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Invitrogen
CD8 Monoclonal Antibody (3B5), Pacific Blue™
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FIGURE: 1 / 11
CD8 Antibody (MHCD0828) in Flow
Figure 1. Multicolor analysis of CD4-positive and CD8-positive populations using Qdot® 605 streptavidin and a Pacific Blue™ dye-labeled conjugate. Human mononuclear cells were blocked with nonspecific goat IgG and then stained with a biotinylated mouse anti-human CD4 antibody. Cells were incubated with Qdot® 605 streptavidin, then with mouse anti-human CD8 antibody conjugated to Pacific Blue™ dye. Cells were analyzed on a flow cytometer equipped with a 405 nm violet diode laser and 450/50 and 605/20 nm bandpass filters. Compensation was pe... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MHCD0828
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Rhesus monkey
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
3B5
Immunogen
Recognizes the CD8 antigen
Conjugate
Pacific Blue™
Pacific Blue™
Pacific Blue™
Excitation/Emission Max
404/455 nm
View spectra
Form
Liquid
Concentration
0.25 mg/mL
Amount
125 µg
Purification
purified
Storage buffer
PBS with 4mg/mL BSA
Contains
0.1% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
For fluorochrome conjugated antibodies only, light exposure should be avoided. Use dim light during handling, incubation with cells, and prior to analysis. It is recommended that cells be analyzed within 18 hours of staining. If the reagent is being diluted, it is recommended that only the quantity to be used within one week be diluted.
Each lot is tested by flow cytometry using human peripheral blood leukocytes (PBL). Based on this testing it is recommended that 5 µL of antibody be used per 1 x 10^6 cells in a 100 µL staining volume.
Target Information
CD8, also known as cluster of differentiation 8, is a type I transmembrane glycoprotein of the immunoglobulin family that plays a crucial role in T cell differentiation, activation, and signal transduction. It is expressed as either a heterodimer (CD8 alpha beta) or a homodimer (CD8 alpha alpha). The CD8 alpha beta form is predominantly found on the majority of thymocytes and a subpopulation of mature alpha beta TCR T cells, while the CD8 alpha alpha form is expressed on gamma delta TCR T cells, a subset of intestinal intraepithelial lymphocytes (IELs), and dendritic cells. CD8 functions as a co-receptor for major histocompatibility complex class I (MHC-I) molecules, working alongside the T cell receptor (TCR). The CD8 alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes, and dendritic cells as disulfide-linked homodimers of CD8 alpha. Upon ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), CD8 recruits lymphocyte-specific protein tyrosine kinase (Lck), leading to lymphokine production, increased motility, and activation of cytotoxic T lymphocytes (CTLs). Activated CTLs are vital for clearing pathogens and tumor cells. The differentiation of naive CD8+ T cells into CTLs is strongly enhanced by cytokines such as IL-2, IL-12, and TGF-beta1. Through its interactions with MHC-I and association with protein tyrosine kinase p56lck, CD8 plays a significant role in T cell development and the activation of mature T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2
Gene Aliases: CD8A; CD8B; CD8B1; MAL
Entrez Gene ID: (Human) 925, (Human) 926
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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