Alexa Fluor 750 dye

Alexa Fluor 750 dye is a bright, near-IR–fluorescent dye with excitation ideally suited for the 633 nm laser line or dye-pumped laser excitation. Alexa Fluor 750 dye is pH-insensitive over a wide molar range, helping ensure that you get stable signals in imaging and flow cytometry applications.

Tandem conjugates of Alexa Fluor 750 on primary and secondary antibodies are widely used in flow cytometry.

Alexa Fluor 750 dye is particularly useful in stochastic optical reconstruction microscopy (STORM), where it is an exceptional reporter for both dSTORM and nSTORM.

We offer Alexa Fluor 750 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to enable you to generate your own antibody conjugates or probes.

Alexa Fluor 750 dye dashboard

Initial brightness		Long-wavelength emission ideal for imaging in tissues with background autofluorescence; exceptional reporter for both dSTORM and nSTORM.

Photostability in buffer

Photostability in antifade

Performance in SRM

	633	Cy7	749	775
	Laser line	Common filter set	Excitation max	Emission max

Alexa Fluor 750 dye products

We offer a broad range of Alexa Fluor 750 products, including protein and antibody labeling kits, secondary antibodies, bioconjugates and more:

See all Alexa Fluor 750 Secondary Antibodies

Detail of detyrosinated tubulin stained with Alexa Fluor 750 dye shows the hollow structure generated by primary antibody labeling of microtubules. Image courtesy of Joshua Vaughan et al., University of Washington, Seattle, WA.

Alexa Fluor 750 reactive dyes

Select dye-labeling chemistries for conjugation with the following reactive groups:

Amine	Aldehyde, ketone, carboxylate or phosphate
NHS ester, 5 mg NHS ester, 25 mg	Maleimide

Alexa Fluor 750 protein/antibody labeling kits

Select an optimized labeling kit for your target antibody or protein:

Target protein	Kit	Cat. No.
Site-specific antibody labeling
<1–20 µg rabbit IgG	Zenon Alexa Fluor 750 Rabbit IgG Labeling Kit	Z25312
Conventional antibody/protein labeling
0.5–3 mg antibody	Alexa Fluor SAIVI 750 Antibody Labeling Kit	S30046