Articles that detail flow cytometry applications

Flow Cytometry Application Notes

The articles listed below comprise our application notes, scientific posters, and BioProbes Journal articles collections. These resources give overviews of specific flow cytometry applications and as well as experimental design details and data and results. 


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Type Title Category
Application note (2019) Designing a T cell backbone panel using the Attune NxT Flow Cytometer  Attune/Attune NxT Flow Cytometer, flow cytometer, flow cytometry, immunophenotyping, multicolor flow cytometry, fluorescent dyes
Application note (2019) The importance of accurate cell counting in flow cytometry and cell sorting flow cytometer/flow cytometry, viability, cell counting, automated cell counter, Countess
Application note (2018) A guide to analyze Attune NxT FCS files in ModFit LT software  Attune/Attune NxT Flow Cytometer, flow cytometer, flow cytometry, Attune NxT Software
Application note (2018) A guide to analyzing Attune NxT FCS data files using FlowJo software  Attune/Attune NxT Flow Cytometer, flow cytometer, flow cytometry, Attune NxT Software
Application note (2018) High-throughput automation with the Attune NxT Autosampler: consistent results across all wells and across plates  Attune/Attune NxT Flow Cytometer, flow cytometer, flow cytometry, multicolor flow cytometry, Autosampler
Application note (2018) Using the Attune NxT Flow Cytometer for rapid and accurate analysis of nuclear DNA content in plants  Attune NxT Flow Cytometer, plants
Application note (2018) High-throughput automation with the Attune NxT Autosampler: consistent results across all wells and across plates  Attune/Attune NxT Flow Cytometer, Autosampler
Application note (2018) A guide to analyze Attune NxT FCS data files in FCS Express 6 Software  Attune/Attune NxT Flow Cytometer, Attune NxT software, flow cytometry
Application note (2017) Recommendations for accurate concentration measurements on the Attune NxT Flow Cytometer Attune/Attune NxT Flow Cytometer, flow cytometry, cell concentration, cell counting
Application note (2017) Flow cytometry analysis of transcription factor expression during differentiation of hPSC-derived cardiomyocytes Attune/Attune NxT Flow Cytometer, cell differentiation, flow cytometry, multicolor flow cytometry, stem cell research
Application note (2017) Validated 10-color T cell panel using the Attune NxT Flow Cytometer antibodies, Attune/Attune NxT Flow Cytometer, flow cytometry, immunology, immunophenotyping, fluorescent dyes, multicolor flow cytometry
Application note (2017) Flow cytometry analysis of dose response for apoptosis induction  apoptosis, Attune NxT, Autosampler, flow cytometry, cell toxicity, CellEvent Caspase-3/7 Green 
Application note (2017) Recommendations for accurate carryover measurements on the Attune NxT Flow Cytometer  Attune/Attune NxT Flow Cytometer, flow cytometer, flow cytometry, cell concentration, cell counting
Application note (2016) Detection of fluorescent proteins using the Attune NxT Flow Cytometer  Attune/Attune NxT Flow Cytometer, flow cytometer, flow cytometry, multicolor flow cytometry, fluorescent dyes, fluorescent protein
Application note (2016) Detection of platelets in whole blood using the Attune NxT Flow Cytometer  Attune NxT, flow cytometer, platelets, immunophenotyping, no-wash, no-lyse
Application note (2015) Attune NxT Flow Cytometer for 6-color immunophenotyping analysis of stained human whole blood using a no-lyse, no-wash protocol, with no compensation Attune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry
Application note (2015) Detection of murine regulatory T cells on the Attune NxT Flow Cytometer Attune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry
Application note (2015) Multiparameter analysis of murine regulatory T cells and dendritic cells with the Attune NxT Flow Cytometer Attune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry
Application note (2015) No-wash, no-lyse detection of leukocytes in human whole blood on the Attune NxT Flow Cytometer  Attune/Attune NxT Flow Cytometer, flow cytometer, flow cytometry, immunophenotyping, multicolor flow cytometry, fluorescent dyes
Application note (2015) Ten-parameter immunophenotyping of human lysed whole blood with the Attune NxT Flow Cytometer allows identification of multiple T cell subsets and myeloid cells Attune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry
Application note (2015) 13-parameter immunophenotyping of human lysed whole blood with the Attune NxT Flow Cytometer allows for the identification of B cells, NK cells, multiple T cell subsets and myeloid cells  Alexa Fluor, antibodies, Attune NxT, compensation, flow cytometer, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot
Application note (2015) Live/dead algal analysis with the Attune NxT Flow Cytometer  algae, Attune NxT, flow cytometer, flow cytometry assays, fluorescent dyes, microbiology, viability
Application note (2015) No-wash, no-lyse detection of phagocytic cells via a pHrodo BioParticles functional assay in human whole blood on the Attune NxT Flow Cytometer  antibodies, Attune NxT, flow cytometer, flow cytometry sample preparation, fluorescent dyes, immunology, immunophenotyping, particles, pH detection
BioProbes articles (Issues 50– present day) BioProbes Journal of Cell Biology Application cell analysis, flow cytometry, imaging microscopy, immunoassays, antibodies, protein detection and quantification
Blog post Clog resistance of non-pressure based flow cytometers Attune NxT, flow cytometer
Blog post Super Bright conjugates for flow cytometry antibodies, Attune NxT, flow cytometry, immunophenotyping, multicolor flow cytometry, Super Bright
Blog post What are event rates and should you always trust the “Maximum Event Rate” of your flow cytometer? Attune NxT, flow cytometer, instrument set-up
Blog post Flow cytometer fluidics and why they are not all the same Attune NxT, flow cytometer, instrument set-up
Fluorophore Pacific Blue dye antibody conjugates, flow cytometry, immunophenotyping, violet laser
Fluorophore Pacific Green dye antibody conjugates, flow cytometry, immunophenotyping, violet laser
Fluorophore Pacific Orange dye antibody conjugates, flow cytometry, immunophenotyping, violet laser
Fluorophore Super Bright 436 dye antibody conjugates, flow cytometry, immunophenotyping, tandem polymer dyes, violet laser
Fluorophore Super Bright 600 dye antibody conjugates, flow cytometry, immunophenotyping, tandem polymer dyes, violet laser
Fluorophore Super Bright 645 dye antibody conjugates, flow cytometry, immunophenotyping, tandem polymer dyes, violet laser
Fluorophore Super Bright 702 dye antibody conjugates, flow cytometry, immunophenotyping, tandem polymer dyes, violet laser
Fluorophore Allophycocyanin (APC) antibody conjugates, flow cytometry, immunophenotyping, phycobiliproteins, tandem dyes
Fluorophore R-phycoerythrin (R-PE) antibody conjugates, flow cytometry, immunophenotyping, phycobiliproteins, tandem dyes
Fluorophore Cyan fluorescent protein (CFP) flow cytometry, fluorescent proteins
Fluorophore Green fluorescent protein (GFP) flow cytometry, fluorescent proteins
Fluorophore Red fluorescent protein (RFP) flow cytometry, fluorescent proteins
Fluorophore Fluorophore selection guide antibody conjugates, flow cytometry, immunophenotyping
Immunology information B cell activation functional assay immunology
Immunology information Costimulatory and inhibitory interactions control T cell response immunology, stimulation, T cells
Immunology information Foxp3 overview flow cytometry, Foxp3,intracellular staining
Immunology information Innate immunity overview immunology
Immunology information Innate lymphoid cells overview immunology
Immunology information Microbiome (Microbiota) overview immunology
Immunology information Natural killer cells overview immunology
Immunology information T cell activation functional assay immunology
Immunology information T follicular helper differentiation functional assay immunology
Immunology information T reg differentiation functional assay immunology
Immunology information T regulatory cells overview immunology
Immunology information Th1 differentiation functional assay immunology
Immunology information Th17 cells overview immunology
Immunology information Th17 differentiation functional assay immunology
Immunology information Th2 differentiation functional assay immunology
Immunology information Th22 differentiation functional assay immunology
Immunology information Th9 differentiation functional assay immunology
Immunology information Toll-like receptor overview  
Molecular Probes Handbook Phycobiliproteins—Section 6.4 antibody labeling, multicolor flow cytometry, tandem conjugates
Molecular Probes Handbook Microspheres—Section 6.5 flow cytometer calibration, fluorescence microscopy/fluorescence imaging, microscope calibration, particles
Molecular Probes Handbook Flow cytometry reference standards—Section 23.2 flow cytometer
Molecular Probes Handbook Using the Fluorescence SpectraViewer—Note 23.1 flow cytometry, fluorescence, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins
Molecular Probes Handbook MHC tetramer technology—Note 7.2 antibodies, flow cytometry, immunology, Major Histocompatibility Complexes, T cells
Molecular Probes Handbook Probes for mitochondria—Section 12.2 antibodies, cell structure-mitochondria, CellLight, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Molecular Probes Handbook Probes for lysosomes, peroxisomes and yeast vacuoles—Section 12.3 antibodies, cell structure-lysosomes, CellLight, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Molecular Probes Handbook Probes for the nucleus—Section 12.5 antibodies, cell structure-nucleus, CellLight, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, membrane permeability
Molecular Probes Handbook Probes useful at near-neutral pH—Section 20.2 BCECF, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, pH detection, pH indicators, SNARF
Molecular Probes Handbook Probes useful at acidic pH—Section 20.3 flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo
Molecular Probes Handbook pH indicator conjugates—Section 20.4 flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo
Molecular Probes Handbook Overview of pH indicators—Section 20.1 fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo
Molecular Probes Handbook Nucleic acid stains—Section 8.1 cell cycle, cell structure-nucleus, cell structure-plasma membrane, cell viability, DAPI, DNA binding dyes, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, membrane permeability, RNA binding dyes, SYTO, SYTOX
Molecular Probes Handbook Tracers for membrane labeling—Section 14.4 cell structure-plasma membrane, cell tracking, flow cytometry, fluorescence microscopy/fluorescence imaging, live-cell imaging
Molecular Probes Handbook Microspheres and Qdot Nanocrystals for tracing—Section 14.6 blood flow, FluoSpheres, neuronal tracing, particles, Qdot, Qtracker, quantum dots
Molecular Probes Handbook Viability and cytotoxicity assay reagents—Section 15.2 cell health, cell viability and function, fluorescence microscopy/fluorescence imaging, microplate reader
Molecular Probes Handbook Viability and cytotoxicity assay kits for diverse cell types—Section 15.3 cell health, cell viability and function, fluorescence microscopy/fluorescence imaging, microplate reader
Molecular Probes Handbook Assays for apoptosis and autophagy—Section 15.5 apoptosis, autophagy, flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging
Molecular Probes Handbook Zenon Technology: Versatile reagents for immunolabeling—Section 7.3 antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, secondary detection, Zenon
Pathways Adaptive immunity pathways immunology
Pathways Innate immunity pathways immunology
Pathways Inflammatory response pathway  
Pathways Interferon (IFN) cell signaling pathway  
Pathways T-cell receptor (TCR) cell signaling pathway  
Pathways Toll-like receptor (TLR) cell signaling pathway  
Protocols Annexin V staining protocol for flow cytometry (Invitrogen eBioscience reagents) apoptosis, flow cytometry
Protocols BrdU Staining Protocol for Flow Cytometry (Invitrogen eBioscience reagents) cell proliferation, flow cytometry
Protocols cell Preparation for flow cytometry protocols (Invitrogen eBioscience reagents) flow cytometry, sample preparation
Protocols CellTrace CFSE cell proliferation Kit protocol cell proliferation, flow cytometry
Protocols CellTrace Far Red cell proliferation Kit protocol cell proliferation, flow cytometry
Protocols CellTrace Violet cell proliferation Kit protocol cell proliferation, flow cytometry
Protocols LIVE/DEAD Fixable Dead cell stains protocol flow cytometry, viability
Protocols LIVE/DEAD Sperm Viability Kit flow cytometry protocol flow cytometry, viability
Protocols LIVE/DEAD Violet Viability/Vitality Kit protocol flow cytometry, viability
Protocols OneComp and UltraComp Compensation Beads protocols for flow cytometry (Invitrogen eBioscience reagents) compensation, flow cytometry
Protocols Pharmacological Induction of Apoptosis with Camptothecin (Invitrogen eBioscience reagents) apoptosis, flow cytometry
Protocols Red blood cell lysis protocols using eBioscience lysis buffers (Invitrogen eBioscience reagents) flow cytometry, sample preparation
Protocols Staining cell surface targets for flow cytometry (Invitrogen eBioscience reagents) extracellular staining, flow cytometry, immunophenotyping
Protocols Staining cells with eFluor proliferation dyes for flow cytometry (Invitrogen eBioscience reagents) cell proliferation, flow cytometry
Protocols Staining intracellular antigens for flow cytometry (Invitrogen eBioscience reagents) flow cytometry, immunophenotyping, intracellular staining
Protocols SYTOX Dead Cell Stains protocol cell viability, flow cytometry
Protocols Super Bright staining buffer protocol  antibody conjugates, flow cytometry, immunophenotyping
Protocols Viability staining protocol for flow cytometry (Invitrogen eBioscience reagents) cell viability, flow cytometry
Scientific poster (2019) Development of in vitro immune effector function assays to better approximate the in vivo behavior of biotherapeutics and cell therapies  immune, immunology, flow cytometry, flow cytometer, attune, cell therapy, biotherapeutics
Scientific poster (2019) Functional Flow Cytometry to predict PD-L1 Conformational Change and Improve Cancer Immunotherapy  flow cytometry, flow cytometer, attune, dyecycle violet, PD-L1, cancer, immunotherapy, NLNW
Scientific poster (2019) Expression of an immune checkpoint receptor VSIG4 defines new subsets of mouse peritoneal macrophages  flow cytometry, flow cytometer, attune, immune checkpoint, VSIG4, macrophates, mouse peritoneal
Scientific poster (2018) Induced pluripotent stem cell sorting, culture and differentiation to desired cell lineage  Attune/Attune NxT, cell counting, cell sorting, Countess, EVOS, fluorescence activated cell sorting, iSort Automated Cell Sorter, imaging microscopy, stem cell research
Scientific poster (2018) Modular, defined assembly of antibody-based constructs using click chemistry  Alexa Fluor, antibody labeling, Attune/Attune NxT, CellInsight, drug discovery, drug research, high content analysis (HCA), SiteClick, spheroids
Scientific poster (2018) Hypoxia measurements in live and fixed cells using fluorescence microscopy, high-content imaging and flow cytometry  Attune/Attune NxT, brightfield microscopy, CellInsight, EVOS, high content analysis (HCA), hypoxia, spheroids
Scientific poster (2018) Evaluation of multiple techniques for PMT optimization  Attune/Attune NxT Flow Cytometer, flow cytometer calibration, photomultiplier settings
Scientific poster (2018) PUBLISH OR PERISH? Initiating, maintaining, and growing a publication history within an SRL role  flow cytometry, career advancement, shared lab resources
Scientific poster (2018) Prospective cytometric study evaluating the alkaline phosphatase activity as a potential functional biomarker of primitive malignant cells in acute leukemia  antibodies, Attune/Attune NxT, cancer, cell function, flow cytometry, immunophenotyping, stem cell research
Scientific poster (2018) Synergistic effect of curcumin in combination with proapoptotic drugs in human cell models for chronic (CLL) lymphocytic leukaemia  Attune/Attune NxT Flow Cytometer, cell cycle, cancer, drug development, drug research, flow cytometry
Scientific poster (2018) Differently cell doublet profiles: comparison of light scattering with fluorescence measurement techniques  Attune/Attune NxT Flow Cytometer, cell counting, cell cycle, cell viability, flow cytometry, single cell discrimination
Scientific poster (2018) Differential expression of PD-L1 on myeloid derived suppressor cells as a potential screening approach for cancer immunotherapy in multiple myeloma  Attune/Attune NxT, cancer, drug research, , immunology, immuno-oncology, multicolor flow cytometry, translational research solutions
Scientific poster (2018) Maintaining even temperature distribution over chilled samples in 96-well plates and micro-centrifuge tubes  academic research solutions, microplates, quality control, temperature control
Scientific poster (2018) A comparison of GFP BrightComp eBeads Compensation Bead Kit performance against multiple GFP variants  Attune/Attune NxT, beads, flow cytometry compensation, fluorescent protein detection,
Scientific poster (2018) Fluorescent hypoxia sensor for flow cytometry  apoptosis, Attune/Attune NxT, brightfield microscopy, EVOS, flow cytometry, hypoxia, immunology
Scientific Poster (2017) T-lymphocyte immunophenotyping: 14-color flow cytometry panel design using the Attune NxT Flow Cytometer and Super Bright fluorescent dyes  antibodies, Attune NxT, fluorescent dyes, immunophenotyping, multicolor flow cytometry, particles, Super Bright
Scientific Poster (2016) A novel non-cytotoxic fluorescent dye for cell proliferation analysis in flow cytometry  Attune NxT, cell health, cell proliferation, CellTrace, EVOS, flow cytometry, fluorescence microscopy/fluorescence imaging
Scientific Poster (2016) An objective test to qualify plate samplers for complex flow cytometric assays  Attune NxT, Autosampler, flow cytometer
Scientific Poster (2016) Cell mediated cytotoxicity in “untouched” whole blood  Attune NxT, cell concentration, cell cycle, flow cytometry assays, viability, whole blood
Scientific Poster (2016) No-lyse no-wash new strategy for CD34+ for absolute cell counting without beads  antibodies, Attune NxT, cell counting, compensation, flow cytometry assays , fluorescent dyes
Scientific Poster (2015) A no lyse, no-wash approach to characterizing phagocyte phenotype and function in whole human blood on the Attune NxT Flow Cytometer  Attune NxT, cell cycle, flow cytometer, flow cytometry assays, immunophenotyping, no lyse, phagocytosis, whole blood
Scientific poster (2014) Copper-safe Click-iT Plus EdU proliferation assay: Improved compatibility with simultaneous phycobiliprotein and fluorescent protein detection cell proliferation, Click-iT, flow cytometry, immunophenotyping
Scientific poster (2014) Flow cytometry multiplexing used to analyze metabolic activity and assess pharmaceutical compound toxicity as a high-throughput screening tool Attune NxT, drug discovery, flow cytometer, flow cytometry assay
Scientific Poster (2014) Click-iT EdU Plus for flow cytometry: enhanced compatibility for multiplexing  Attune NxT, cell proliferation, Click-iT, multicolor flow cytometry
Scientific poster (2013) Cell-based measurements of oxidative stress with a new near infrared emitting probe for reactive oxygen species: Applications for multiplexed evaluation of cell health  apoptosis, ArrayScan, caspase substrates, cell health, cells, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, microplate reader, oxidative stress
Scientific Poster (2013) Cells coming to life: Tools for visualizing immune response  endocytosis/phagocytosis, flow cytometry, fluorescence microscopy/fluorescence imaging, live-cell imaging, pH detection, T cell stimulation
Scientific poster (2013) Improved click chemistry demonstrating EdU cell proliferation with GFP-expressing cells and R-PE-based immunophenotyping  Alexa Fluor, cell cycle, cell proliferation, Click-iT, flow cytometry assays, fluorescent dyes, fluorescent proteins, immunophenotyping
Scientific poster (2013) Monitor caspase 3/7 activity without cell fixation: A novel apoptosis reagent  Alexa Fluor, apoptosis, Attune NxT, caspase substrates, cells, fluorescent dyes, multicolor flow cytometry
Scientific poster (2013) Reactive oxygen probes—A broad range of colors with easier labeling and compatibility with fixation: Novel CellROX reagents  cells, fluorescent dyes, multicolor flow cytometry, oxidative stress
Scientific poster (2013) Reactive oxygen probes—A broad range of colors with easier labeling: Novel CellROX reagents  cells, fluorescent dyes, multicolor flow cytometry, oxidative stress, viability
Scientific poster (2011) Rare event analysis: Detection of mouse plasmacytoid dendritic cells and human circulating endothelial cells  Alexa Fluor, endothelial cells, flow cytometry, fluorescent dyes, rare event detection
Scientific poster (2011) The next step in the analysis of lymphocyte proliferation  Alexa Fluor, antibodies, Attune NxT, cell proliferation, CellTrace, flow cytometry assays, fluorescent dyes, live-cell imaging, violet laser-excited reagents
Scientific poster (2010) A comparison of three techniques to induce efficient ex vivo T cell expansion  Alexa Fluor, antibodies, cell proliferation, CellTrace, flow cytometry, fluorescent dyes, immunology, magnetic beads, multicolor flow cytometry, viability
Scientific poster (2010) Analysis of proliferation dynamics in primary and immortalized human cells  Alexa Fluor, cancer, cell proliferation, CellTrace, circulatory system, Click-iT, flow cytometry, fluorescent dyes, fluorescent proteins, nucleic acid quantitation
Scientific poster (2010) Dual pulse labeling using EdU and BrdU to monitor alterations in cellular proliferation by fluorescence microscopy and flow cytometry  Alexa Fluor, cell cycle, cell proliferation, cells, Click-iT, drug discovery, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Scientific poster (2010) Functional characterization of a novel fluorescent dye for proliferation analysis  Alexa Fluor, cell proliferation, cells, CellTrace, flow cytometry, fluorescent dyes, violet laser-excited reagents
Scientific poster (2010) Non-cytotoxic near-IR DNA stain for cell cycle analysis in living cells  cell cycle, flow cytometry, fluorescent dyes, fluorescent proteins, live-cell imaging, nucleic acid labeling, viability
Scientific poster (2010) Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry and imaging  Alexa Fluor, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, immunofluorescence (IF), immunophenotyping, stem cell research, violet laser-excited reagents
Scientific poster (2010) Simultaneous analysis of cell death mechanisms and oxidative stress using Molecular Probes next generation reagents for imaging and flow cytometry  apoptosis, autophagy, caspase substrates, cells, flow cytometry/flow cytometer, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, oxidative stress
Scientific poster (2010) Use of surface-modified microspheres for setting compensation in multicolor flow cytometry  antibodies, cells, flow cytometry compensation, fluorescent dyes, multicolor flow cytometry, particles, violet laser-excited reagents
Scientific poster (2009) A comparative study of 25 different CD4 conjugates  Alexa Fluor, antibodies, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot
Scientific poster (2009) A novel xeno-free and serum-free culture medium for the expansion of human mesenchymal stem cells  cell culture, cell expansion, flow cytometry, fluorescent dyes, gene expression, immunophenotyping, stem cell research
Scientific poster (2009) A simple, fast and quantitative single-step dead-cell indicator for flow cytometry  apoptosis, fluorescent dyes, multicolor flow cytometry, nucleic acid quantitation, viability
Scientific poster (2009) Dead cell stains in flow cytometry: A comprehensive analysis  apoptosis, cells, flow cytometry, fluorescent dyes, immunophenotyping, viability
Scientific poster (2009) Dual pulse labeling of S-phase population using click chemistry to measure changes in cell proliferation  Alexa Fluor, cancer, cell cycle, cell proliferation, cells, Click-iT, flow cytometry, fluorescent dyes
Scientific poster (2009) Dual pulse labeling using a new thymidine analog 5-ethynyl-2’-deoxyuridine (EdU) to detect alterations in S-phase progression by fluorescence microscopy and flow cytometry  Alexa Fluor, cell cycle, cell proliferation, cells, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Scientific poster (2009) Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry  Alexa Fluor, cell proliferation, cells, Click-iT, flow cytometry, fluorescent dyes, fluorescent proteins, immunophenotyping, nucleic acid quantitation, stem cell research, violet laser-excited reagents
Scientific poster (2008) A new 405 nm excitable fluorescent probe of intracellular thiols for imaging and flow cytometric assays  ArrayScan, cells, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, microplate reader, violet laser-excited reagents
Scientific poster (2008) Characterization of DNA content, cyclin B1 and phosphorylated histone H3 with direct S-phase using EDU incorporation in multiparameter testing of cell lines with cell cycle blocking agents  Alexa Fluor, antibodies, apoptosis, ArrayScan, cell cycle, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Scientific poster (2008) Detection of DNA synthesis by automated microscopy and image analysis: Comparison of BRDU method and a new click chemistry-based EDU method  Alexa Fluor, antibodies, ArrayScan, cell cycle, cell proliferation, cells, Click-it, flow cytometry, fluorescent dyes, microplate reader, nucleic acid labeling
Scientific poster (2008) Detection of S-phase cell cycle progression using 5-ethynyl-2'-deoxyuridine incorporation with click chemistry  Alexa Fluor, antibodies, ArrayScan, cell cycle, cells, Click-iT, flow cytometry, fluorescent dyes, microplate reader, nucleic acid labeling, nucleic acid quantitation
Scientific poster (2008) Effects of the thymidine analogues EdU and BrdU on cell viability and cycle progression  Alexa Fluor, cell cycle, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, viability
Scientific poster (2008) Zenon labeling technology facilitates detection of intra-cellular phosphoprotein determinants  Alexa Fluor, antibodies, cell signaling, cells, flow cytometry, fluorescent dyes, protein labeling, rare cell detection, Zenon
Scientific poster (2007) A new approach for the detection of intracellular glutathione by fluorescence microscopy and flow cytometry  ArrayScan, cells, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, live-cell imaging, microplate reader, viability, violet laser-excited reagents
Scientific poster (2007) Evaluation of click chemistry-based alternative to BrdU antibody labeling in tissue and cultured cells using fluorescence microscopy and flow cytometry  antibodies, ArrayScan, cell cycle, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, microplate reader, nucleic acid labeling
Scientific poster (2006) Novel violet-excited reagents for detection of viability and vitality   Alexa Fluor, cell health, CellTrace, flow cytometry, fluorescent dyes, viability, violet laser-excited reagents
Selection guide Antibody fixation considerations sample preparation
Selection guide Human antibody cross reactivity chart antibody cross-reactivity, flow cytometry
Selection guide Mouse antibody cross reactivity chart antibody cross-reactivity, flow cytometry
Selection guide Cytokine bioassays guide cytokines, flow cytometry, immunophenotyping, intracellular staining
Selection guide Intracellular staining guide (for mouse & human cytokines and non-cytokine proteins) cytokines, flow cytometry, intracellular staining
Selection guide Human CD & other cellular antigens flow cytometry, immunology, immunophenotyping, extracellular staining
Selection guide Intracellular staining buffer selection guide flow cytometry, immunophenotyping, intracellular staining
Selection guide Mouse CD & other cellular antigens flow cytometry, immunology, immunophenotyping, extracellular staining
Selection guide Phospho flow cytometry antibody clone buffer selection guide flow cytometry, immunophenotyping, intracellular staining, phosphoflow cytometry

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.