Alexa Fluor 750 dye is a bright, near-IR–fluorescent dye with excitation ideally suited for the 633 nm laser line or dye-pumped laser excitation. Alexa Fluor 750 dye is pH-insensitive over a wide molar range, helping ensure that you get stable signals in imaging and flow cytometry applications.
Tandem conjugates of Alexa Fluor 750 on primary and secondary antibodies are widely used in flow cytometry.
Alexa Fluor 750 dye is particularly useful in stochastic optical reconstruction microscopy (STORM), where it is an exceptional reporter for both dSTORM and nSTORM.
We offer Alexa Fluor 750 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to enable you to generate your own antibody conjugates or probes.
|Long-wavelength emission ideal for imaging in tissues with background autofluorescence; exceptional reporter for both dSTORM and nSTORM.|
Photostability in buffer
Photostability in antifade
Performance in SRM
We offer a broad range of Alexa Fluor 750 products, including protein and antibody labeling kits, secondary antibodies, bioconjugates and more:
Detail of detyrosinated tubulin stained with Alexa Fluor 750 dye shows the hollow structure generated by primary antibody labeling of microtubules. Image courtesy of Joshua Vaughan et al., University of Washington, Seattle, WA.
Select dye-labeling chemistries for conjugation with the following reactive groups:
|Amine||Aldehyde, ketone, carboxylate or phosphate|
Select an optimized labeling kit for your target antibody or protein:
|Target protein||Kit||Cat. No.|
|Site-specific antibody labeling|
|<1–20 µg rabbit IgG||Zenon Alexa Fluor 750 Rabbit IgG Labeling Kit||Z25312|
|Conventional antibody/protein labeling|
|0.5–3 mg antibody||Alexa Fluor SAIVI 750 Antibody Labeling Kit||S30046|
Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Learn more about the:
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and is used under license.
For Research Use Only. Not for use in diagnostic procedures.