graph of emission spectra for eFluor 660 dye

The eFluor 660 dye is configured as an alternative to Alexa Fluor 647 or APC. The spectral properties make it an ideal fluorophore that can be used in any flow cytometry panel incorporating the red laser.

Benefits of eFluor 660 include

  • Direct alternative for APC and Alexa Fluor 647 fluorochromes
  • Stable performance when treated with formaldehyde fixation

eFluor 660 dye dashboard


Initial brightness

 
 
 
 
 
eFluor 660 dye is a high-performance fluorophore developed for use in multicolor flow cytometry. It displays stable performance when treated with formaldehyde fixation.  
   

Photostability in buffer

 
 
 
 
 

  633 660/20 633 669  
 
Laser line

Common filter set

Excitation max

Emission max
 
Flow histogram comparing eFluor 660 dye before and after aldehyde fixation


Effects of fixation on eFluor 660. Mouse splenocytes stained with Anti-CD4 eFluor 660 (clone GK1.5) and analyzed immediately (dotted red line). The sample was then fixed in 2% formaldehyde overnight at 4°C (blue line) and analyzed again.

eFluor 660 dye products

We offer eFluor 660 dye conjugated to primary antibodies for use in flow cytometry.

Learn more here:

Resources

Primary antibodies to match your cytometer's lasers

Cell stain tool

To support your multicolor flow cytometry experiments, our antibody portfolios include a variety of dyes with compatibility to all commonly used flow cytometry lasers.

Fluorescence SpectraViewer

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Plot and compare spectra and check the spectral compatibility for common fluorophores including all of the fluorescent probes from Thermo Fisher Scientific. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.

Go to the Fluorescence SpectraViewer

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.