r-pe

R-phycoerythrin (R-PE) is an intensely bright phycobiliprotein isolated from red algae that exhibits extremely bright red-orange fluorescence with high quantum yields. It is excited by laser lines from 488 to 561 nm, with absorbance maxima at 496, 546, and 565 nm and a fluorescence emission peak at 578 nm. R-PE is a large molecule used for fluorescence-based detection, primarily in flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity but not photostability.

In practical applications, the sensitivity of R-PE conjugates is usually 5 to 10 times greater than those of the corresponding fluorescein conjugates. In addition, R-PE exhibits:

  • Long-wavelength fluorescence emission
  • Large Stokes shifts
  • Quench resistance due to the protein backbone
  • Very high water solubility
  • Homogeneous structure with defined molecular weights

We offer R-PE conjugated to a variety of primary antibodies, secondary antibodies, and streptavidin. In addition, tandem conjugates of phycobiliproteins are available to generate a range of emission signals from a single excitation wavelength.

Phycoerythrin (R-PE) dashboard


Initial brightness

 
 
 
 
 
Phycoerythrin, also known as R-PE, is typically used for flow cytometry, microarrays, ELISA, and other applications that require high sensitivity but not photostability.  
   

Photostability in buffer

 
 
 
 
 

  158 488–561 TRITC 565 578  
 
Stain index

Laser line

Common filter set

Excitation max

Emission max
 

R-phycoerythrin (R-PE) products

We offer a broad range of RPE products, including primary antibodies, secondary antibodies, streptavidin conjugates, and more.

r-pe-data

Flow cytometry histogram showing human mononuclear cells stained with anti-CD4 complexed with Invitrogen R‑phycoerythrin Zenon labeling reagent.

 

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.