Alexa Fluor 488 dye

Invitrogen Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 488 dye is pH-insensitive over a wide molar range.

Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 488 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

We offer Alexa Fluor 488 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.

Alexa Fluor 488 dye dashboard

Initial brightness		The brightest and most photostable green dye for sensitive detection in live or fixed cells; commonly used with Alexa Fluor 350, 594, and 647 dyes for multiplexing. It is used in stochastic optical reconstruction microscopy (STORM)—as an activator in nSTORM and a reporter in dSTORM. It is also used extensively to label conjugates for SIM, STED, and two-photon excitation (TPE) microscopy.

Photostability in buffer

Photostability in antifade

Performance in SRM

	488	FITC	490	525
	Laser line	Common filter set	Excitation max	Emission max

Alexa Fluor 488 products

We offer a broad range of Alexa Fluor 488 products including labeling kits, secondary antibodies, bioconjugates, and more:

See all Alexa Fluor 488 Primary Antibodies
See all Alexa Fluor 488 Secondary Antibodies

The actin cytoskeleton of this muntjack fibroblast was visualized using the green-fluorescent Alexa Fluor 488 Phalloidin, and the nucleus was stained with TO-PRO-3 reagent.

Alexa Fluor 488 reactive dyes

Select dye-labeling chemistries for conjugation with the following reactive groups:

Amine	Thiol	Aldehyde, ketone, carboxylate, or phosphate	Click reaction
NHS ester TFP SDP	Maleimide	Cadaverine Hydroxylamine Hydrazine	Azide Alkyne

Alexa Fluor 488 protein/antibody labeling kits

Select an optimized labeling kit for your target antibody or protein:

Target protein	Kit	Cat. No.
Site-specific antibody labeling
<1–20 µg mouse IgG1	Zenon Alexa Fluor 488 Mouse IgG1 Labeling Kit	Z25002
<1–20 µg mouse IgG2a	Zenon Alexa Fluor 488 Mouse IgG2a Labeling Kit	Z25102
<1–20 µg mouse IgG2b	Zenon Alexa Fluor 488 Mouse IgG2b Labeling Kit	Z25202
<1–20 µg human IgG	Zenon Alexa Fluor 488 Human IgG Labeling Kit	Z25402
<1–20 µg rabbit IgG	Zenon Alexa Fluor 488 Rabbit IgG Labeling Kit	Z25302
Conventional antibody/protein labeling
10–20 µg antibody	APEX Alexa Fluor 488 Antibody Labeling Kit	A10468
20–100 µg antibody/protein	Alexa Fluor 488 Microscale Antibody/Protein Labeling Kit	A30006
100 µg antibody/protein	Alexa Fluor 488 Antibody/Protein Labeling Kit	A20181
1 mg antibody/protein	Alexa Fluor 488 Antibody/Protein Labeling Kit	A10235