Invitrogen

CD8 Monoclonal Antibody (3B5), APC-Alexa Fluor 750

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CD8 Antibody in Flow Cytometry (Flow) — Human peripheral blood lymphocytes were stained using APC-Alexa Fluor® 750 of anti-human CD8 monoclonal antibody (clone 3B5). The negative control profiles represent unstained cells.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

Assay-Dependent

Product Specifications

Species Reactivity

Human

Host / Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

3B5

Immunogen

Human CD8

Conjugate

APC-Alexa Fluor® 750

Excitation/Emission Profile

View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with 10% sucrose, BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

RRID

AB_10372357

Product Specific Information

The allophycocyanin (APC)-Alexa Fluor® 750 dye tandem conjugate allows for multicolor measurements using excitation by lasers that emit from 633 to 650 nm.

Target Information

CD8 (Cluster of Differentiation 8) is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediate efficient cell-cell interactions within the immune system. The CD8 antigen acts as a co-receptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 is found on a T cell subset of normal cytotoxic/suppressor cells which make up approximately 20-35 % of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer (NK) cells, subpopulations of peripheral blood null cells, thymocytes and bone marrow cells. The CD8 co-receptor functions as either a homodimer composed of two alpha chains, or as a disulfide-linked heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. The majority of CD8+ T cells express CD8 as a alpha/beta heterodimer. In HIV, the HIV-2 envelope glycoprotein binds CD8 alpha chain (but not the beta chain).

Analyte Specific Reagent

Bioinformatics

Protein Aliases: CD8; CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; Leu-2; Leu2 T-lymphocyte antigen; MAL; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

Gene Aliases: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37

UniProt ID: (Human) P01732, (Human) P10966

Entrez Gene ID: (Human) 925, (Human) 926

Molecular Function: cytokine receptor defense/immunity protein immunoglobulin receptor superfamily receptor