sho-alexa-488-s000983

Invitrogen Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 488 dye is pH-insensitive over a wide molar range.

Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 488 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

We offer Alexa Fluor 488 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.

Alexa Fluor 488 dye dashboard


Initial brightness
 
 
 
 
 
The brightest and most photostable green dye for sensitive detection in live or fixed cells; commonly used with Alexa Fluor 350, 594, and 647 dyes for multiplexing. It is used in stochastic optical reconstruction microscopy (STORM)—as an activator in nSTORM and a reporter in dSTORM. It is also used extensively to label conjugates for SIM, STED, and two-photon excitation (TPE) microscopy. 
  

Photostability in buffer
 
 
 
 
 
  

Photostability in antifade
 
 
 
 
 
  

Performance in SRM
 
 
 
 
 
 488FITC490525 
 
Laser line
Common filter set
Excitation max


Emission max

 
data.par.25688.image.275.160.1.
The actin cytoskeleton of this muntjack fibroblast was visualized using the green-fluorescent Alexa Fluor 488 Phalloidin, and the nucleus was stained with TO-PRO-3 reagent.

Alexa Fluor 488 reactive dyes

Select dye-labeling chemistries for conjugation with the following reactive groups: 

AmineThiolAldehyde, ketone, carboxylate, or phosphateClick reaction

Alexa Fluor 488 protein/antibody labeling kits

Select an optimized labeling kit for your target antibody or protein:

Target proteinKitCat. No.
Site-specific antibody labeling
<1–20 µg mouse IgG1Zenon Alexa Fluor 488 Mouse IgG1 Labeling KitZ25002
<1–20 µg mouse IgG2aZenon Alexa Fluor 488 Mouse IgG2a Labeling KitZ25102
<1–20 µg mouse IgG2bZenon Alexa Fluor 488 Mouse IgG2b Labeling KitZ25202
<1–20 µg human IgGZenon Alexa Fluor 488 Human IgG Labeling KitZ25402
<1–20 µg rabbit IgGZenon Alexa Fluor 488 Rabbit IgG Labeling KitZ25302
Conventional antibody/protein labeling
10–20 µg antibodyAPEX Alexa Fluor 488 Antibody Labeling KitA10468
20–100 µg antibody/proteinAlexa Fluor 488 Microscale Antibody/Protein Labeling KitA30006
100 µg antibody/proteinAlexa Fluor 488 Antibody/Protein Labeling KitA20181
1 mg antibody/proteinAlexa Fluor 488 Antibody/Protein Labeling KitA10235
Alexa Fluor Dye stained cell

Alexa Fluor dye family

Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Learn more about the:

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and is used under license.

For Research Use Only. Not for use in diagnostic procedures.