|5||488-561||575/26||565||576||(in buffer) 2||flow cytometry|
R-phycoerythrin (R-PE) is an intensely bright phycobiliprotein isolated from red algae that exhibits extremely bright red-orange fluorescence with high quantum yields. In practical applications, the sensitivity of R-PE conjugates is usually 5 to 10 times greater than those of the corresponding fluorescein conjugates. In addition, R-PE exhibits:
R-PE is a large molecule used for fluorescence-based detection, primarily in flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity, but not photostability. We offer a broad range of R-PE products, including primary antibodies, secondary antibodies, streptavidin, and tandem conjugates.
Figure 2. Flow cytometry dot plots showing CD4 APC positive cells. Normal human peripheral blood cells were unstimulated (left) or stimulated (right) with Cell Stimulation Cocktail (plus protein transport inhibitors). Cells were intracellularly stained with Anti-Human CD4 APC and Rat IgG2a K Isotype Control PE or Anti-Human IL-2 PE. Viable cells, as determined by Fixable Viability Dye eFluor® 780, in the lymphocyte gate were used for analysis.
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
CyTM is a trademark or registered trademark of GE Healthcare.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and is used under license.
For Research Use Only. Not for use in diagnostic procedures.