r-pe

R-phycoerythrin (R-PE) is an intensely bright phycobiliprotein isolated from red algae that exhibits extremely bright red-orange fluorescence with high quantum yields. It is excited by laser lines from 488 to 561 nm, with absorbance maxima at 496, 546, and 565 nm and a fluorescence emission peak at 578 nm. R-PE is a large molecule used for fluorescence-based detection, primarily in flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity but not photostability.

In practical applications, the sensitivity of R-PE conjugates is usually 5 to 10 times greater than those of the corresponding fluorescein conjugates. In addition, R-PE exhibits:

  • Long-wavelength fluorescence emission
  • Large Stokes shifts
  • Quench resistance due to the protein backbone
  • Very high water solubility
  • Homogeneous structure with defined molecular weights

We offer R-PE conjugated to a variety of primary antibodies, secondary antibodies, and streptavidin. In addition, tandem conjugates of phycobiliproteins are available to generate a range of emission signals from a single excitation wavelength.

Phycoerythrin (R-PE) dashboard


Initial brightness

 
 
 
 
 
Phycoerythrin, also known as R-PE, is typically used for flow cytometry, microarrays, ELISA, and other applications that require high sensitivity but not photostability. 
  

Photostability in buffer

 
 
 
 
 

R-phycoerythrin (R-PE) products

We offer a broad range of RPE products, including primary antibodies, secondary antibodies, streptavidin conjugates, and more.

r-pe-data

Flow cytometry histogram showing human mononuclear cells stained with anti-CD4 complexed with Invitrogen R‑phycoerythrin Zenon labeling reagent.

 

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.