArrayScan, cell cycle, cell proliferation, cells, Click-it, flow cytometry, fluorescent dyes, microplate reader, nucleic acid labeling
Scientific poster (2008)Detection of S-phase cell cycle progression using 5-ethynyl-2'-deoxyuridine incorporation with click chemistry Alexa Fluor, antibodies, ArrayScan, cell cycle, cells, Click-iT, flow cytometry, fluorescent dyes, microplate reader, nucleic acid labeling, nucleic acid quantitation
Scientific poster (2008)Effects of the thymidine analogues EdU and BrdU on cell viability and cycle progression Alexa Fluor, cell cycle, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, viability
Scientific poster (2008)Zenon labeling technology facilitates detection of intra-cellular phosphoprotein determinants Alexa Fluor, antibodies, cell signaling, cells, flow cytometry, fluorescent dyes, protein labeling, rare cell detection, Zenon
Scientific poster (2007)A new approach for the detection of intracellular glutathione by fluorescence microscopy and flow cytometry ArrayScan, cells, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, live-cell imaging, microplate reader, viability, violet laser-excited reagents
Scientific poster (2007)Evaluation of click chemistry-based alternative to BrdU antibody labeling in tissue and cultured cells using fluorescence microscopy and flow cytometry antibodies, ArrayScan, cell cycle, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, microplate reader, nucleic acid labeling
Scientific poster (2006)Novel violet-excited reagents for detection of viability and vitality  Alexa Fluor, cell health, CellTrace, flow cytometry, fluorescent dyes, viability, violet laser-excited reagents
Selection guideAntibody fixation considerationssample preparation
Selection guideHuman antibody cross reactivity chartantibody cross-reactivity, flow cytometry
Selection guideMouse antibody cross reactivity chartantibody cross-reactivity, flow cytometry
Selection guideCytokine bioassays guidecytokines, flow cytometry, immunophenotyping, intracellular staining
Selection guideIntracellular staining guide (for mouse & human cytokines and non-cytokine proteins)cytokines, flow cytometry, intracellular staining
Selection guideHuman CD & other cellular antigensflow cytometry, immunology, immunophenotyping, extracellular staining
Selection guideIntracellular staining buffer selection guideflow cytometry, immunophenotyping, intracellular staining
Selection guideMouse CD & other cellular antigensflow cytometry, immunology, immunophenotyping, extracellular staining
Selection guidePhospho flow cytometry antibody clone buffer selection guideflow cytometry, immunophenotyping, intracellular staining, phosphoflow cytometry

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.