Alexa Fluor 750 dye is a bright, near-IR–fluorescent dye with excitation ideally suited for the 633 nm laser line or dye-pumped laser excitation. Alexa Fluor 750 dye is pH-insensitive over a wide molar range, helping ensure that you get stable signals in imaging and flow cytometry applications.
Tandem conjugates of Alexa Fluor 750 on primary and secondary antibodies are widely used in flow cytometry.
Alexa Fluor 750 dye is particularly useful in stochastic optical reconstruction microscopy (STORM), where it is an exceptional reporter for both dSTORM and nSTORM.
We offer Alexa Fluor 750 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to enable you to generate your own antibody conjugates or probes.
Alexa Fluor 750 dye dashboard
 Initial brightness |
Long-wavelength emission ideal for imaging in tissues with background autofluorescence; exceptional reporter for both dSTORM and nSTORM. | ||
 Photostability in buffer |
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 Photostability in antifade |
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 Performance in SRM |
| 633 | Cy7 | 749 | 775 | ||
 Laser line |
 Common filter set |
 Excitation max |
Alexa Fluor 750 dye products
We offer a broad range of Alexa Fluor 750 products, including labeling kits, secondary antibodies, bioconjugates and more:
- Super-resolution microscopy (SRM) applications
- Antibody labeling kits for direct detection
- Primary antibodies for direct detection
- Secondary antibodies for flexible labeling
- Tandem conjugates for flow cytometry
- Streptavidin conjugates for signal amplification
- Streptavidin tandem conjugates for signal amplification
Detail of detyrosinated tubulin stained with Alexa Fluor 750 dye shows the hollow structure generated by primary antibody labeling of microtubules. Image courtesy of Joshua Vaughan et al., University of Washington, Seattle, WA.
Alexa Fluor 750 reactive dyes
Select dye-labeling chemistries for conjugation with the following reactive groups:
| Amine | Aldehyde, ketone, carboxylate or phosphate |
|---|---|
Alexa Fluor 750 protein/antibody labeling kits
Select an optimized labeling kit for your target antibody or protein:
| Target protein | Kit | Cat. No. |
|---|---|---|
| Site-specific antibody labeling | ||
| <1–20 µg rabbit IgG | Zenon Alexa Fluor 750 Rabbit IgG Labeling Kit | Z25312 |
| Conventional antibody/protein labeling | ||
| 0.5–3 mg antibody | Alexa Fluor SAIVI 750 Antibody Labeling Kit | S30046 |
Alexa Fluor dye family
Illuminating research for over 15 years
Alexa Fluor dyes are among the most trusted fluorescent dyes available today. In addition to superior performance, you’ll get a technical support team experienced in problem-solving, >50,000 published references, application and experimental tips, and protocols to help with experimental planning. For an outstanding research experience, choose Alexa Fluor dyes.
- Learn more about the brightness of Alexa Fluor dyes
- Learn more about the photostability of Alexa Fluor dyes
- Read all about Alexa Fluor dyes in the Molecular Probes Handbook
Resources
Molecular Probes Handbook
The significantly revised 11th Edition is the most complete fluorescence labeling and detection reference available. The Molecular Probes Handbook can help you achieve the best experimental results for your fluorescence labeling and detection experiments. View the handbook online or order your own copy.
Antifade reagents
Antifade reagents suppress photobleaching and preserve the signals of your fluorescently labeled target molecules. They offer excellent compatibility with a multitude of dyes across the spectrum, making them especially valuable tools for multicolor applications. For added convenience, some formulations include the nuclear counterstain DAPI, eliminating the need for a separate counterstaining step.
Learn more about SlowFade Antifade reagents
Learn more about ProLong Antifade reagents
Cell stain tool
Stain your own cell by using our simulation tool to develop reproducible results with many of our signature fluorescent dyes. Develop your multiplexing strategy for antibody probes, reactive dyes, and counterstains to create your perfectly labeled fluorescent cell.
Fluorescence SpectraViewer
Plot and compare spectra and check the spectral compatibility for common fluorophores including all of the fluorescent probes from Thermo Fisher Scientific. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
For Research Use Only. Not for use in diagnostic procedures.
